FastQCFastQC Report
Fri 17 Jun 2016
SRR1527937_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527937_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3343173
Sequences flagged as poor quality0
Sequence length59
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGAA203.139112E-453.00000444
TTACGCC600.053.038
TAAGGCG600.053.048
GCGCTAA307.0145506E-753.034
TATCTCG1150.050.6956519
GATTGCG2750.050.10909313
AGGGGAT258150.048.9246529
CGCTATA12400.048.7258031
TCATACA267500.048.5717743
CAGGGGG1397250.048.5506178
ACCATAC33050.048.509832
AGGGGGC354500.048.4923869
CTATACA239100.048.278553
CATACAG806050.048.2099154
CTCATAC41900.048.1933172
AACCGGT1100.048.18181615
CGGATTA551.0913936E-1148.18181643
AGGGGGG426200.048.0382469
CCGTATA6350.047.9921261
CCGATAC18450.047.97292
Produced by FastQC (version 0.11.5)