FastQCFastQC Report
Fri 17 Jun 2016
SRR1527931_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527931_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406374
Sequences flagged as poor quality0
Sequence length59
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTAAGGGGGGATCGCTGAGAGCCCCTCCCCATGGCTCACTGAGGAGCACTCAGCCA10680.2628120893561104No Hit
CTGATAAGGGGGGACTTTGGCACATGCGGATAGTTGCCTTACAAGCACGAGGTATTTAC9780.2406650031744157No Hit
GTCGTAAGGGGGGACAGTCCAAAGTTGTGAAAAGTAGGGGCCGTGGAGCCGGGCATGGT9680.23820421582089404No Hit
GCCTTAAGGGGGGTCCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTCTGT9350.23008361755427267No Hit
TTAGTAAGGGGGGAGTTCGAGACCAGCCTGGCCAACATGGCGAAACCCCGTCTCTACTA8110.19956985437060443No Hit
CTTCTAAGGGGGGATCTAAAGAGTACACCCGTGCCAGGCGCGGTGGCTCACGCCTGTAA8050.19809338195849147No Hit
TTTATAAGGGGGGGGTAGACGGGTGTTTACTGTGCCATCTGTGTTTAAAATTTTTCCCG7760.1909570986332787No Hit
GGAATAAGGGGGGGAGTGCGAGATCCGCTGCTGCCGAGGAGAGGAGCGTCAACTGCGGC7280.17914531933637487No Hit
CAGTTAAGGGGGAGAGCTGACTCTGGGAGGCGTTTGGGCCCAGAGAAGTGGATCGGTAG6760.16634922509806238No Hit
CGCTTAAGGGGGGGGTGGCTCAGTCCTGTAATCCCAGCACTTTGGGAGGCCAAGGAGGG6750.1661031463627102No Hit
TAGTTAAGGGGGGGAGTTCGAGGCGCCGCCGCCGCCGCTGCAGCCGCCGGAGCCGAGAT6590.1621658865970756No Hit
CCTTTAAGGGGGGGGTTCTCGCGAGAGCCGTCCCTCTTTCTAAGCCGGCGATCCGTCAG6340.1560139182132715No Hit
ACTTTAAGGGGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAGCTGACTG6330.15576783947791936No Hit
TAAGTAAGGGGGGGGTGAAACCCTGTCTCTACTAACAATACTAAAATTAGCTGAGCGTG6250.15379920959510204No Hit
TTCTTAAGGGGGATCCCAGCACTTTGGGAGGCTGAGGCAAGTGGATCACCTGAGGTTGG6180.1520766584476369No Hit
TTGCTAAGGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC6160.15158450097693257No Hit
AATTTAAGGGGGGGGGAGGGCACTCGCACTGGCGCCGAGTGCAAACAGACCATGAAGAC6080.14961587109411528No Hit
CACTTAAGGGGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACA6030.14838547741735447No Hit
TCCATAAGGGGGGGCCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC5880.144694296387072No Hit
GCACTAAGGGGGGGGAGCTCGCCCACCACCCGCAGAGACGCGACCCGCCATGCACGTGA5770.14198743029819821No Hit
TATGTAAGGGGGGCTGTCCGAGCTGTATGGACTACAGGAGATATCAAGACATCAGTGGA5690.14001880041538092No Hit
TATATAAGGGGGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTCTGTC5570.13706585559115494No Hit
AAATTAAGGGGGGGCCGCCATGGCGCCTGTGAAAAAGCTTGTGGCGAAGGGGGGCAAAA5500.1353433044436898No Hit
CGGTTAAGGGGGGGAGTCGCGGGGTGCTTCCATAGTGTCTGGGAAGGGCCTTCCCTGCA5500.1353433044436898No Hit
GGCATAAGGGGGGGGTATACCAGGAGGAGCCCGGAGGCTCCGGGGCCGTGATGTCAGAG5490.13509722570833765No Hit
ATACTAAGGGGGGAAGGTGGCTAAGGGCCAGCCCCTCTGTGTGCTCAGCGCCATGAAGA5460.13435898950228115No Hit
TCACTAAGGGGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCAT5410.13312859582552033No Hit
GTGCTAAGGGGGGGCTCCTACGCTGAAGCGCTGCGAGAGGTAACTCTTCCCTTCTTTTT5390.132636438354816No Hit
CAAGTAAGGGGGGGCTTCCTCGGCAAGATGGCGGCCGCGGCCACCGGCCTGCGCCAAGC5390.132636438354816No Hit
TTGGTAAGGGGGGATGGGCGCCTGTAGTCCCAGCTTCTCGGGAGGCTGAGGCAGGAGAA5350.13165212341340737No Hit
GCCATAAGGGGGGAGCTGACGTGGAAGCCTTGGGAGTGCCACCGGGAGCGGCGGGGCCC5300.13042172973664654No Hit
GGCATAAGGGGGGGATTGCTGCTGCTTCTGAGCTTGCTCCCGCGCTTTCGGCATCCGTA5230.1286991785891814No Hit
GGTATAAGGGGGTAATGAGCTGACAGTCTGGTTGAGATCACCACCCCTTTTTTTCCACA5210.12820702111847707No Hit
GGCATAAGGGGGGTGATGGCTCATGCCTGTAATCCTGGCACTTTGGGAGGCCGAGGTGG5110.12574623376495545No Hit
GAACTAAGGGGGGGCTTTCTGCCTCCTCTGCCGCCATGGCGCCTGTGAAAAAGCTTGTG5110.12574623376495545No Hit
GGGTTAAGGGGGGCCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCATGAAGGCC4920.12107073779326434No Hit
TTGGTAAGGGGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAGCTGACTG4890.12033250158720785No Hit
GCATTAAGGGGGGGCTCTTGTGGTCAGCCCAGTCCAGTCTCTGGTCTCTGTATTTTCAC4880.12008642285185568No Hit
TACTTAAGGGGAGAGCGCGTCGTCAGTCCCGCTGGGAAGCTGTCAGTCATGGCGCTGTC4870.1198403441165035No Hit
GAATTAAGGGGGGGAGTACACTAAGGGGGGCAATCGCTCTTGCCGTGCGAGATGCTAGC4840.11910210791044702No Hit
CTCATAAGGGGGGAACCGCTGTGACCATGTCCCGCTGCTAGCCACTCCTAACCCTGCCC4740.11664132055692539No Hit
GCTCTAAGGGGGGCTTTTCCCTTTCTGCCACCGCCATGCCATCCAGACTGAGGAAGACC4720.11614916308622107No Hit
GAGTTAAGGGGGGCTGATGGAGAAGATACAGGCCTCTGTGGCTACCAACCCCATCATCA4690.11541092688016458No Hit
TTCGTAAGGGGGGCTCGAAACCAGGGTGCGCCTCTGGACCGCTAGGGGTATCTCAAGGC4660.1146726906741081No Hit
TGCTTAAGGGGGGGTATTCCGGGCTCAGCCCTCCGACCCTGGGATGCTGCCCACTATGA4660.1146726906741081No Hit
GAATTAAGGGGGGGTCTTTCTGCGCTCTCGCTGGACAAGTGAGCAGGGACGGCCTCAGG4570.11245798205593861No Hit
ATGGTAAGGGGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTA4520.1112275883791778No Hit
TATATAAGGGGGGACTTGGAAGTAGACCTGTTGTGATAGTGCCTTCTTGTTGTAGCAAG4480.11024327343776914No Hit
GGTTTAAGGGTGGGGGCGGGGGTGGCTGACGCTGCCGGGTTGAGGGAGTTCCTGCGGGT4460.10975111596706483No Hit
ACTCTAAGGGGGGGACGTAATAACGGAGCTGGAGCCCATAGATGATGCCTGGATGCGTG4430.10901287976100833No Hit
CATATAAGGGGGGAGGTGCGCTCCAAGATGGAGGAACAGACCCAGCAAATACGCCTGCA4320.10630601367213455No Hit
GTAATAAGGGGGGGGTGACTTGATTGCTGCTGCTTCTGAGCTTGCTCCCGCGCTTTCGG4240.10433738378931724No Hit
TCTATAAGGGGGGTCCTTCCCCTCTGCTCGCCTCTGTCCTCATGAGCCTTGCCACCTGG4220.1038452263186129No Hit
GAACTAAGGGGGTGGGCACGCGAGGGCGCCGAGCGCGGTGTGAGTGCCATCCGTGAGCG4210.10359914758326075No Hit
TAGGTAAGGGGGGATGGCCGCCGCCGTCGCTGCAGCCGGCGCTGGGGAGCCTCTGTCCC4200.10335306884790857No Hit
ATATTAAGGGGGGCACCTCCTGGGTACAGCCCAGGAGGACCTTGTGTGATCAGACCATT4200.10335306884790857No Hit
GGCATAAGGGGGGGATCTAATTACTATGGCTCCTTGAGCCAATAGTAATTAGATCCCCC4150.10212267517114776No Hit
AATTTAAGGGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAG4140.1018765964357956No Hit
GGGTTAAGGGGGGTGGATAAGGGGGGTCTTTTCTGCTGGGTGCCGGCTGCCTGCTGTCG4140.1018765964357956No Hit
GGACTAAGGGGGGTTACAGACTTACTTTTTAAGAAAAACTAATAAATTTCTTGTAATGG4140.1018765964357956No Hit
CGGGTAAGGGGGGAGATCTTTAAAATTTCCAGGCTAGGCCGGGCATGGTGGCTCACGCC4130.10163051770044344No Hit
ATCGTAAGGGGTGGACTTGATTGCTGCTGCTTCTGAGCTTGCTCCCGCGCTTTCGGCAT4120.10138443896509128No Hit
TTTCTAAGGGGGCCCTTCCTGGGCGGCGGCGGAGCTGGTAACCAAGGTAGAAACGCTCA4110.1011383602297391No Hit
TTGCTAAGGGGGGAGCCCAGAAACTTAGGATACCCAAGATATAAGATACAATTTGCTAA4110.1011383602297391No Hit
AATTTAAGGGGGGGTGCCCTGAGGGTGCCTGCTCCTTTGTACGGTAACAGTCAATTTTG4080.10040012402368262No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATGA550.053.00000453
AGGTAAC550.053.00000438
AGTCCAG750.053.00000431
GATCGCT1100.053.00000413
CGCGGTA550.053.00000437
ACGCAAC550.053.00000425
TGGAATT550.053.00000423
GCTATAA1950.053.0000041
CGACCCT550.053.00000434
TCTAGTG550.053.00000446
CGTTCGG550.053.00000432
TATTCCG550.053.00000415
GGGCATA750.053.00000411
ATAGTGC550.053.00000436
TAGTGCC550.053.00000437
TCGCGGT550.053.00000436
CGCCCAC550.053.00000420
GTACGAG550.053.00000414
TTCTCGG550.053.00000435
GCATAAA203.1358263E-453.036
Produced by FastQC (version 0.11.5)