FastQCFastQC Report
Fri 17 Jun 2016
SRR1527932_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527932_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences398773
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATAAGGGGGGAGCTGACGTGGAAGCCTTGGGAGTGCCACCGGGAGCGGC13410.3362815436351007No Hit
TGCTTAAGGGGGGATCGCTGAGAGCCCCTCCCCATGGCTCACTGAGGAGCAC10650.2670692348779882No Hit
GCCTTAAGGGGGGTCCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGA9990.2505184653925918No Hit
GTCGTAAGGGGGGACAGTCCAAAGTTGTGAAAAGTAGGGGCCGTGGAGCCGG9340.23421846514182254No Hit
CTGATAAGGGGGGACTTTGGCACATGCGGATAGTTGCCTTACAAGCACGAGG9260.23221231126480477No Hit
CTTCTAAGGGGGGATCTAAAGAGTACACCCGTGCCAGGCGCGGTGGCTCACG8230.20638308009820125No Hit
TTAGTAAGGGGGGAGTTCGAGACCAGCCTGGCCAACATGGCGAAACCCCGTC7970.19986307999789354No Hit
TTTATAAGGGGGGGGTAGACGGGTGTTTACTGTGCCATCTGTGTTTAAAATT7650.19183846448982253No Hit
TAGTTAAGGGGGGGAGTTCGAGGCGCCGCCGCCGCCGCTGCAGCCGCCGGAG6870.17227846418889944No Hit
CGCTTAAGGGGGGGGTGGCTCAGTCCTGTAATCCCAGCACTTTGGGAGGCCA6840.1715261564850178No Hit
GGAATAAGGGGGGGAGTGCGAGATCCGCTGCTGCCGAGGAGAGGAGCGTCAA6780.17002154107725448No Hit
GGTTTAAGGGTGGGGGCGGGGGTGGCTGACGCTGCCGGGTTGAGGGAGTTCC6680.1675138487309823No Hit
CGGTTAAGGGGGGGAGTCGCGGGGTGCTTCCATAGTGTCTGGGAAGGGCCTT6540.1640030794462012No Hit
TTCTTAAGGGGGATCCCAGCACTTTGGGAGGCTGAGGCAAGTGGATCACCTG6440.16149538709992903No Hit
CACTTAAGGGGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGA6400.16049231016142015No Hit
TAAGTAAGGGGGGGGTGAAACCCTGTCTCTACTAACAATACTAAAATTAGCT6380.15999077169216572No Hit
AATTTAAGGGGGGGGGAGGGCACTCGCACTGGCGCCGAGTGCAAACAGACCA6360.15948923322291128No Hit
CCTTTAAGGGGGGGGTTCTCGCGAGAGCCGTCCCTCTTTCTAAGCCGGCGAT6320.1584861562844024No Hit
TCCATAAGGGGGGGCCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCG6120.153470771591858No Hit
TTGCTAAGGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCG5920.14845538689931365No Hit
CAGTTAAGGGGGAGAGCTGACTCTGGGAGGCGTTTGGGCCCAGAGAAGTGGA5890.147703079195432No Hit
ACTTTAAGGGGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAG5840.1464492330222959No Hit
TATGTAAGGGGGGCTGTCCGAGCTGTATGGACTACAGGAGATATCAAGACAT5520.13842461751422488No Hit
AAATTAAGGGGGGGCCGCCATGGCGCCTGTGAAAAAGCTTGTGGCGAAGGGG5510.13817384827959767No Hit
GTGCTAAGGGGGGGCTCCTACGCTGAAGCGCTGCGAGAGGTAACTCTTCCCT5450.13666923287183436No Hit
TCACTAAGGGGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAA5370.1346630789948166No Hit
TGCTTAAGGGGGGGTATTCCGGGCTCAGCCCTCCGACCCTGGGATGCTGCCC5300.13290769435242605No Hit
GGCATAAGGGGGGGATTGCTGCTGCTTCTGAGCTTGCTCCCGCGCTTTCGGC5240.13140307894466274No Hit
CAAGTAAGGGGGGGCTTCCTCGGCAAGATGGCGGCCGCGGCCACCGGCCTGC5190.13014923277152665No Hit
CTCATAAGGGGGGAACCGCTGTGACCATGTCCCGCTGCTAGCCACTCCTAAC5150.1291461558330178No Hit
ATACTAAGGGGGGAAGGTGGCTAAGGGCCAGCCCCTCTGTGTGCTCAGCGCC5130.12864461736376334No Hit
TATATAAGGGGGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAG5110.1281430788945089No Hit
TACTTAAGGGGAGAGCGCGTCGTCAGTCCCGCTGGGAAGCTGTCAGTCATGG5060.1268892327213728No Hit
GCACTAAGGGGGGGGAGCTCGCCCACCACCCGCAGAGACGCGACCCGCCATG5050.1266384634867456No Hit
GGTATAAGGGGGTAATGAGCTGACAGTCTGGTTGAGATCACCACCCCTTTTT5000.1253846173136095No Hit
TTGGTAAGGGGGGATGGGCGCCTGTAGTCCCAGCTTCTCGGGAGGCTGAGGC4960.12438154037510063No Hit
GGGTTAAGGGGGGCCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCAT4950.1241307711404734No Hit
GGCATAAGGGGGGGGTATACCAGGAGGAGCCCGGAGGCTCCGGGGCCGTGAT4890.12262615573271009No Hit
GAATTAAGGGGGGGAGTACACTAAGGGGGGCAATCGCTCTTGCCGTGCGAGA4850.12162307879420121No Hit
TTGGTAAGGGGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAG4790.1201184633864379No Hit
TTCGTAAGGGGGGCTCGAAACCAGGGTGCGCCTCTGGACCGCTAGGGGTATC4770.11961692491718345No Hit
TAAATAAGGGGGCTTCTCTTCCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC4770.11961692491718345No Hit
GAACTAAGGGGGGGCTTTCTGCCTCCTCTGCCGCCATGGCGCCTGTGAAAAA4750.11911538644792902No Hit
GCTCTAAGGGGGGCTTTTCCCTTTCTGCCACCGCCATGCCATCCAGACTGAG4710.11811230950942014No Hit
GGCATAAGGGGGGTGATGGCTCATGCCTGTAATCCTGGCACTTTGGGAGGCC4690.1176107710401657No Hit
GAATTAAGGGGGGGTCTTTCTGCGCTCTCGCTGGACAAGTGAGCAGGGACGG4680.1173600018055385No Hit
TTTCTAAGGGGGCCCTTCCTGGGCGGCGGCGGAGCTGGTAACCAAGGTAGAA4650.11660769410165683No Hit
GTAATAAGGGGGGGGTGACTTGATTGCTGCTGCTTCTGAGCTTGCTCCCGCG4630.11610615563240238No Hit
ATCGTAAGGGGTGGACTTGATTGCTGCTGCTTCTGAGCTTGCTCCCGCGCTT4610.11560461716314796No Hit
ACTCTAAGGGGGGGACGTAATAACGGAGCTGGAGCCCATAGATGATGCCTGG4580.11485230945926629No Hit
GCATTAAGGGGGGGCTCTTGTGGTCAGCCCAGTCCAGTCTCTGGTCTCTGTA4570.11460154022463907No Hit
GACTTAAGGGGGGCTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCAAGT4470.11209384787836689No Hit
GGGTTAAGGGGGGTGGATAAGGGGGGTCTTTTCTGCTGGGTGCCGGCTGCCT4430.11109077093985802No Hit
CATATAAGGGGGGAGGTGCGCTCCAAGATGGAGGAACAGACCCAGCAAATAC4380.10983692476672192No Hit
ATGATAAGGGGGGGCCCTTTCGGTCTTCGCGCTCTGCCGAGCAAGGTCGTGT4340.10883384782821304No Hit
TATATAAGGGGGGACTTGGAAGTAGACCTGTTGTGATAGTGCCTTCTTGTTG4340.10883384782821304No Hit
GGCATAAGGGGGGGATCTAATTACTATGGCTCCTTGAGCCAATAGTAATTAG4300.10783077088970416No Hit
GGCGTAAGGGGGGCGATACGACGACGAGTCGTCGGAGACACGCAGGGATGAG4250.10657692471656807No Hit
ATATTAAGGGGGGCACCTCCTGGGTACAGCCCAGGAGGACCTTGTGTGATCA4150.10406923237029589No Hit
GAACTAAGGGGGTGGGCACGCGAGGGCGCCGAGCGCGGTGTGAGTGCCATCC4070.10206307849327813No Hit
CGGCTAAGGGGGGGTCCCTGAGATCTGGGCTCCCTTACATTCTAGACCTATG4020.10080923232014204No Hit
GGAGTAAGGGGGGGCCTTTTCCGGTCGCGGCGCTGCGCGGTGTGGAGCTCGT4020.10080923232014204No Hit
CGGGTAAGGGGGGAGATCTTTAAAATTTCCAGGCTAGGCCGGGCATGGTGGC3990.10005692461626037No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCT351.0173244E-746.00000431
TTGATGG351.0173244E-746.00000434
AGACTTA700.046.00000418
CAGGTAC351.0173244E-746.00000437
CGTGGAA1400.046.00000420
ATCTCTC351.0173244E-746.00000443
CTGTCGG351.0173244E-746.00000441
ACCGGCC700.046.00000443
GATGTTC351.0173244E-746.00000436
GTATTTG351.0173244E-746.00000417
ACGCCAT351.0173244E-746.00000441
GATAAGC351.0173244E-746.00000434
ATAGGAG351.0173244E-746.00000420
ACTTGAG351.0173244E-746.00000424
GTCCTGA351.0173244E-746.00000420
CTGAGTA351.0173244E-746.00000425
TAGGTAT351.0173244E-746.00000414
GTGGGCA351.0173244E-746.00000412
TGGCGTG351.0173244E-746.00000426
TTATGGA351.0173244E-746.00000417
Produced by FastQC (version 0.11.5)