FastQCFastQC Report
Fri 17 Jun 2016
SRR1527938_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527938_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3241135
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCCG206.313162E-446.00000446
CGACTAT206.313162E-446.00000428
GCGCTAA405.6170393E-946.00000434
ACCGGTA501.6370905E-1146.046
TTACTCG253.4185425E-546.025
CGATTAG253.4185425E-546.031
CGTTAAC301.8624596E-646.033
TTACGCC453.110472E-1046.038
ACCGTAC253.4185425E-546.031
ACGGTAG253.4185425E-546.036
ACGAACT453.110472E-1046.028
TAAGCGC351.0203439E-745.99999639
TAGTCGG351.0203439E-745.99999619
GATTGCG2800.043.53571313
CGCTATA12400.043.4032251
AGGGGAT250650.042.7608229
TATCCGG700.042.71428324
TCGCGTG650.042.4615420
CTATACA228700.042.4499323
TCATACA259350.042.3728563
Produced by FastQC (version 0.11.5)