##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527934_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1334500
Sequences flagged as poor quality	0
Sequence length	59
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.51359310603222	31.0	31.0	34.0	30.0	34.0
2	31.99160134881978	33.0	31.0	34.0	30.0	34.0
3	32.21865118021731	34.0	31.0	34.0	30.0	34.0
4	35.82331360059948	37.0	35.0	37.0	35.0	37.0
5	35.64496515548895	37.0	35.0	37.0	33.0	37.0
6	35.8262907455976	37.0	35.0	37.0	35.0	37.0
7	35.67448932184339	37.0	35.0	37.0	33.0	37.0
8	35.79904608467591	37.0	35.0	37.0	33.0	37.0
9	37.45824803297115	39.0	37.0	39.0	34.0	39.0
10	37.7211090295991	39.0	37.0	39.0	35.0	39.0
11	37.94090895466467	39.0	37.0	39.0	35.0	39.0
12	37.85597377294867	39.0	37.0	39.0	35.0	39.0
13	37.86365155488947	39.0	38.0	39.0	35.0	39.0
14	38.11425852379168	40.0	38.0	40.0	34.0	40.0
15	37.598253278381414	40.0	37.0	40.0	33.0	40.0
16	37.18958186586737	39.0	36.0	40.0	32.0	40.0
17	37.0797377294867	39.0	36.0	40.0	32.0	40.0
18	37.13484301236418	39.0	36.0	40.0	32.0	40.0
19	37.2742053203447	40.0	36.0	40.0	32.0	40.0
20	37.42143199700262	40.0	37.0	40.0	32.0	40.0
21	37.394397901835895	40.0	36.0	40.0	32.0	40.0
22	37.385575121768454	40.0	36.0	40.0	32.0	40.0
23	37.29388759835144	40.0	36.0	40.0	32.0	40.0
24	37.26071337579618	40.0	36.0	40.0	32.0	40.0
25	37.207080554514796	40.0	36.0	40.0	32.0	40.0
26	37.02789509179468	40.0	36.0	40.0	31.0	40.0
27	36.916313975271635	39.0	36.0	40.0	31.0	40.0
28	36.88080029973773	39.0	36.0	40.0	31.0	40.0
29	36.78263019857624	39.0	35.0	40.0	31.0	40.0
30	36.749700262270515	39.0	35.0	40.0	31.0	40.0
31	36.715759460472086	39.0	35.0	40.0	31.0	40.0
32	36.60290745597602	39.0	35.0	40.0	30.0	40.0
33	36.47311802173098	39.0	35.0	40.0	30.0	40.0
34	36.412514799550394	39.0	35.0	40.0	30.0	40.0
35	36.261757212439115	39.0	35.0	40.0	30.0	40.0
36	36.07552341701012	38.0	35.0	40.0	30.0	40.0
37	35.938947920569504	38.0	35.0	40.0	30.0	40.0
38	35.717380292244286	38.0	35.0	40.0	29.0	40.0
39	35.542835518920946	38.0	34.0	40.0	28.0	40.0
40	35.298717871862124	38.0	34.0	40.0	27.0	40.0
41	35.127077557137504	38.0	34.0	40.0	27.0	40.0
42	34.98590558261521	37.0	34.0	40.0	27.0	40.0
43	34.71907680779318	37.0	33.0	40.0	26.0	40.0
44	34.53429748969651	37.0	33.0	40.0	25.0	40.0
45	34.28031022855002	36.0	33.0	40.0	24.0	40.0
46	34.02663694267516	36.0	33.0	40.0	23.0	40.0
47	33.86098763581866	36.0	33.0	39.0	23.0	40.0
48	33.58288497564631	35.0	33.0	39.0	23.0	40.0
49	33.40518621206444	35.0	32.0	39.0	23.0	40.0
50	33.16463094792057	35.0	32.0	39.0	22.0	40.0
51	32.919985013113525	35.0	32.0	38.0	21.0	40.0
52	32.67142675159236	35.0	32.0	38.0	20.0	40.0
53	32.325733233420756	35.0	31.0	38.0	20.0	40.0
54	32.070109404271264	35.0	31.0	37.0	19.0	40.0
55	31.83519595354065	35.0	31.0	37.0	18.0	40.0
56	31.568065192956162	35.0	30.0	37.0	18.0	39.0
57	31.323887598351444	35.0	30.0	37.0	15.0	39.0
58	31.0712544023979	34.0	30.0	36.0	15.0	39.0
59	30.48784263769202	34.0	29.0	36.0	13.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	5.0
14	6.0
15	26.0
16	93.0
17	219.0
18	598.0
19	1192.0
20	2119.0
21	3316.0
22	4942.0
23	7270.0
24	10126.0
25	13319.0
26	17315.0
27	22005.0
28	27236.0
29	32584.0
30	38728.0
31	46556.0
32	56559.0
33	70234.0
34	94617.0
35	124092.0
36	151779.0
37	234679.0
38	312896.0
39	61988.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.334657174971902	21.415736230798053	33.21626077182465	17.033345822405398
2	19.822480329711503	24.80854252529037	33.988160359685274	21.380816785312852
3	22.379692768827276	22.343799175721244	32.52251779692769	22.753990258523793
4	21.817609591607344	18.59685275384039	34.16418134132634	25.421356313225928
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	0.0	0.0	0.0	100.0
8	0.0	0.0	100.0	0.0
9	0.0	0.0	0.0	100.0
10	0.0	0.0	100.0	0.0
11	99.37324840764332	0.31457474709629074	0.2627201198950918	0.049456725365305355
12	99.43154739602848	0.20636942675159234	0.23731734732109405	0.12476582989883851
13	98.39925065567628	0.8783064818284001	0.233870363431997	0.4885724990633196
14	63.49014612214312	17.567028849756465	10.3047583364556	8.638066691644811
15	31.922592731360062	25.98059198201574	18.999775196702885	23.097040089921318
16	30.491719745222927	16.116298239040837	30.150468340202323	23.24151367553391
17	21.710303484451103	18.58613713001124	31.2129636568003	28.490595728737354
18	22.77137504683402	16.87081303859123	35.91255151742225	24.44526039715249
19	22.07740726863994	19.41790932933683	30.619632821281378	27.88505058074185
20	23.816410640689398	17.737279880104907	27.827500936680405	30.61880854252529
21	28.112701386286997	17.738703634319968	22.344323716747848	31.804271262645184
22	27.677482203072312	21.67703259647808	22.48092918696141	28.164556013488195
23	27.644511052828776	18.444960659423003	22.095091794679654	31.815436493068567
24	26.442712626451854	21.535256650430874	20.73375796178344	31.288272761333836
25	31.192506556762833	17.743724241288874	23.79857624578494	27.265192956163357
26	29.480779318096666	19.21738478831023	20.713076058448856	30.58875983514425
27	29.553091045335332	21.849531659797677	20.740427126264517	27.856950168602474
28	29.826002248032975	23.035593855376547	20.08265267890596	27.055751217684527
29	29.844511052828775	23.453503184713377	19.54267515923567	27.15931060322218
30	31.056500562008242	22.088347695766206	18.505732484076436	28.349419258149123
31	28.638666167103782	21.720194829524168	20.640989134507308	29.000149868864746
32	28.704233795429	24.732259273136005	18.640164855751216	27.923342075683777
33	30.41671037841888	23.46257025103035	19.21251405020607	26.908205320344695
34	29.342150618209068	24.214687148744847	19.589059572873737	26.854102660172348
35	28.956463094792056	25.29981266391907	20.07733233420757	25.666391907081305
36	30.76238291494942	24.830573248407646	19.794454852004495	24.612588984638442
37	30.003821656050956	24.749044585987264	19.238666167103784	26.008467590857997
38	29.62562757587111	26.44263769201948	20.205695016860247	23.726039715249154
39	30.739752716373175	23.794829524166357	20.298988385162982	25.16642937429749
40	30.315623829149494	26.146496815286625	19.51262645185463	24.025252903709255
41	29.876732858748596	26.335406519295617	19.28100412139378	24.50685650056201
42	31.325065567628325	25.185762457849382	18.30738104158861	25.181790932933684
43	30.027875608842265	26.313001124016484	17.381491195204195	26.277632071937056
44	29.6817534657175	25.89067066316973	19.9251405020607	24.50243536905208
45	27.632446609216938	25.87126264518546	20.097040089921318	26.399250655676283
46	28.887073810415885	24.219258149119522	20.418284001498687	26.475384038965906
47	29.64061446234545	23.11682278006744	21.882502810041213	25.3600599475459
48	29.4404645934807	22.554065192956163	21.624353690520795	26.381116523042337
49	28.43312101910828	22.037092544023977	21.953765455226677	27.576020981641065
50	28.229748969651553	24.10513300861746	20.999325590108654	26.665792431622332
51	27.95339078306482	24.60854252529037	21.8129636568003	25.62510303484451
52	28.894417384788312	24.86309479205695	19.97107530910453	26.27141251405021
53	26.909179467965533	25.572798801049085	22.059348070438368	25.45867366054702
54	27.646009741476206	24.43611839640315	22.191532409142	25.726339452978642
55	27.937954289996252	25.34507306107156	20.398726114649683	26.3182465342825
56	27.836118396403148	24.52439115773698	21.361109029599103	26.27838141626077
57	29.803896590483326	23.76155863619333	20.22862495316598	26.205919820157362
58	30.38126639190708	23.066017234919446	20.724241288872236	25.82847508430124
59	27.556762832521542	24.336230798051705	20.62817534657175	27.478831022854997
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	4.5
7	9.0
8	14.5
9	54.5
10	89.0
11	129.0
12	169.0
13	224.5
14	304.0
15	328.0
16	400.0
17	472.0
18	546.5
19	1080.0
20	1539.0
21	2242.0
22	2945.0
23	4529.5
24	6114.0
25	7449.0
26	10313.0
27	11842.0
28	12784.0
29	13726.0
30	14011.0
31	15440.0
32	16584.0
33	16274.5
34	15965.0
35	16030.5
36	16137.0
37	16178.0
38	15758.5
39	15339.0
40	18173.0
41	21007.0
42	25684.0
43	31062.5
44	31764.0
45	31020.0
46	30276.0
47	33722.5
48	42252.0
49	47335.0
50	53451.0
51	59567.0
52	70360.0
53	84954.0
54	88755.0
55	92138.0
56	95521.0
57	103964.0
58	108821.0
59	105235.0
60	100645.5
61	96056.0
62	87183.5
63	78311.0
64	81192.0
65	66474.0
66	48875.0
67	48036.5
68	47198.0
69	41842.0
70	31953.5
71	27421.0
72	23373.5
73	19326.0
74	14154.5
75	5912.5
76	2842.0
77	2083.5
78	1325.0
79	1029.0
80	733.0
81	713.0
82	408.0
83	123.0
84	92.5
85	62.0
86	50.5
87	23.5
88	8.0
89	4.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	1334500.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.12494346222364
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.47723701429231	14.466679077146221
2	6.23160539949271	2.1343178029040044
3	2.4445784498949954	1.255898032302663
4	1.3743115719771695	0.9414003187835487
5	0.859546490727941	0.7359842528434363
6	0.5354585665017735	0.5501818606623712
7	0.39960208600136493	0.4790214191112002
8	0.27488858002695904	0.37659611130980897
9	0.1976730466783862	0.30466257735355723
>10	1.4521440086701796	4.851993927510147
>50	0.3102225365502661	3.970806837106717
>100	1.2895955232231293	51.958102142433745
>500	0.14128947209181614	15.412762932245737
>1k	0.011847253871052905	2.5615927082868497
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAATATCTCTGGGGGAGGCCATGGCCATGGGCAGCGTGGAGGCCTCTTATAAGTGTTTA	2363	0.1770700636942675	No Hit
TTTTATCTCTGGGGTGGCAGTCCTCCTGCCTCAGCTTCCCAAGTGCTGAGTTTATAGGC	1573	0.11787186212064443	No Hit
TAGCATCTCTGGGCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAA	1557	0.11667291120269765	No Hit
TGTTATCTCTGGGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTG	1545	0.11577369801423754	No Hit
TGTAATCTCTGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGA	1494	0.11195204196328212	No Hit
GACGATCTCTGGGTGGCTAACACGGTGAAACCCCGTCTCTACCAAAAAATACAAAAAAT	1421	0.10648182840014987	No Hit
GGGCATCTCTGGGGGGAATTCGTGGAGAAAGAAATGGCCCGGTGGGCTGCAATAAAGGC	1390	0.10415886099662795	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.002847508430123642	0.0	0.0	0.0	0.0
12	0.008092918696140877	0.0	0.0	0.0	0.0
13	0.010565754964406145	0.0	0.0	0.0	0.0
14	0.03724241288872237	0.0	0.0	0.0	0.0
15	0.06294492319220682	0.0	0.0	0.0	0.0
16	0.07860621955788685	0.0	0.0	0.0	0.0
17	0.09441738478831023	0.0	0.0	0.0	0.0
18	0.12296740352191832	0.0	0.0	0.0	0.0
19	0.15211689771449982	0.0	0.0	0.0	0.0
20	0.1756463094792057	0.0	0.0	0.0	0.0
21	0.2041213937804421	0.0	0.0	0.0	0.0
22	0.24091420007493444	0.0	0.0	0.0	0.0
23	0.26616710378418884	0.0	0.0	0.0	0.0
24	0.2995878606219558	0.0	0.0	0.0	0.0
25	0.3250655676283252	0.0	0.0	0.0	0.0
26	0.3635069314349944	0.0	0.0	0.0	0.0
27	0.4053203446983889	0.0	0.0	0.0	0.0
28	0.44735856125889845	0.0	0.0	0.0	0.0
29	0.48909704008992133	0.0	0.0	0.0	0.0
30	0.554140127388535	0.0	0.0	0.0	0.0
31	0.6127388535031847	0.0	0.0	0.0	0.0
32	0.6658673660547021	0.0	0.0	0.0	0.0
33	0.7091045335331585	0.0	0.0	0.0	0.0
34	0.750917946796553	0.0	0.0	0.0	0.0
35	0.7919070813038591	0.0	0.0	0.0	0.0
36	0.8367928062944923	0.0	0.0	0.0	0.0
37	0.8802547770700637	0.0	0.0	0.0	0.0
38	0.9277632071937055	0.0	0.0	0.0	0.0
39	0.9796178343949045	0.0	0.0	0.0	0.0
40	1.0341701011614837	0.0	0.0	0.0	0.0
41	1.0902210565754964	0.0	0.0	0.0	0.0
42	1.1405020606968903	0.0	0.0	0.0	0.0
43	1.188010490820532	0.0	0.0	0.0	0.0
44	1.2346946421880853	0.0	0.0	0.0	0.0
45	1.2806294492319221	0.0	0.0	0.0	0.0
46	1.3192206819033345	0.0	0.0	0.0	0.0
47	1.3546646684151367	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAA	25	1.4788615E-5	53.000004	41
ACTATTC	25	1.4788615E-5	53.000004	43
TACCCGT	25	1.4788615E-5	53.000004	35
CTACTAC	25	1.4788615E-5	53.000004	33
AGTCACG	25	1.4788615E-5	53.000004	39
ATAGGCG	50	3.6379788E-12	53.000004	37
CGAAAGT	55	0.0	53.000004	38
CGTGATA	25	1.4788615E-5	53.000004	17
ACACCCG	25	1.4788615E-5	53.000004	15
GACGTAA	25	1.4788615E-5	53.000004	48
TTCACCG	25	1.4788615E-5	53.000004	29
CAATTCC	75	0.0	53.000004	25
CGACAAT	25	1.4788615E-5	53.000004	38
CGTTCAC	25	1.4788615E-5	53.000004	18
ATACTCA	25	1.4788615E-5	53.000004	17
CCACTAA	25	1.4788615E-5	53.000004	46
TAACCGG	55	0.0	53.000004	35
CGGATTA	25	1.4788615E-5	53.000004	24
TTAAGTC	55	0.0	53.000004	27
CTAAACT	50	3.6379788E-12	53.000004	27
>>END_MODULE