##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527938_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3241135
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.662523467859252	33.0	31.0	34.0	30.0	34.0
2	32.170273067922196	34.0	31.0	34.0	30.0	34.0
3	32.422651632838495	34.0	31.0	34.0	30.0	34.0
4	35.9627192326145	37.0	35.0	37.0	35.0	37.0
5	36.04576359824568	37.0	35.0	37.0	35.0	37.0
6	36.01319815435025	37.0	35.0	37.0	35.0	37.0
7	36.11784174371015	37.0	36.0	37.0	35.0	37.0
8	36.05914841560133	37.0	36.0	37.0	35.0	37.0
9	37.66498371712378	39.0	38.0	39.0	35.0	39.0
10	37.67607088257663	39.0	38.0	39.0	35.0	39.0
11	38.04777925017008	39.0	38.0	39.0	37.0	39.0
12	38.029249321611104	39.0	38.0	39.0	37.0	39.0
13	37.978543627463836	39.0	38.0	39.0	35.0	39.0
14	38.3815530670583	40.0	38.0	40.0	35.0	40.0
15	37.379848725832154	39.0	36.0	40.0	33.0	40.0
16	37.29075370202105	39.0	36.0	40.0	33.0	40.0
17	37.33799610321693	40.0	36.0	40.0	33.0	40.0
18	37.48616240915605	40.0	36.0	40.0	33.0	40.0
19	37.58703756554417	40.0	36.0	40.0	33.0	40.0
20	37.536704271806016	40.0	36.0	40.0	33.0	40.0
21	37.58479174733542	40.0	36.0	40.0	33.0	40.0
22	37.53642844250548	40.0	36.0	40.0	33.0	40.0
23	37.44364489600094	40.0	36.0	40.0	33.0	40.0
24	37.49741093783505	40.0	36.0	40.0	33.0	40.0
25	37.4684957584303	40.0	36.0	40.0	33.0	40.0
26	37.42022717350558	40.0	36.0	40.0	33.0	40.0
27	37.45731510720781	40.0	36.0	40.0	33.0	40.0
28	37.24475623508432	40.0	36.0	40.0	33.0	40.0
29	37.3381840003579	40.0	35.0	40.0	33.0	40.0
30	37.348492117730366	40.0	35.0	40.0	33.0	40.0
31	37.33067521099861	40.0	35.0	40.0	33.0	40.0
32	37.28915025137799	40.0	35.0	40.0	33.0	40.0
33	37.2197893639111	40.0	35.0	40.0	33.0	40.0
34	37.155965734225816	40.0	35.0	40.0	33.0	40.0
35	37.00673251808394	40.0	35.0	40.0	33.0	40.0
36	36.51496435662199	39.0	35.0	40.0	31.0	40.0
37	36.63625952019894	39.0	35.0	40.0	31.0	40.0
38	36.583432038468004	39.0	35.0	40.0	31.0	40.0
39	36.47864158697493	39.0	35.0	40.0	31.0	40.0
40	36.31946956853078	39.0	35.0	40.0	31.0	40.0
41	36.17500782904754	38.0	35.0	40.0	31.0	40.0
42	35.82502826941796	38.0	35.0	40.0	30.0	40.0
43	35.64423851521149	38.0	35.0	40.0	30.0	40.0
44	35.46595652448911	38.0	35.0	40.0	29.0	40.0
45	35.02056933759316	37.0	34.0	40.0	27.0	40.0
46	34.98833340789569	37.0	34.0	40.0	27.0	40.0
47	34.88644811154117	37.0	34.0	40.0	27.0	40.0
48	34.693781653649104	36.0	34.0	40.0	26.0	40.0
49	34.50852093479599	36.0	34.0	40.0	26.0	40.0
50	34.23452278291401	35.0	34.0	40.0	25.0	40.0
51	34.111872847011924	35.0	34.0	39.0	24.0	40.0
52	33.60173272634432	35.0	33.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	5.0
13	9.0
14	13.0
15	27.0
16	61.0
17	164.0
18	391.0
19	956.0
20	1869.0
21	3171.0
22	5145.0
23	8091.0
24	11911.0
25	17022.0
26	23669.0
27	31318.0
28	41192.0
29	52968.0
30	65500.0
31	78159.0
32	94276.0
33	122188.0
34	193322.0
35	336945.0
36	267277.0
37	506716.0
38	915199.0
39	463567.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.733391234860626	20.898419843665877	36.13552042725773	14.232668494215762
2	27.62402676840058	21.524527673176216	33.38719306662635	17.464252491796856
3	26.724434495940464	21.91368147269398	32.43983974749586	18.922044283869692
4	19.88266456040862	23.768618092118963	34.34907833212748	21.999639015344933
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	0.0	100.0	0.0	0.0
8	0.0	0.0	0.0	100.0
9	0.0	100.0	0.0	0.0
10	100.0	0.0	0.0	0.0
11	99.16078781044295	0.7124664662224808	0.08802471973552475	0.038721003599047864
12	98.79964888842952	0.9054544164312809	0.14291289933927467	0.1519837957999281
13	93.66120201719458	3.205358616657436	1.5777189163672605	1.5557204497807096
14	41.197327479416934	34.948744806988906	9.6902782512916	14.163649462302558
15	28.988456204385194	31.025674647924262	20.791451142886675	19.19441800480387
16	32.25694085559534	20.514017466103695	24.39747187327896	22.831569805022006
17	22.609086014621422	21.19020034648356	27.429804682618897	28.77090895627612
18	22.559010963751895	25.369816437760228	28.819441337679546	23.251731260808327
19	27.675922169240096	23.38622118486271	26.76118088262291	22.176675763274282
20	26.549804312378228	24.481732479517206	26.687194455028873	22.281268753075697
21	30.180877994899934	23.51469469799931	22.276023676890965	24.028403630209787
22	28.102192596112165	26.03371966918996	23.79006119769772	22.074026537000154
23	24.34844583764638	25.215580344539802	25.614576375251264	24.82139744256256
24	25.211384283592015	29.684817201381613	21.659449544681106	23.444348970345267
25	26.755411298819702	23.661340857446543	25.444851880591212	24.138395963142543
26	26.9110049411703	25.856189267031453	21.59641607029636	25.636389721501878
27	28.21199980870899	27.648740333247456	20.39044964186928	23.74881021617427
28	28.34053502862423	27.601472940806232	20.364810475342743	23.693181555226793
29	29.280668654653386	26.054915947654138	19.32045410018404	25.343961297508432
30	29.70422398326512	26.125261675308188	19.682888864548993	24.487625476877696
31	26.351386165648762	26.83871544998897	19.57530309598335	27.234595288378916
32	26.291407176806892	29.843002528435253	19.607760861550044	24.25782943320781
33	28.23387486173825	29.870400338153146	19.69029367798626	22.20543112212234
34	26.500469742852427	30.082116295680372	20.32636715224759	23.09104680921961
35	27.4788615716408	29.98866137942418	19.33257948218757	23.199897566747453
36	26.954477366724927	29.367520945594677	22.042864613784985	21.63513707389541
37	26.314115271347845	30.14046005488818	21.324505150202015	22.220919523561964
38	26.038316824198933	30.72655103844795	22.356242489128036	20.878889648225083
39	26.191226221678516	31.848287714026107	21.102823547923798	20.85766251637158
40	25.564161937099193	32.79823271785964	19.868256027595272	21.769349317445894
41	26.559399716457357	32.20809994029869	19.983154049430215	21.249346293813744
42	26.100393843514695	31.871797996689434	20.729250710013623	21.29855744978225
43	26.65760605466912	31.979723152537616	18.890296146257405	22.472374646535858
44	25.542595417963156	31.080100026688186	19.48795715081291	23.88934740453576
45	25.79269299180688	32.39834193885784	19.630222128976424	22.178742940358855
46	27.476455007273685	29.209952686327473	19.433099824598482	23.88049248180036
47	27.730069867500116	28.52698206029678	20.90051787413977	22.842430198063333
48	28.097348613988622	29.027177207984238	19.997562582243567	22.877911595783576
49	27.021953729172033	29.688858995382787	20.357683342409373	22.931503933035806
50	26.13084613877546	30.743088455124518	20.055968048230017	23.070097357870008
51	24.926638353539733	31.27225493538529	21.07943667881776	22.72167003225722
52	25.406531971053347	30.220432040010675	21.351902959919904	23.02113302901607
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	29.5
6	59.0
7	177.0
8	295.0
9	827.0
10	1359.0
11	2406.0
12	3453.0
13	4342.0
14	5218.0
15	5205.0
16	5137.0
17	5069.0
18	6103.0
19	7137.0
20	8755.5
21	10374.0
22	14311.0
23	18248.0
24	25229.5
25	32211.0
26	41074.0
27	49937.0
28	56895.5
29	63854.0
30	66196.0
31	68538.0
32	68630.0
33	68722.0
34	70692.5
35	72663.0
36	75497.5
37	78332.0
38	81804.5
39	89854.5
40	94432.0
41	106232.0
42	118032.0
43	128068.0
44	138104.0
45	148968.0
46	159832.0
47	173747.5
48	187663.0
49	198621.0
50	209579.0
51	213925.5
52	218272.0
53	228306.0
54	238340.0
55	235429.5
56	232519.0
57	240736.0
58	248953.0
59	233201.5
60	217450.0
61	201138.0
62	184826.0
63	158520.0
64	113010.0
65	93806.0
66	80301.0
67	66796.0
68	58474.0
69	50152.0
70	40886.5
71	31621.0
72	28455.0
73	25289.0
74	17090.0
75	8891.0
76	7126.0
77	5361.0
78	3404.0
79	1447.0
80	908.0
81	369.0
82	615.0
83	861.0
84	601.0
85	341.0
86	176.5
87	12.0
88	10.5
89	4.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	3241135.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.73266319672396
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.22346883189292	10.192858987609238
2	8.043924392372022	2.2092900892071485
3	3.781557138954584	1.5579255164529113
4	2.4313959478847473	1.335581666007226
5	1.6566609892071018	1.1375183697966338
6	1.180727865075836	0.9728722858844032
7	0.9006082241375337	0.8657424589996304
8	0.682233562781547	0.7495106991344014
9	0.5762493665708402	0.7122094621598586
>10	3.7792445735401454	9.909675595172525
>50	0.7804911070982316	7.909962150790858
>100	1.8694049634490224	53.641021135249446
>500	0.08606016107733556	7.477120344709505
>1k	0.00797287595811828	1.3287112388262152
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.04939627630444273	0.0	0.0	0.0	0.0
10	0.04939627630444273	0.0	0.0	0.0	0.0
11	0.06093544391085222	0.0	0.0	0.0	0.0
12	0.07623872501453965	0.0	0.0	0.0	0.0
13	0.08611180959756382	0.0	0.0	0.0	0.0
14	0.15713631181669385	0.0	0.0	0.0	0.0
15	0.21011158128248283	0.0	0.0	0.0	0.0
16	0.2419831324520577	0.0	0.0	0.0	0.0
17	0.2659253625658913	0.0	0.0	0.0	0.0
18	0.3141800634654218	0.0	0.0	0.0	0.0
19	0.3461750281922845	0.0	0.0	0.0	0.0
20	0.37928071493473736	0.0	0.0	0.0	0.0
21	0.41325029657820483	0.0	0.0	0.0	0.0
22	0.45119996544420393	0.0	0.0	0.0	0.0
23	0.4880697656839348	0.0	0.0	0.0	0.0
24	0.5353680115144849	0.0	0.0	0.0	0.0
25	0.5781925158933522	0.0	0.0	0.0	0.0
26	0.6250588142733949	0.0	0.0	0.0	0.0
27	0.6738380227913987	0.0	0.0	0.0	0.0
28	0.7264430515853243	0.0	0.0	0.0	0.0
29	0.7764563956762061	0.0	0.0	0.0	0.0
30	0.8351395421665558	0.0	0.0	0.0	0.0
31	0.8972782682609641	0.0	0.0	0.0	0.0
32	0.962193799394348	0.0	0.0	0.0	0.0
33	1.0197045170904637	0.0	0.0	0.0	0.0
34	1.0720318653804917	0.0	0.0	0.0	0.0
35	1.1218600891354418	0.0	0.0	0.0	0.0
36	1.1732618357458113	0.0	0.0	0.0	0.0
37	1.229569271258371	0.0	0.0	0.0	0.0
38	1.2903195948332915	0.0	0.0	0.0	0.0
39	1.359122653021241	0.0	0.0	0.0	0.0
40	1.4391563449223805	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATCCG	20	6.313162E-4	46.000004	46
CGACTAT	20	6.313162E-4	46.000004	28
GCGCTAA	40	5.6170393E-9	46.000004	34
ACCGGTA	50	1.6370905E-11	46.0	46
TTACTCG	25	3.4185425E-5	46.0	25
CGATTAG	25	3.4185425E-5	46.0	31
CGTTAAC	30	1.8624596E-6	46.0	33
TTACGCC	45	3.110472E-10	46.0	38
ACCGTAC	25	3.4185425E-5	46.0	31
ACGGTAG	25	3.4185425E-5	46.0	36
ACGAACT	45	3.110472E-10	46.0	28
TAAGCGC	35	1.0203439E-7	45.999996	39
TAGTCGG	35	1.0203439E-7	45.999996	19
GATTGCG	280	0.0	43.535713	13
CGCTATA	1240	0.0	43.403225	1
AGGGGAT	25065	0.0	42.760822	9
TATCCGG	70	0.0	42.714283	24
TCGCGTG	65	0.0	42.46154	20
CTATACA	22870	0.0	42.449932	3
TCATACA	25935	0.0	42.372856	3
>>END_MODULE