FastQCFastQC Report
Fri 17 Jun 2016
SRR1527935_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527935_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1306754
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATATCTCTGGGGGAGGCCATGGCCATGGGCAGCGTGGAGGCCTCTTATAA22830.17470771086218218No Hit
TTTTATCTCTGGGGTGGCAGTCCTCCTGCCTCAGCTTCCCAAGTGCTGAGTT16570.1268027494080753No Hit
GTCGATCTCTGGGTTGATACCACCCAAAAAAAAAAAAAAAAAAAAAAAAAAA16150.12358867851179334No Hit
TGTTATCTCTGGGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATT15510.11869104666983993No Hit
TAGCATCTCTGGGCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCC15490.11853799567477886No Hit
TGTAATCTCTGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATC14700.11249248136986761No Hit
GGGCATCTCTGGGGGGAATTCGTGGAGAAAGAAATGGCCCGGTGGGCTGCAA13770.10537561009952906No Hit
GACGATCTCTGGGTGGCTAACACGGTGAAACCCCGTCTCTACCAAAAAATAC13360.10223806470077766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGCCC1150.046.00000419
CCGTAAA750.046.00000419
CGTAAAC750.046.00000420
TCACGGT600.046.027
CGGACAA301.861601E-646.024
TCACGAA551.8189894E-1246.041
TATTTCG206.3117745E-446.037
AACGTTT301.861601E-646.029
TTTAGCG253.4174136E-546.016
ATCCTAG405.6115823E-946.036
CTATGTG206.3117745E-446.044
AACGTGT206.3117745E-446.014
ACTATTC301.861601E-646.043
AGCAATT453.110472E-1046.044
AACGTAC600.046.015
AACGTAA301.861601E-646.016
GAAATAG206.3117745E-446.014
CGGGTAC301.861601E-646.046
TCGTTTA600.046.022
ACTATAG551.8189894E-1246.014
Produced by FastQC (version 0.11.5)