##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527935_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1306754
Sequences flagged as poor quality	0
Sequence length	52
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.87847750992153	33.0	31.0	34.0	30.0	34.0
2	32.253291744276275	34.0	31.0	34.0	30.0	34.0
3	32.486722060923476	34.0	31.0	34.0	30.0	34.0
4	36.016154532528695	37.0	35.0	37.0	35.0	37.0
5	35.974465737238994	37.0	35.0	37.0	35.0	37.0
6	36.08308526317884	37.0	35.0	37.0	35.0	37.0
7	35.95715720020754	37.0	35.0	37.0	35.0	37.0
8	36.03893693839851	37.0	36.0	37.0	35.0	37.0
9	37.72784548583743	39.0	38.0	39.0	35.0	39.0
10	38.00385611982056	39.0	38.0	39.0	35.0	39.0
11	38.175589284593734	39.0	39.0	39.0	37.0	39.0
12	38.07684384360025	39.0	39.0	39.0	37.0	39.0
13	38.11022656138799	39.0	39.0	39.0	37.0	39.0
14	38.687443849416184	40.0	39.0	40.0	36.0	40.0
15	38.149663211285365	40.0	38.0	40.0	34.0	40.0
16	37.71002652373745	40.0	37.0	40.0	33.0	40.0
17	37.66778521435557	40.0	37.0	40.0	33.0	40.0
18	37.76445911013091	40.0	37.0	40.0	33.0	40.0
19	37.91520515720633	40.0	38.0	40.0	33.0	40.0
20	37.96368023361704	40.0	38.0	40.0	34.0	40.0
21	37.990981470116026	40.0	38.0	40.0	34.0	40.0
22	37.95922338864086	40.0	38.0	40.0	34.0	40.0
23	37.857873784966415	40.0	37.0	40.0	34.0	40.0
24	37.84866700235852	40.0	37.0	40.0	33.0	40.0
25	37.81580389269901	40.0	37.0	40.0	33.0	40.0
26	37.75283947858587	40.0	37.0	40.0	33.0	40.0
27	37.73824453569685	40.0	37.0	40.0	33.0	40.0
28	37.53734367753992	40.0	37.0	40.0	33.0	40.0
29	37.59759679327555	40.0	37.0	40.0	33.0	40.0
30	37.62191353537085	40.0	37.0	40.0	33.0	40.0
31	37.591185487092446	40.0	37.0	40.0	33.0	40.0
32	37.540865380936275	40.0	37.0	40.0	33.0	40.0
33	37.467097097081776	40.0	37.0	40.0	33.0	40.0
34	37.412353817168345	40.0	36.0	40.0	33.0	40.0
35	37.23977504564746	40.0	36.0	40.0	33.0	40.0
36	36.79255468129426	40.0	35.0	40.0	31.0	40.0
37	36.88164643077427	39.0	35.0	40.0	32.0	40.0
38	36.78320479600598	39.0	35.0	40.0	32.0	40.0
39	36.677395286335454	39.0	35.0	40.0	31.0	40.0
40	36.490090713324776	39.0	35.0	40.0	31.0	40.0
41	36.34801806613946	39.0	35.0	40.0	31.0	40.0
42	36.026889529322276	38.0	35.0	40.0	30.0	40.0
43	35.81894985590249	38.0	35.0	40.0	30.0	40.0
44	35.64405924910121	38.0	35.0	40.0	30.0	40.0
45	35.22135918466674	37.0	35.0	40.0	28.0	40.0
46	35.18072338022306	37.0	34.0	40.0	28.0	40.0
47	35.06413984575521	37.0	34.0	40.0	28.0	40.0
48	34.83249716473031	37.0	34.0	40.0	27.0	40.0
49	34.63447749155541	36.0	34.0	40.0	27.0	40.0
50	34.37453338577881	36.0	34.0	40.0	26.0	40.0
51	34.21557003077856	35.0	34.0	39.0	26.0	40.0
52	33.66818773847258	35.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	7.0
15	3.0
16	24.0
17	50.0
18	122.0
19	291.0
20	511.0
21	994.0
22	1693.0
23	2457.0
24	3874.0
25	5571.0
26	7778.0
27	10953.0
28	14337.0
29	18978.0
30	24234.0
31	30462.0
32	37127.0
33	47127.0
34	67006.0
35	112561.0
36	115074.0
37	219148.0
38	386186.0
39	200184.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.351931580083168	21.171850248784395	33.56071609499569	16.91550207613675
2	20.271374719342738	24.34321991744429	34.09417533828096	21.291230024932005
3	22.426944933782487	22.108904966045635	32.72873088584385	22.735419214328022
4	21.808771964730926	18.37009873319691	34.36507559953901	25.45605370253315
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	0.0	0.0	0.0	100.0
8	0.0	0.0	100.0	0.0
9	0.0	0.0	0.0	100.0
10	0.0	0.0	100.0	0.0
11	99.39085703965705	0.3021991897480321	0.2555186362544136	0.05142513434051091
12	99.43677233817536	0.20677189432747098	0.2499322749346855	0.1065234925624869
13	98.4189832210194	0.8647381220949009	0.24335108214706058	0.4729275747386272
14	63.908432650674875	17.31588347921644	10.196563392956898	8.579120477151783
15	32.09464061330595	25.755038821384897	18.905853741408098	23.244466823901057
16	30.565661172646113	15.958321153024976	30.184028516461396	23.29198915786751
17	21.71977281110293	18.461087549760705	31.374841783533856	28.444297855602507
18	22.882501220581688	16.737274192388163	35.93002202403819	24.45020256299196
19	22.053959658818723	19.25733535156579	30.693764855512207	27.99494013410328
20	23.844809352028	17.656651519719855	27.83163472237315	30.666904405879
21	28.239439098713298	17.57836593574613	22.3377162036619	31.84447876187867
22	27.727713096726696	21.46739172024727	22.474620318744	28.33027486428203
23	27.658304470466515	18.370481360684565	21.988300781937532	31.98291338691138
24	26.597508023698417	21.37517849572299	20.674281463840938	31.353032016737657
25	31.32992131648344	17.659482963128482	23.663443922880663	27.34715179750741
26	29.557973421164196	19.092882057372695	20.60579114355112	30.743353377911987
27	29.71385585963387	21.752372673050935	20.591940028498097	27.9418314388171
28	30.049802793792864	22.913187945091426	19.945605676355306	27.091403584760403
29	30.004652750249853	23.224034516060406	19.447654263924196	27.323658469765544
30	31.163631410349613	21.86180413451958	18.417085388680654	28.557479066450153
31	28.85608155781425	21.44099042359924	20.590256467552422	29.11267155103409
32	28.917301955838663	24.366483668693572	18.57939596894289	28.13681840652487
33	30.543698354854854	23.17322158570014	19.164127295573614	27.118952763871395
34	29.490248355849687	23.947123942226312	19.476657427488263	27.085970274435738
35	29.1450418364895	25.003252333645047	20.032997794535163	25.81870803533029
36	31.02397237735641	24.392425812356418	19.82484844125214	24.758753369035027
37	30.243106200554966	24.44936078251913	19.23277066685849	26.07476235006742
38	29.77974431300765	26.1288658768215	20.16156063038644	23.929829179784413
39	30.964665116770256	23.571689851341567	20.221633145947898	25.242011885940276
40	30.621371734848335	25.756110178350323	19.508109406973308	24.11440867982803
41	30.040313632099082	26.04652444147866	19.301184461650777	24.61197746477149
42	31.48580375495311	24.825789704871767	18.36068609700066	25.327720443174464
43	30.089443001513672	26.003899739354154	17.531991484242635	26.374665774889532
44	30.267058681282016	25.352897331862	19.795845277688073	24.584198709167907
45	27.823676070629972	25.561276261637616	20.170743690090102	26.44430397764231
46	28.986021852621075	24.045535732050563	20.41677316465073	26.551669250677634
47	29.904633924977464	22.73924548920455	21.915218931795884	25.440901654022102
48	29.605725331623244	22.308177361615115	21.752372673050935	26.333724633710702
49	28.75369044211841	21.600163458462728	22.086865622756847	27.559280476662018
50	28.487687812702315	23.74739239367165	21.141622677259836	26.623297116366203
51	28.124803903412577	24.25896534466319	21.913382319855153	25.702848432069082
52	29.21108334085834	24.40887879432548	20.039808563815377	26.3402293010008
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	10.5
8	20.0
9	21.0
10	22.0
11	88.5
12	155.0
13	206.5
14	368.5
15	479.0
16	598.5
17	718.0
18	979.0
19	1240.0
20	1937.0
21	2634.0
22	4317.0
23	6000.0
24	8328.5
25	10657.0
26	14000.0
27	17343.0
28	16833.0
29	16323.0
30	14995.0
31	13667.0
32	12700.5
33	11734.0
34	13024.0
35	14314.0
36	13666.5
37	13019.0
38	13340.0
39	17414.0
40	21167.0
41	24131.0
42	27095.0
43	28672.0
44	30249.0
45	36283.0
46	42317.0
47	43793.5
48	45270.0
49	55743.0
50	66216.0
51	72014.5
52	77813.0
53	87976.0
54	98139.0
55	104643.5
56	111148.0
57	113002.5
58	114857.0
59	117259.0
60	119661.0
61	110621.5
62	101582.0
63	95353.5
64	83518.5
65	77912.0
66	66947.5
67	55983.0
68	49258.0
69	42533.0
70	36540.5
71	30548.0
72	23975.5
73	17403.0
74	13843.0
75	10283.0
76	6744.5
77	3206.0
78	2005.5
79	805.0
80	798.0
81	791.0
82	433.0
83	75.0
84	194.0
85	313.0
86	165.5
87	18.0
88	9.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1306754.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.273427699530288
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.87080425314403	9.434682582057087
2	8.589959975418925	2.1085650540032628
3	3.7139849667948006	1.367499779012951
4	2.1276427511036298	1.0445387790440046
5	1.3681134450681578	0.8395720726399668
6	0.929899498721907	0.6847832559235669
7	0.6167227850073483	0.5298511758708456
8	0.46121738325585776	0.45285745657258575
9	0.33667369362309235	0.3718926213315152
>10	2.391810750200625	5.601158994984318
>50	0.46247219284887503	4.200014044779523
>100	1.9196114118111673	55.4490096757103
>500	0.1938161472105244	15.22746502933719
>1k	0.017270745791036827	2.6881094787328657
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAATATCTCTGGGGGAGGCCATGGCCATGGGCAGCGTGGAGGCCTCTTATAA	2283	0.17470771086218218	No Hit
TTTTATCTCTGGGGTGGCAGTCCTCCTGCCTCAGCTTCCCAAGTGCTGAGTT	1657	0.1268027494080753	No Hit
GTCGATCTCTGGGTTGATACCACCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	1615	0.12358867851179334	No Hit
TGTTATCTCTGGGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATT	1551	0.11869104666983993	No Hit
TAGCATCTCTGGGCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCC	1549	0.11853799567477886	No Hit
TGTAATCTCTGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATC	1470	0.11249248136986761	No Hit
GGGCATCTCTGGGGGGAATTCGTGGAGAAAGAAATGGCCCGGTGGGCTGCAA	1377	0.10537561009952906	No Hit
GACGATCTCTGGGTGGCTAACACGGTGAAACCCCGTCTCTACCAAAAAATAC	1336	0.10223806470077766	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0026783924135682768	0.0	0.0	0.0	0.0
12	0.009948314678967885	0.0	0.0	0.0	0.0
13	0.012779758087597206	0.0	0.0	0.0	0.0
14	0.03765054478501692	0.0	0.0	0.0	0.0
15	0.06267438247749768	0.0	0.0	0.0	0.0
16	0.0796630429292736	0.0	0.0	0.0	0.0
17	0.0955038209180917	0.0	0.0	0.0	0.0
18	0.12833325935868573	0.0	0.0	0.0	0.0
19	0.1554998109820211	0.0	0.0	0.0	0.0
20	0.1813654291473376	0.0	0.0	0.0	0.0
21	0.21044511820893602	0.0	0.0	0.0	0.0
22	0.250008800432216	0.0	0.0	0.0	0.0
23	0.27947111698146704	0.0	0.0	0.0	0.0
24	0.3136780143776105	0.0	0.0	0.0	0.0
25	0.341303718986129	0.0	0.0	0.0	0.0
26	0.38293358964273305	0.0	0.0	0.0	0.0
27	0.4252521897771118	0.0	0.0	0.0	0.0
28	0.46320883655225087	0.0	0.0	0.0	0.0
29	0.5135626139273345	0.0	0.0	0.0	0.0
30	0.5871036170541662	0.0	0.0	0.0	0.0
31	0.6505432545069691	0.0	0.0	0.0	0.0
32	0.7028101693203158	0.0	0.0	0.0	0.0
33	0.7473480088830797	0.0	0.0	0.0	0.0
34	0.7957886488199003	0.0	0.0	0.0	0.0
35	0.837648095969096	0.0	0.0	0.0	0.0
36	0.8835633944874093	0.0	0.0	0.0	0.0
37	0.9247341121588302	0.0	0.0	0.0	0.0
38	0.9748583130413222	0.0	0.0	0.0	0.0
39	1.0268191258645467	0.0	0.0	0.0	0.0
40	1.0839071470223163	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATGCCC	115	0.0	46.000004	19
CCGTAAA	75	0.0	46.000004	19
CGTAAAC	75	0.0	46.000004	20
TCACGGT	60	0.0	46.0	27
CGGACAA	30	1.861601E-6	46.0	24
TCACGAA	55	1.8189894E-12	46.0	41
TATTTCG	20	6.3117745E-4	46.0	37
AACGTTT	30	1.861601E-6	46.0	29
TTTAGCG	25	3.4174136E-5	46.0	16
ATCCTAG	40	5.6115823E-9	46.0	36
CTATGTG	20	6.3117745E-4	46.0	44
AACGTGT	20	6.3117745E-4	46.0	14
ACTATTC	30	1.861601E-6	46.0	43
AGCAATT	45	3.110472E-10	46.0	44
AACGTAC	60	0.0	46.0	15
AACGTAA	30	1.861601E-6	46.0	16
GAAATAG	20	6.3117745E-4	46.0	14
CGGGTAC	30	1.861601E-6	46.0	46
TCGTTTA	60	0.0	46.0	22
ACTATAG	55	1.8189894E-12	46.0	14
>>END_MODULE