FastQCFastQC Report
Fri 17 Jun 2016
SRR1527933_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527933_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences400975
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATAAGGGGGGAGCTGACGTGGAAGCCTTGGGAGTGCCACCGGGAGCGGC14880.3710954548288547No Hit
TGCTTAAGGGGGGATCGCTGAGAGCCCCTCCCCATGGCTCACTGAGGAGCAC10880.271338612132926No Hit
GCCTTAAGGGGGGTCCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGA10510.2621111041835526No Hit
GTCGTAAGGGGGGACAGTCCAAAGTTGTGAAAAGTAGGGGCCGTGGAGCCGG10220.2548787330880977No Hit
CTGATAAGGGGGGACTTTGGCACATGCGGATAGTTGCCTTACAAGCACGAGG9410.2346779724421722No Hit
TTAGTAAGGGGGGAGTTCGAGACCAGCCTGGCCAACATGGCGAAACCCCGTC8800.21946505393104307No Hit
TTTATAAGGGGGGGGTAGACGGGTGTTTACTGTGCCATCTGTGTTTAAAATT8550.21323025126254755No Hit
CTTCTAAGGGGGGATCTAAAGAGTACACCCGTGCCAGGCGCGGTGGCTCACG8170.20375335120643429No Hit
GGTTTAAGGGTGGGGGCGGGGGTGGCTGACGCTGCCGGGTTGAGGGAGTTCC7640.19053556954922377No Hit
GGAATAAGGGGGGGAGTGCGAGATCCGCTGCTGCCGAGGAGAGGAGCGTCAA7460.18604651162790697No Hit
CGCTTAAGGGGGGGGTGGCTCAGTCCTGTAATCCCAGCACTTTGGGAGGCCA7060.1760708273583141No Hit
CCTTTAAGGGGGGGGTTCTCGCGAGAGCCGTCCCTCTTTCTAAGCCGGCGAT6930.17282872997069643No Hit
TTCTTAAGGGGGATCCCAGCACTTTGGGAGGCTGAGGCAAGTGGATCACCTG6730.16784088783589998No Hit
TAGTTAAGGGGGGGAGTTCGAGGCGCCGCCGCCGCCGCTGCAGCCGCCGGAG6650.16584575098198143No Hit
CAGTTAAGGGGGAGAGCTGACTCTGGGAGGCGTTTGGGCCCAGAGAAGTGGA6380.15911216410000623No Hit
CGGTTAAGGGGGGGAGTCGCGGGGTGCTTCCATAGTGTCTGGGAAGGGCCTT6350.15836398777978677No Hit
TCCATAAGGGGGGGCCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCG6350.15836398777978677No Hit
CACTTAAGGGGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGA6330.15786520356630712No Hit
TTGCTAAGGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCG6270.1563688509258682No Hit
TAAGTAAGGGGGGGGTGAAACCCTGTCTCTACTAACAATACTAAAATTAGCT6180.1541243219652098No Hit
AATTTAAGGGGGGGGGAGGGCACTCGCACTGGCGCCGAGTGCAAACAGACCA6010.14988465615063282No Hit
ACTTTAAGGGGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAG5960.1486376956169337No Hit
TATGTAAGGGGGGCTGTCCGAGCTGTATGGACTACAGGAGATATCAAGACAT5920.14764012718997443No Hit
TCACTAAGGGGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAA5730.14290167716191782No Hit
TACTTAAGGGGAGAGCGCGTCGTCAGTCCCGCTGGGAAGCTGTCAGTCATGG5700.14215350084169834No Hit
GGGTTAAGGGGGGCCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCAT5550.13841261924060105No Hit
GGCATAAGGGGGGGATTGCTGCTGCTTCTGAGCTTGCTCCCGCGCTTTCGGC5530.1379138350271214No Hit
GCACTAAGGGGGGGGAGCTCGCCCACCACCCGCAGAGACGCGACCCGCCATG5450.13591869817320282No Hit
GTGCTAAGGGGGGGCTCCTACGCTGAAGCGCTGCGAGAGGTAACTCTTCCCT5400.1346717376395037No Hit
AAATTAAGGGGGGGCCGCCATGGCGCCTGTGAAAAAGCTTGTGGCGAAGGGG5390.1344223455327639No Hit
GGTATAAGGGGGTAATGAGCTGACAGTCTGGTTGAGATCACCACCCCTTTTT5300.1321778165721055No Hit
CAAGTAAGGGGGGGCTTCCTCGGCAAGATGGCGGCCGCGGCCACCGGCCTGC5270.13142964025188603No Hit
TGCTTAAGGGGGGGTATTCCGGGCTCAGCCCTCCGACCCTGGGATGCTGCCC5240.13068146393166657No Hit
GAATTAAGGGGGGGAGTACACTAAGGGGGGCAATCGCTCTTGCCGTGCGAGA5230.13043207182492675No Hit
CTCATAAGGGGGGAACCGCTGTGACCATGTCCCGCTGCTAGCCACTCCTAAC5190.12943450339796744No Hit
TTGGTAAGGGGGGATGGGCGCCTGTAGTCCCAGCTTCTCGGGAGGCTGAGGC5170.12893571918448782No Hit
TTCGTAAGGGGGGCTCGAAACCAGGGTGCGCCTCTGGACCGCTAGGGGTATC5160.128686327077748No Hit
GGCATAAGGGGGGGGTATACCAGGAGGAGCCCGGAGGCTCCGGGGCCGTGAT5160.128686327077748No Hit
TTGGTAAGGGGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAG5030.1254442296901303No Hit
CATATAAGGGGGGAGGTGCGCTCCAAGATGGAGGAACAGACCCAGCAAATAC5020.1251948375833905No Hit
GAACTAAGGGGGGGCTTTCTGCCTCCTCTGCCGCCATGGCGCCTGTGAAAAA4980.1241972691564312No Hit
ATACTAAGGGGGGAAGGTGGCTAAGGGCCAGCCCCTCTGTGTGCTCAGCGCC4960.12369848494295156No Hit
GGGTTAAGGGGGGTGGATAAGGGGGGTCTTTTCTGCTGGGTGCCGGCTGCCT4880.12170334808903299No Hit
GCATTAAGGGGGGGCTCTTGTGGTCAGCCCAGTCCAGTCTCTGGTCTCTGTA4870.12145395598229317No Hit
TATATAAGGGGGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAG4830.12045638755533386No Hit
GGCATAAGGGGGGTGATGGCTCATGCCTGTAATCCTGGCACTTTGGGAGGCC4810.11995760334185424No Hit
ACTCTAAGGGGGGGACGTAATAACGGAGCTGGAGCCCATAGATGATGCCTGG4760.11871064280815512No Hit
GTAATAAGGGGGGGGTGACTTGATTGCTGCTGCTTCTGAGCTTGCTCCCGCG4750.11846125070141529No Hit
GCTCTAAGGGGGGCTTTTCCCTTTCTGCCACCGCCATGCCATCCAGACTGAG4710.11746368227445601No Hit
ATATTAAGGGGGGCACCTCCTGGGTACAGCCCAGGAGGACCTTGTGTGATCA4660.11621672174075691No Hit
TAAATAAGGGGGCTTCTCTTCCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC4620.11521915331379762No Hit
TAGGTAAGGGGGGATGGCCGCCGCCGTCGCTGCAGCCGGCGCTGGGGAGCCT4600.11472036910031796No Hit
TTTCTAAGGGGGCCCTTCCTGGGCGGCGGCGGAGCTGGTAACCAAGGTAGAA4560.11372280067335869No Hit
CGGGTAAGGGGGGAGATCTTTAAAATTTCCAGGCTAGGCCGGGCATGGTGGC4560.11372280067335869No Hit
GTAATAAGGGGGGAGATCAAGATCATTGCTCCTCCTGAGCGCAAGTACTCTG4540.11322401645987903No Hit
GACTTAAGGGGGGCTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCAAGT4500.11222644803291976No Hit
GGCATAAGGGGGGGATCTAATTACTATGGCTCCTTGAGCCAATAGTAATTAG4480.11172766381944012No Hit
GGCGTAAGGGGGGCGATACGACGACGAGTCGTCGGAGACACGCAGGGATGAG4430.11048070328574101No Hit
AGGTTAAGGGGGTGTGACTTGATTGCTGCTGCTTCTGAGCTTGCTCCCGCGC4390.10948313485878171No Hit
ATCGTAAGGGGTGGACTTGATTGCTGCTGCTTCTGAGCTTGCTCCCGCGCTT4370.10898435064530208No Hit
TATATAAGGGGGGACTTGGAAGTAGACCTGTTGTGATAGTGCCTTCTTGTTG4260.10624103747116403No Hit
TGCATAAGGGGGGAAATATGTTAATTACCAAAACCATTCTCTGAGAAAAAAA4160.10374711640376581No Hit
AATTTAAGGGGGGGTGCCCTGAGGGTGCCTGCTCCTTTGTACGGTAACAGTC4160.10374711640376581No Hit
TGACTAAGGGGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCATG4150.10349772429702601No Hit
TCTATAAGGGGGGTCCTTCCCCTCTGCTCGCCTCTGTCCTCATGAGCCTTGC4120.10274954797680654No Hit
ACTTTAAGGGGGGGGAGACTGCACCATGGCCCCGGACCCCTGGTTCTCCACA4110.10250015587006671No Hit
GAACTAAGGGGGTGGGCACGCGAGGGCGCCGAGCGCGGTGTGAGTGCCATCC4070.10150258744310743No Hit
CCTTTAAGGGGGGGGGACCTTGATCTGTAGCCCTCCCTGCTGCCATTCCCCT4060.1012531953363676No Hit
GGAGTAAGGGGGGGCCTTTTCCGGTCGCGGCGCTGCGCGGTGTGGAGCTCGT4020.10025562690940831No Hit
ATGGTAAGGGGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACA4010.10000623480266849No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCAG405.5952114E-946.00000435
CTTGTCT405.5952114E-946.00000443
ATCCTTG700.046.00000443
TATTTGT206.3065125E-446.00000443
GTGTTGC700.046.00000432
TCGCCAC206.3065125E-446.00000424
GTTGACC206.3065125E-446.00000439
ATTTAAC405.5952114E-946.00000420
CTATGTG351.0173426E-746.00000441
CAGGTAC351.0173426E-746.00000437
GACAGTA206.3065125E-446.00000419
CATGGAA206.3065125E-446.00000426
GGAATGT206.3065125E-446.00000414
AGCGTCA700.046.00000445
AGGTAAC700.046.00000438
CGGAAAT206.3065125E-446.00000443
GACAGCT206.3065125E-446.00000413
AACCGAG206.3065125E-446.00000421
CTGTCGA351.0173426E-746.00000442
CTGTCCG700.046.00000414
Produced by FastQC (version 0.11.5)