FastQCFastQC Report
Fri 17 Jun 2016
SRR1527936_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527936_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1316630
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATATCTCTGGGGGAGGCCATGGCCATGGGCAGCGTGGAGGCCTCTTATAA23670.17977715835124522No Hit
GTCGATCTCTGGGTTGATACCACCCAAAAAAAAAAAAAAAAAAAAAAAAAAA17270.13116820974761323No Hit
TGTTATCTCTGGGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATT17020.12926942269278385No Hit
TTTTATCTCTGGGGTGGCAGTCCTCCTGCCTCAGCTTCCCAAGTGCTGAGTT16530.12554780006531827No Hit
TGTAATCTCTGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATC15190.11537030145143282No Hit
TAGCATCTCTGGGCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCC14560.1105853580732628No Hit
GACGATCTCTGGGTGGCTAACACGGTGAAACCCCGTCTCTACCAAAAAATAC14110.10716754137456992No Hit
GGGCATCTCTGGGGGGAATTCGTGGAGAAAGAAATGGCCCGGTGGGCTGCAA13820.10496494839096786No Hit
CAAGATCTCTGGGGCGATCCTGCCCAAAATCCGTGAAATTGTGCTAATGGGC13450.10215474354982038No Hit
TGCGATCTCTGGGATGTACATCTTCCCTGTCCACTGGCAGTTTGGCCAGCTG13430.10200284058543402No Hit
GTCGATCTCTGGGTTGATACCACCGAAAAAAAAAAAAAAAAAAAAAAAAAAA13210.10033190797718418No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTAGT1450.046.00000442
TGATCGT405.6115823E-946.035
TCACGGG253.41743E-546.032
TCACGAA501.6370905E-1146.041
TGTTTTA501.6370905E-1146.029
CTTAAGT206.3117914E-446.025
AACGTTA405.6115823E-946.043
TGTCATA850.046.024
TTTAGGG453.110472E-1046.028
GTGTTAC405.6115823E-946.040
ACAACCC206.3117914E-446.028
GTCATCG206.3117914E-446.031
GTCATAG850.046.025
TTAGGTA405.6115823E-946.024
CTTCGAA453.110472E-1046.026
GTGCAAT206.3117914E-446.022
AGCAATT253.41743E-546.044
AACCGTG850.046.021
AACGTAC405.6115823E-946.015
CTAGAAG301.861612E-646.043
Produced by FastQC (version 0.11.5)