FastQCFastQC Report
Fri 17 Jun 2016
SRR1527934_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527934_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1334500
Sequences flagged as poor quality0
Sequence length59
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATATCTCTGGGGGAGGCCATGGCCATGGGCAGCGTGGAGGCCTCTTATAAGTGTTTA23630.1770700636942675No Hit
TTTTATCTCTGGGGTGGCAGTCCTCCTGCCTCAGCTTCCCAAGTGCTGAGTTTATAGGC15730.11787186212064443No Hit
TAGCATCTCTGGGCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAA15570.11667291120269765No Hit
TGTTATCTCTGGGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTG15450.11577369801423754No Hit
TGTAATCTCTGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGA14940.11195204196328212No Hit
GACGATCTCTGGGTGGCTAACACGGTGAAACCCCGTCTCTACCAAAAAATACAAAAAAT14210.10648182840014987No Hit
GGGCATCTCTGGGGGGAATTCGTGGAGAAAGAAATGGCCCGGTGGGCTGCAATAAAGGC13900.10415886099662795No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAA251.4788615E-553.00000441
ACTATTC251.4788615E-553.00000443
TACCCGT251.4788615E-553.00000435
CTACTAC251.4788615E-553.00000433
AGTCACG251.4788615E-553.00000439
ATAGGCG503.6379788E-1253.00000437
CGAAAGT550.053.00000438
CGTGATA251.4788615E-553.00000417
ACACCCG251.4788615E-553.00000415
GACGTAA251.4788615E-553.00000448
TTCACCG251.4788615E-553.00000429
CAATTCC750.053.00000425
CGACAAT251.4788615E-553.00000438
CGTTCAC251.4788615E-553.00000418
ATACTCA251.4788615E-553.00000417
CCACTAA251.4788615E-553.00000446
TAACCGG550.053.00000435
CGGATTA251.4788615E-553.00000424
TTAAGTC550.053.00000427
CTAAACT503.6379788E-1253.00000427