##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527936_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1316630
Sequences flagged as poor quality	0
Sequence length	52
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.926894419844604	33.0	31.0	34.0	30.0	34.0
2	32.40857264379515	34.0	31.0	34.0	30.0	34.0
3	32.5549972277709	34.0	31.0	34.0	31.0	34.0
4	36.050500900025064	37.0	35.0	37.0	35.0	37.0
5	35.993557795280374	37.0	35.0	37.0	35.0	37.0
6	36.09516416912876	37.0	36.0	37.0	35.0	37.0
7	35.95914873578758	37.0	35.0	37.0	35.0	37.0
8	36.08876297820952	37.0	36.0	37.0	35.0	37.0
9	37.7355589649332	39.0	38.0	39.0	35.0	39.0
10	38.01178463197709	39.0	38.0	39.0	35.0	39.0
11	38.18544617698215	39.0	39.0	39.0	37.0	39.0
12	38.09807159186712	39.0	39.0	39.0	37.0	39.0
13	38.10750476595551	39.0	39.0	39.0	37.0	39.0
14	38.658101364848136	40.0	39.0	40.0	36.0	40.0
15	38.13009121773011	40.0	38.0	40.0	34.0	40.0
16	37.67751912078564	40.0	37.0	40.0	33.0	40.0
17	37.62325026772898	40.0	37.0	40.0	33.0	40.0
18	37.69394742638403	40.0	37.0	40.0	33.0	40.0
19	37.854780006531826	40.0	38.0	40.0	33.0	40.0
20	37.92835496684718	40.0	38.0	40.0	34.0	40.0
21	37.77036145310376	40.0	37.0	40.0	33.0	40.0
22	37.84645344553899	40.0	37.0	40.0	33.0	40.0
23	37.7718242786508	40.0	37.0	40.0	33.0	40.0
24	37.75568458868475	40.0	37.0	40.0	33.0	40.0
25	37.833892589413885	40.0	37.0	40.0	33.0	40.0
26	37.788274610179016	40.0	37.0	40.0	33.0	40.0
27	37.76397317393649	40.0	37.0	40.0	33.0	40.0
28	37.709282030638825	40.0	37.0	40.0	33.0	40.0
29	37.668213545187335	40.0	37.0	40.0	33.0	40.0
30	37.631355809908634	40.0	37.0	40.0	33.0	40.0
31	37.56601399026302	40.0	37.0	40.0	33.0	40.0
32	37.51428951185982	40.0	37.0	40.0	33.0	40.0
33	37.447005612814536	40.0	36.0	40.0	33.0	40.0
34	37.36595398859209	40.0	36.0	40.0	33.0	40.0
35	37.26165133712584	40.0	36.0	40.0	33.0	40.0
36	37.188617910878534	40.0	35.0	40.0	33.0	40.0
37	37.05363162012107	39.0	35.0	40.0	32.0	40.0
38	36.853623265458026	39.0	35.0	40.0	32.0	40.0
39	36.699289853641496	39.0	35.0	40.0	32.0	40.0
40	36.504203914539396	39.0	35.0	40.0	31.0	40.0
41	36.32898384511974	39.0	35.0	40.0	31.0	40.0
42	36.15605599143267	38.0	35.0	40.0	31.0	40.0
43	35.938568922172514	38.0	35.0	40.0	30.0	40.0
44	35.757457296279135	38.0	35.0	40.0	30.0	40.0
45	35.56420102838307	38.0	35.0	40.0	30.0	40.0
46	35.36883482831168	37.0	35.0	40.0	29.0	40.0
47	35.180854150368745	37.0	34.0	40.0	28.0	40.0
48	34.95056925635904	37.0	34.0	40.0	28.0	40.0
49	34.7477939891997	36.0	34.0	40.0	27.0	40.0
50	34.54254346323569	36.0	34.0	40.0	27.0	40.0
51	34.285278324206494	35.0	34.0	39.0	26.0	40.0
52	33.60890303274268	35.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	7.0
15	6.0
16	26.0
17	47.0
18	127.0
19	264.0
20	544.0
21	969.0
22	1593.0
23	2448.0
24	3745.0
25	5535.0
26	7820.0
27	10631.0
28	14246.0
29	18714.0
30	23928.0
31	30018.0
32	36820.0
33	46709.0
34	66502.0
35	112584.0
36	114143.0
37	217275.0
38	399527.0
39	202399.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.285623143935656	21.175577041385964	33.60139143115378	16.937408383524605
2	20.128813713799627	24.238776269718905	34.26505548255774	21.36735453392373
3	22.396573069123445	22.132641668502163	32.73660785490229	22.734177407472107
4	21.798531098334383	18.363321510219272	34.39599583785878	25.442151553587568
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	0.0	0.0	0.0	100.0
8	0.0	0.0	100.0	0.0
9	0.0	0.0	0.0	100.0
10	0.0	0.0	100.0	0.0
11	99.38889437427372	0.30532495841656354	0.25793123352802233	0.04784943378170025
12	99.4413768484692	0.20742349786956094	0.24479162710860305	0.10640802655263817
13	98.42180415150801	0.8712394522379103	0.23750028481805824	0.46945611143601473
14	63.888260179397406	17.330229449427705	10.169751562701746	8.611758808473146
15	32.032081906078396	25.692335735931888	18.91184311461838	23.363739243371334
16	30.410593712736304	15.938114732309	30.312920106635882	23.338371448318814
17	21.784707928575227	18.399322512778838	31.389988075617296	28.42598148302864
18	22.888510819288637	16.685781123018618	36.0048760851568	24.420831972535943
19	22.04218345321009	19.22134540455557	30.723665722336573	28.012805419897767
20	23.840486697097894	17.618009615457645	27.83128137745608	30.710222309988378
21	28.60963216697174	17.431093017780242	22.302393231203908	31.65688158404411
22	27.76740618093162	21.477939891996993	22.407813888488036	28.346840038583355
23	27.728215216119946	18.36446078245217	21.966991485838847	31.94033251558904
24	26.53547313975832	21.515687778647	20.61915648283876	31.329682598755916
25	31.301580550344436	17.639959593811476	23.732559640901393	27.325900214942695
26	29.628597252075373	19.02926410608903	20.565686639374768	30.77645200246083
27	29.731967219340284	21.648071212109706	20.595383668912298	28.02457789963771
28	29.923744711878054	22.886308226305037	19.981012129451706	27.208934932365203
29	29.988835132117604	23.144619217244024	19.563810637764593	27.302735012873775
30	31.12765165612207	21.77377091513941	18.51355354199737	28.58502388674115
31	28.89118431146184	21.385203132239123	20.572294418325573	29.151318137973465
32	28.89141216590842	24.362956943104745	18.57188428032173	28.173746610665106
33	30.616346277997614	23.135808845309615	19.142279911592475	27.105564965100292
34	29.383425867555808	23.967097817913917	19.51436622285684	27.135110091673436
35	29.103240849745184	25.020924633344222	20.025215892088134	25.850618624822463
36	30.957444384527165	24.504074797019666	19.77844952644251	24.760031292010666
37	30.24388020932228	24.38984376780113	19.32805723703698	26.038218785839607
38	29.791816987308508	26.223160644979988	20.13754813425184	23.847474233459668
39	30.923190266058043	23.53637696239642	20.329325626789608	25.21110714475593
40	30.6910825364757	25.664309639002603	19.58545681018965	24.059151014332045
41	30.07063487843965	26.009964834463744	19.346437495727727	24.572962791368873
42	31.48219317499981	24.80150080128814	18.370916658438592	25.345389365273462
43	30.183119023567745	25.9425958697584	17.36547093716534	26.508814169508515
44	30.022785444657952	25.46045586079612	19.899819994987205	24.616938699558723
45	27.825129307398434	25.452708809612417	20.137016473876486	26.58514540911266
46	28.991212413510247	23.943476906951837	20.47340558851006	26.59190509102785
47	29.953973401790932	22.758481881773925	21.947775760843975	25.33976895559117
48	29.570342465233207	22.258645177460636	21.73404828995238	26.436964067353774
49	28.703812004891276	21.60371554650889	22.069981695692793	27.62249075290704
50	28.501401304846464	23.79711840076559	21.00855973204317	26.692920562344774
51	28.150505457113994	24.207712113501895	21.91686350759135	25.72491892179276
52	29.20820579813615	24.425237158503148	20.076635045532914	26.289921997827786
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	12.0
8	24.0
9	22.5
10	21.0
11	79.0
12	137.0
13	233.0
14	431.5
15	534.0
16	612.0
17	690.0
18	943.5
19	1197.0
20	1933.0
21	2669.0
22	4463.0
23	6257.0
24	8595.5
25	10934.0
26	14347.5
27	17761.0
28	16888.0
29	16015.0
30	14890.5
31	13766.0
32	12728.0
33	11690.0
34	13073.5
35	14457.0
36	13785.0
37	13113.0
38	13348.0
39	17397.0
40	21211.0
41	24229.0
42	27247.0
43	28746.5
44	30246.0
45	36482.0
46	42718.0
47	44306.0
48	45894.0
49	55854.5
50	65815.0
51	72017.0
52	78219.0
53	88211.0
54	98203.0
55	105810.0
56	113417.0
57	114598.0
58	115779.0
59	118202.5
60	120626.0
61	111169.0
62	101712.0
63	95859.0
64	84264.0
65	78522.0
66	67742.0
67	56962.0
68	50206.5
69	43451.0
70	36998.0
71	30545.0
72	24019.5
73	17494.0
74	13894.0
75	10294.0
76	6711.5
77	3129.0
78	1954.5
79	780.0
80	787.0
81	794.0
82	427.5
83	61.0
84	178.5
85	296.0
86	163.0
87	30.0
88	16.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1316630.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.869532522039364
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.42221477996036	8.306737489570564
2	8.007896874013824	1.7408419101046124
3	3.71172718492231	1.21034218048352
4	2.2329842180259325	0.9708597831613405
5	1.4479095878873207	0.7869050177256923
6	1.0249077958832928	0.6684161171667081
7	0.6762867670482406	0.5145644706058997
8	0.5084891200477731	0.44216312219699583
9	0.3972774192882434	0.38863978463028875
>10	2.6969310815034078	5.678684562779897
>50	0.482293948518133	3.8447680550249954
>100	2.132357938564865	55.71772879041659
>500	0.2383347646467159	16.8526503407282
>1k	0.020388519689542067	2.8766983754046933
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAATATCTCTGGGGGAGGCCATGGCCATGGGCAGCGTGGAGGCCTCTTATAA	2367	0.17977715835124522	No Hit
GTCGATCTCTGGGTTGATACCACCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	1727	0.13116820974761323	No Hit
TGTTATCTCTGGGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATT	1702	0.12926942269278385	No Hit
TTTTATCTCTGGGGTGGCAGTCCTCCTGCCTCAGCTTCCCAAGTGCTGAGTT	1653	0.12554780006531827	No Hit
TGTAATCTCTGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATC	1519	0.11537030145143282	No Hit
TAGCATCTCTGGGCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCC	1456	0.1105853580732628	No Hit
GACGATCTCTGGGTGGCTAACACGGTGAAACCCCGTCTCTACCAAAAAATAC	1411	0.10716754137456992	No Hit
GGGCATCTCTGGGGGGAATTCGTGGAGAAAGAAATGGCCCGGTGGGCTGCAA	1382	0.10496494839096786	No Hit
CAAGATCTCTGGGGCGATCCTGCCCAAAATCCGTGAAATTGTGCTAATGGGC	1345	0.10215474354982038	No Hit
TGCGATCTCTGGGATGTACATCTTCCCTGTCCACTGGCAGTTTGGCCAGCTG	1343	0.10200284058543402	No Hit
GTCGATCTCTGGGTTGATACCACCGAAAAAAAAAAAAAAAAAAAAAAAAAAA	1321	0.10033190797718418	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.002962107805533825	0.0	0.0	0.0	0.0
12	0.009493935274146876	0.0	0.0	0.0	0.0
13	0.01260794604406705	0.0	0.0	0.0	0.0
14	0.03987452815141687	0.0	0.0	0.0	0.0
15	0.0625840213271762	0.0	0.0	0.0	0.0
16	0.07974905630283374	0.0	0.0	0.0	0.0
17	0.0953950616346278	0.0	0.0	0.0	0.0
18	0.12554780006531827	0.0	0.0	0.0	0.0
19	0.15038393474248649	0.0	0.0	0.0	0.0
20	0.1768910020279046	0.0	0.0	0.0	0.0
21	0.20560066229692472	0.0	0.0	0.0	0.0
22	0.24433591821544398	0.0	0.0	0.0	0.0
23	0.27395699627078224	0.0	0.0	0.0	0.0
24	0.30547686138094987	0.0	0.0	0.0	0.0
25	0.3314522682910157	0.0	0.0	0.0	0.0
26	0.37558007944525035	0.0	0.0	0.0	0.0
27	0.4168976857583376	0.0	0.0	0.0	0.0
28	0.4586710009645838	0.0	0.0	0.0	0.0
29	0.5087230277298862	0.0	0.0	0.0	0.0
30	0.5773831676325164	0.0	0.0	0.0	0.0
31	0.6385241107980222	0.0	0.0	0.0	0.0
32	0.692677517601756	0.0	0.0	0.0	0.0
33	0.7354382020765136	0.0	0.0	0.0	0.0
34	0.7810850428746117	0.0	0.0	0.0	0.0
35	0.8236938243849828	0.0	0.0	0.0	0.0
36	0.8695685196296605	0.0	0.0	0.0	0.0
37	0.9173420019291676	0.0	0.0	0.0	0.0
38	0.9677737861054359	0.0	0.0	0.0	0.0
39	1.0150156080295907	0.0	0.0	0.0	0.0
40	1.069852578173063	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGTAGT	145	0.0	46.000004	42
TGATCGT	40	5.6115823E-9	46.0	35
TCACGGG	25	3.41743E-5	46.0	32
TCACGAA	50	1.6370905E-11	46.0	41
TGTTTTA	50	1.6370905E-11	46.0	29
CTTAAGT	20	6.3117914E-4	46.0	25
AACGTTA	40	5.6115823E-9	46.0	43
TGTCATA	85	0.0	46.0	24
TTTAGGG	45	3.110472E-10	46.0	28
GTGTTAC	40	5.6115823E-9	46.0	40
ACAACCC	20	6.3117914E-4	46.0	28
GTCATCG	20	6.3117914E-4	46.0	31
GTCATAG	85	0.0	46.0	25
TTAGGTA	40	5.6115823E-9	46.0	24
CTTCGAA	45	3.110472E-10	46.0	26
GTGCAAT	20	6.3117914E-4	46.0	22
AGCAATT	25	3.41743E-5	46.0	44
AACCGTG	85	0.0	46.0	21
AACGTAC	40	5.6115823E-9	46.0	15
CTAGAAG	30	1.861612E-6	46.0	43
>>END_MODULE