##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527937_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3343173
Sequences flagged as poor quality	0
Sequence length	59
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.34885182429985	31.0	31.0	34.0	30.0	34.0
2	31.947815144475022	33.0	31.0	34.0	30.0	34.0
3	32.16468426850779	34.0	31.0	34.0	30.0	34.0
4	35.78551992373712	37.0	35.0	37.0	35.0	37.0
5	35.759507210664836	37.0	35.0	37.0	35.0	37.0
6	35.73474660150701	37.0	35.0	37.0	33.0	37.0
7	35.863964862123495	37.0	35.0	37.0	35.0	37.0
8	35.881067776031934	37.0	35.0	37.0	35.0	37.0
9	37.371268552360284	39.0	37.0	39.0	34.0	39.0
10	37.34421610846941	39.0	37.0	39.0	34.0	39.0
11	37.78156021240899	39.0	37.0	39.0	35.0	39.0
12	37.78848267798286	39.0	37.0	39.0	35.0	39.0
13	37.61967567936209	39.0	37.0	39.0	35.0	39.0
14	37.77953040419984	40.0	38.0	40.0	33.0	40.0
15	36.823817672612215	39.0	35.0	40.0	31.0	40.0
16	36.770527878754706	39.0	36.0	40.0	31.0	40.0
17	36.69341490853151	39.0	35.0	40.0	31.0	40.0
18	36.80276820852525	39.0	35.0	40.0	31.0	40.0
19	36.91332814664392	39.0	35.0	40.0	31.0	40.0
20	36.96465902302992	39.0	35.0	40.0	32.0	40.0
21	36.97026088688799	39.0	35.0	40.0	32.0	40.0
22	36.94620619393612	39.0	35.0	40.0	31.0	40.0
23	36.885247936615905	39.0	35.0	40.0	31.0	40.0
24	36.91862909876336	39.0	35.0	40.0	31.0	40.0
25	36.87484524432328	39.0	35.0	40.0	31.0	40.0
26	36.72474472604319	39.0	35.0	40.0	31.0	40.0
27	36.63337643609828	39.0	35.0	40.0	31.0	40.0
28	36.578511791044015	39.0	35.0	40.0	31.0	40.0
29	36.54180654127082	39.0	35.0	40.0	30.0	40.0
30	36.48669452642744	39.0	35.0	40.0	30.0	40.0
31	36.47517971699341	39.0	35.0	40.0	30.0	40.0
32	36.365414831957544	39.0	35.0	40.0	30.0	40.0
33	36.23003655509302	38.0	35.0	40.0	30.0	40.0
34	36.16144782217373	38.0	35.0	40.0	30.0	40.0
35	36.03500746147447	38.0	35.0	40.0	30.0	40.0
36	35.80536484351842	38.0	35.0	40.0	29.0	40.0
37	35.70763044568738	38.0	35.0	40.0	29.0	40.0
38	35.53507610883433	38.0	34.0	40.0	28.0	40.0
39	35.373715927952276	38.0	34.0	40.0	28.0	40.0
40	35.17315167357477	38.0	34.0	40.0	27.0	40.0
41	35.031206282175646	37.0	34.0	40.0	27.0	40.0
42	34.86197872500167	37.0	34.0	40.0	26.0	40.0
43	34.619069668246304	37.0	33.0	40.0	26.0	40.0
44	34.43268086934179	36.0	33.0	40.0	25.0	40.0
45	34.18241413172456	36.0	33.0	40.0	24.0	40.0
46	33.92961447104293	36.0	33.0	39.0	23.0	40.0
47	33.75447965151669	35.0	33.0	39.0	23.0	40.0
48	33.53526485168431	35.0	33.0	39.0	23.0	40.0
49	33.38728208202208	35.0	33.0	39.0	23.0	40.0
50	33.11261068452036	35.0	32.0	39.0	22.0	40.0
51	32.89695328360214	35.0	32.0	38.0	21.0	40.0
52	32.703805037908594	35.0	32.0	38.0	20.0	40.0
53	32.44995517731209	35.0	31.0	38.0	20.0	40.0
54	32.19074872882737	35.0	31.0	38.0	19.0	40.0
55	31.945795805362152	35.0	31.0	38.0	18.0	40.0
56	31.61409774486693	35.0	31.0	37.0	17.0	40.0
57	31.321599570228642	35.0	30.0	37.0	15.0	39.0
58	31.036643332546657	34.0	30.0	36.0	13.0	39.0
59	30.450072730307404	34.0	29.0	36.0	10.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	6.0
13	5.0
14	23.0
15	96.0
16	328.0
17	897.0
18	2013.0
19	3833.0
20	6580.0
21	10453.0
22	15552.0
23	21564.0
24	28946.0
25	38175.0
26	47993.0
27	60093.0
28	69597.0
29	80995.0
30	95602.0
31	116204.0
32	142330.0
33	181962.0
34	269434.0
35	344104.0
36	353424.0
37	541148.0
38	745362.0
39	166452.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.895603069299735	21.13985725536788	35.65597712113612	14.30856255419627
2	27.278965222559528	21.947174136665975	33.26567305969508	17.50818758107941
3	26.642952668019277	22.112376475880847	32.298328563912186	18.94634229218769
4	20.00156737327084	23.965735545243994	34.16751092450197	21.865186156983203
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	0.0	100.0	0.0	0.0
8	0.0	0.0	0.0	100.0
9	0.0	100.0	0.0	0.0
10	100.0	0.0	0.0	0.0
11	99.15209293685969	0.7251195196898276	0.08411171064135778	0.03867583280913073
12	98.794109667672	0.9076108236097863	0.13633754520032318	0.16194196351789153
13	93.62432036870362	3.2442832004206776	1.5551393840522163	1.576257046823482
14	40.95567294902178	35.15734902142366	9.778435037612471	14.108542991942086
15	28.765846098900656	31.38712833586536	20.797428072074045	19.049597493159943
16	32.357733207345234	20.71840135105183	24.199944184761005	22.723921256841926
17	22.553663839711554	21.31956677084913	27.331968761413183	28.79480062802613
18	22.555189336597298	25.594846572402925	28.708086599167913	23.14187749183186
19	27.69689154584582	23.563841895109825	26.743844844403803	21.995421714640553
20	26.48008344168848	24.67278839593404	26.755630055638758	22.09149810673872
21	30.133528836228336	23.64897060367501	22.398362274402192	23.819138285694457
22	28.044226248536948	26.34374589648816	23.785637177615396	21.826390677359502
23	24.245918473258786	25.406043899014495	25.757955092362856	24.590082535363862
24	25.070793524594748	30.091802009647722	21.61898292430574	23.21842154145179
25	26.649054655562242	23.803105612542335	25.547436522130322	24.000403209765096
26	26.786528845500968	26.095747961592174	21.548989537783417	25.568733655123445
27	28.15540805097433	27.860987152025935	20.404657491550694	23.578947305449045
28	28.191481565566605	27.903072919050253	20.345910905597766	23.559534609785374
29	29.222508078403358	26.242614426474493	19.271422687369157	25.263454807752993
30	29.737258586378868	26.308330439376004	19.689917333024646	24.26449364122048
31	26.274231097224103	27.082086389187754	19.449337500631884	27.194345012956255
32	26.167057463074748	30.24758216221536	19.53446620919707	24.050894165512823
33	28.160672510815328	30.335971246477524	19.60110948491149	21.902246757795663
34	26.39369844157033	30.501592349543383	20.30140229057844	22.803306918307847
35	27.334271962593622	30.380449949793203	19.321315409044043	22.963962678569132
36	26.614028050597444	29.980889412543117	22.04190450210025	21.363178034759194
37	26.149409557925956	30.56512480807903	21.281967759371113	22.003497874623896
38	25.842724860484335	31.11986726382392	22.328039859139807	20.70936801655194
39	25.90449252850511	32.373706057090075	21.042763865345883	20.679037549058933
40	25.335302719901126	33.3692572894074	19.790360833854546	21.505079156836935
41	26.295677788735432	32.786158538609875	19.934535245409077	20.983628427245613
42	25.64306423867386	32.45692041662217	20.718760291495535	21.181255053208435
43	26.429054075275193	32.532776497058336	18.747728580004683	22.290440847661788
44	25.095739885432195	31.54374601613497	19.482838608710946	23.877675489721888
45	25.38591332246342	33.05407766813144	19.535662677342753	22.024346332062386
46	27.380934220275172	29.62105161772962	19.291972027771223	23.706042134223985
47	27.56225896775309	28.980522395939428	20.825335691572047	22.631882944735434
48	27.823178758622426	29.480945197870405	19.94410699057452	22.751769052932648
49	26.876563073463444	30.183690763236005	20.17275205321412	22.766994110086436
50	25.798036775243162	31.33328726930972	19.86451194718311	23.004164008264006
51	24.689718420195426	31.784116466602235	21.00223350691095	22.523931606291388
52	25.191726542419435	30.704692817272694	21.2924966790531	22.81108396125477
53	25.108930946738322	30.974107531976358	20.45362295041268	23.46333857087264
54	23.71752822842252	31.350486498903884	21.53523015410809	23.39675511856551
55	24.595825582463128	30.24276637792899	21.28588619254822	23.875521847059662
56	24.917196926392982	30.929120329698762	21.080602170453037	23.07308057345522
57	25.12948028714039	29.571308454572947	22.160923170891845	23.13828808739482
58	25.166780181581988	28.56232686732036	22.12990473421507	24.140988216882583
59	25.473434967319967	28.589696076152805	21.82205348033141	24.114815476195815
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	18.5
5	36.0
6	131.0
7	226.0
8	633.5
9	1795.0
10	2549.0
11	3095.0
12	3641.0
13	3617.0
14	3417.0
15	3241.0
16	3444.5
17	3648.0
18	4114.5
19	5806.0
20	7031.0
21	10432.5
22	13834.0
23	19834.5
24	25835.0
25	32350.0
26	44853.5
27	50842.0
28	57372.5
29	63903.0
30	65767.5
31	68349.5
32	69067.0
33	69638.0
34	70209.0
35	73894.5
36	80627.0
37	83674.0
38	86328.0
39	88982.0
40	92767.0
41	96552.0
42	102266.5
43	113687.5
44	119394.0
45	126603.0
46	133812.0
47	141605.0
48	161559.0
49	173720.0
50	183092.0
51	192464.0
52	191279.0
53	202173.5
54	214253.0
55	220115.5
56	225978.0
57	224286.5
58	211171.0
59	199747.0
60	182736.0
61	165725.0
62	148322.5
63	130920.0
64	115527.5
65	85408.5
66	70682.0
67	63507.5
68	56333.0
69	50493.5
70	36884.5
71	29115.0
72	23480.0
73	17845.0
74	14691.0
75	8490.5
76	5444.0
77	3526.0
78	1608.0
79	1560.5
80	1513.0
81	1413.5
82	739.0
83	164.0
84	150.5
85	137.0
86	86.0
87	23.0
88	11.0
89	6.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	3343173.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.73644197488689
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.15745001323933	15.39338294977729
2	6.395593989855924	2.3966135137174174
3	2.7058901230567916	1.520962598432194
4	1.6216916741688692	1.215389278168888
5	1.0379495384452047	0.9723740649969604
6	0.7149307995437331	0.8037155665026388
7	0.5274522759505172	0.6917805274008226
8	0.417535479645472	0.6258503429467163
9	0.3123467287397553	0.5267039723170345
>10	2.198651335106999	8.121398904812668
>50	0.530861136842955	7.338095169385027
>100	1.311008637803102	51.61059204850926
>500	0.06274094628012873	7.42979438444193
>1k	0.005897321321369803	1.3533466785911317
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.04447870331568244	0.0	0.0	0.0	0.0
10	0.04447870331568244	0.0	0.0	0.0	0.0
11	0.056413473068848065	0.0	0.0	0.0	0.0
12	0.07142914829714167	0.0	0.0	0.0	0.0
13	0.0804026593897474	0.0	0.0	0.0	0.0
14	0.14492220414558266	0.0	0.0	0.0	0.0
15	0.19526360137510085	0.0	0.0	0.0	0.0
16	0.22463689435156362	0.0	0.0	0.0	0.0
17	0.2463228794920275	0.0	0.0	0.0	0.0
18	0.2896649380693132	0.0	0.0	0.0	0.0
19	0.3165256479398464	0.0	0.0	0.0	0.0
20	0.34622796965637137	0.0	0.0	0.0	0.0
21	0.3772464063331452	0.0	0.0	0.0	0.0
22	0.411345748485047	0.0	0.0	0.0	0.0
23	0.44634244174620935	0.0	0.0	0.0	0.0
24	0.48935547158343284	0.0	0.0	0.0	0.0
25	0.528659450169046	0.0	0.0	0.0	0.0
26	0.5743346216304092	0.0	0.0	0.0	0.0
27	0.6222830825685659	0.0	0.0	0.0	0.0
28	0.6725048329835159	0.0	0.0	0.0	0.0
29	0.7183594746667312	0.0	0.0	0.0	0.0
30	0.7733670976644045	0.0	0.0	0.0	0.0
31	0.829032778142202	0.0	0.0	0.0	0.0
32	0.8900526535719211	0.0	0.0	0.0	0.0
33	0.9429365456110108	0.0	0.0	0.0	0.0
34	0.993158296025961	0.0	0.0	0.0	0.0
35	1.0410170218531916	0.0	0.0	0.0	0.0
36	1.0905208913807332	0.0	0.0	0.0	0.0
37	1.143434695123465	0.0	0.0	0.0	0.0
38	1.199788344785029	0.0	0.0	0.0	0.0
39	1.265265064057409	0.0	0.0	0.0	0.0
40	1.343394433970363	0.0	0.0	0.0	0.0
41	1.4224510666962193	0.0	0.0	0.0	0.0
42	1.5017469930512122	0.0	0.0	0.0	0.0
43	1.582807709921084	0.0	0.0	0.0	0.0
44	1.6654537470839827	0.0	0.0	0.0	0.0
45	1.7496851045399087	0.0	0.0	0.0	2.9911703642019124E-5
46	1.8328994640720058	0.0	0.0	0.0	2.9911703642019124E-5
47	1.9099819243574891	0.0	0.0	0.0	2.9911703642019124E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACGAA	20	3.139112E-4	53.000004	44
TTACGCC	60	0.0	53.0	38
TAAGGCG	60	0.0	53.0	48
GCGCTAA	30	7.0145506E-7	53.0	34
TATCTCG	115	0.0	50.69565	19
GATTGCG	275	0.0	50.109093	13
AGGGGAT	25815	0.0	48.924652	9
CGCTATA	1240	0.0	48.725803	1
TCATACA	26750	0.0	48.571774	3
CAGGGGG	139725	0.0	48.550617	8
ACCATAC	3305	0.0	48.50983	2
AGGGGGC	35450	0.0	48.492386	9
CTATACA	23910	0.0	48.27855	3
CATACAG	80605	0.0	48.209915	4
CTCATAC	4190	0.0	48.193317	2
AACCGGT	110	0.0	48.181816	15
CGGATTA	55	1.0913936E-11	48.181816	43
AGGGGGG	42620	0.0	48.038246	9
CCGTATA	635	0.0	47.992126	1
CCGATAC	1845	0.0	47.9729	2
>>END_MODULE