FastQCFastQC Report
Fri 17 Jun 2016
SRR1527930_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527930_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142364
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGCAGGAGGGAGTTTGAGACCAGCCTGACCAACATGGGGAAACCCTGTC17461.2264336489561969No Hit
GTTGGCAGGAGGGGGAGATCGAGACCATCCTAGCTAACATGGTGAAACCCCA12150.8534460959231266No Hit
TTACGCAGGAGGGGGATGGCCACAAACCAGCAGTTGTTCAAGGAATCCCCGG10330.7256047877272344No Hit
CTTTGCAGGAGGGCTTCGACATCCTTACTGGCGCAAAAGATTATCTGGGCGA8350.5865246832064286No Hit
ACTTGCAGGAGGGACGCAGCCATGTTCACTTGCCTGTGGCTGTTTTCATGCT8140.5717737630299795No Hit
ACGAGCAGGAGGGTATTAAAAATACAAAAGTTAGCCGGGCATGGTGGCAGGC7510.5275210025006322No Hit
TATAGCAGGAGGGGTGGCGATAAGTCGTGCCTTATCCGGTAACTATCGTCTT6710.4713270208760642No Hit
GCTGGCAGGAGGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCAC6690.46992217133544995No Hit
ATTTGCAGGAGGGACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAG5160.36245118147846367No Hit
ACGCGCAGGAGGGTCCAGGACAGAGTAAAAGTAATGACAACAGAAAAGTTCA5090.357534208086314No Hit
TTTAGCAGGAGGGCTCTAACAAAAATACAAAAAATTACCCAGGCATGATGGC4890.343485712680172No Hit
GTTTGCAGGAGGGCGATGATAGCACATGGTAAAACAATTTTCCAATTTGCCC4620.3245202438818802No Hit
TGGCGCAGGAGGGGCATAGGAGAAAGTCTACATGACCTTGGGTTTGGCAAAG4410.30976932370543114No Hit
CAGTGCAGGAGGGTGGCCATAACTTTGCTTTTTACTGGACTTGTGTTGCCAT4180.29361355398836786No Hit
CACAGCAGGAGGGGTTGCTTGCAAAAATCGTGGCGATTTTATGTGCGCATCT3690.25919474024331995No Hit
CACTGCAGGAGGGTGCTTGAACCCAGGAGGCAGAGGTTGCAGTGAGCAGAGA3690.25919474024331995No Hit
CGGTGCAGGAGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAA3550.2493607934590205No Hit
GGGAGCAGGAGGATTCTATGCAGCTTAAAGCATTTATAATGGATAAAACTGC3380.23741957236379985No Hit
GGCAGCAGGAGACCATCCCACTGATGCTGAGATGAAGGGTGAGCGGAACAAT2910.20440560815936615No Hit
GCGAGCAGGAGGGGACTTGGAAGTTCTGTTACATCCTGTTAACCAGGCATGT2850.20019105953752353No Hit
TACTGCAGGAGGAAGCTGGAAGTATCATGCCACATGACTTCAAACTACACTA2830.19878620999690932No Hit
CTTAGCAGGAGGGATTACCATCTCTACGCCTTCCGGCAGTTCGATGGTACCA2820.19808378522660222No Hit
GTATGCAGGAGGAGGGCAATATTGTGAGACGTTGTCTCTATTAAAAATAAAT2720.19105953752353125No Hit
GCGAGCAGGAGGGAGATGTGATACACGTTTCTGGAAACTGCCTCGTCATGCG2720.19105953752353125No Hit
ATTTGCAGGAGGGGAGTGGATGCAGGAGGGATGTGGTCTACCCTTGACTGAA2690.18895226321260994No Hit
GACAGCAGGAGGGTTGCATCAGCCAAAGTTCAAACATAAACGTCACCTTAAA2640.18544013936107442No Hit
CGATGCAGGAGGGAAGAGAAGAACCTAAACAGTAGTGCATCTTATGGCCAAG2630.18473771459076732No Hit
TTGGGCAGGAGGGTCATTCTAGCAACTAATCAATTAATTGGGCCAATTATTT2600.18263044027984604No Hit
TATAGCAGGAGGGGGTAGGTCCGAATGGCACAAGCTTGTTCCGTTAGCTGGC2570.18052316596892473No Hit
CTGCGCAGGAGAGGCTTTGTATCCAGTGTGGATTATTTCTTTTGCAACATGG2540.17841589165800342No Hit
TGGCGCAGGAGGGGATACGACGACGAGTCGTCGGAGACACGCAGGGATGAGA2540.17841589165800342No Hit
GTACGCAGGAGGGTGCCAGAAATAAGGAAGTGCCCAAAAACAAAATGTGGGT2540.17841589165800342No Hit
CAAGGCAGGAGGGAAGCAGTGGTATCGGTAGAAGCAGGAAATAGCACCAAAG2440.17139164395493242No Hit
TCTAGCAGGAGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAG2400.168581944873704No Hit
AAATGCAGGAGGGGAGTTGTCGTGGTAGGGGCAGGGTGACGGTCCATCCCGC2390.16787952010339693No Hit
TGGCGCAGGAGGGAGGCGATACGACGACGAGTCGTCGGAGACACGCAGGGAT2390.16787952010339693No Hit
ATTGGCAGGAGGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGA2330.16366497148155434No Hit
AACCGCAGGAGGGGCGCGAGCCCTGGACTCGAAGCAGCCATGATGGAAGGCC2250.15804557331909752No Hit
ACACGCAGGACGCTTTTTCACGTTCTGGTTGGTTGTGGCCTGTTGATGCTAA2220.1559382990081762No Hit
CGTTGCAGGAGGGACAGCTTTCCACTGTCATTCGCCATAGAAGCCGATACCG2200.15453344946756203No Hit
CTTTGCAGGAGGGCAGCACGCCTGGCCTATTCCAAGTCTTATAATACACAGT2140.15031890084571942No Hit
GTGCGCAGGAGGGTTGTTCTGCAAGCTGCTGGTATTTTTTTCATTATCGAAT2100.147509201764491No Hit
GTGCGCAGGAGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGC2080.14610435222387683No Hit
GTGTGCAGGAGGGCCAAATCAGTAAATTTGTGCTACCATGTATTGCGGGCTC2080.14610435222387683No Hit
GTAAGCAGGAGGGGCGGCAGGAGGGGGGAATTCTCTTGCTTCAACAATAACG2020.1418898036020342No Hit
TAGGGCAGGAGGGGGGAATTCAGATGTATATATGATGTCCTTGGACGGGGTG1990.13978252929111293No Hit
TGTTGCAGGAGGGGGGAATTCCTGGAGATTGTCTCGTACGGTTAAGAGCCTC1960.13767525498019162No Hit
ACTAGCAGGAGGGAAGCAGTGGTATCATCGCAGAGTACGCGCAGGAGGGTCC1920.1348655558989632No Hit
GCGGGCAGGAGGGGTTTTGTTGAAACCCAGAAAGGATAAAATCCAGGCGACT1900.13346070635834903No Hit
AGTGGCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT1900.13346070635834903No Hit
GACGGCAGGAGGGGCACATTAAATTAATTCCTCTCAAAAAGGCCCAGCTCTT1890.1327582815880419No Hit
GCCGGCAGGATCGGGGCCCAGGGCGTCTTGGTGGTGGTGAGTGAGGTTTAGG1810.12713888342558513No Hit
GAAAGCAGGAGGGGGGAAAGGGAAATGATCAGACATTTGGGGACTACTGGAC1800.126436458655278No Hit
TCGTGCAGGAGGGGAAACTGAAAAATACCACATGTTCTCACTTAGAAATGAG1780.12503160911466382No Hit
GAGCGCAGGAGGGTGTGAACTAAACAACCTTCTATTGTAAGAAGATGCCATC1720.12081706049282123No Hit
AGCAGCAGGAGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGC1700.11941221095220701No Hit
ATAAGCAGGAGAAACATACGAAGGCGCATAACGATACCACTGACCCTCAGCA1690.11870978618189992No Hit
CCCTGCAGGAGGGGAAGAGAAAGATGGTGGAGCTTTAAAAGTACTTCGAGAT1680.11800736141159282No Hit
GCTGGCAGGAGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTT1660.11660251187097861No Hit
CTGGGCAGGAGGGTTCTCACTCATAGGTGGGAATTGAACAACAAGATCACTT1630.1144952375600573No Hit
TGTGGCAGGAGGGGCAGAGAAAGTGGTTGTGATCACGAAGCAAAGAAAACAA1630.1144952375600573No Hit
CGTTGCAGGAGGGTCAGGTTATGCTGTAACATCTTTGACTGAAGAAATAAAA1610.11309038801944311No Hit
CTTGGCAGGAGGGGGTCTCACCCTCAGAATATCCCCTACTTGAGGCTTTGCA1610.11309038801944311No Hit
AGGAGCAGGAAAGCGAGGGTATCCTACAAAGTCCAGCGTACCATAAACGCAA1600.11238796324913602No Hit
AGGGGCAGGAGGGGATTATTTTCTCTCGAAACGTTATACTTCGTTTCTTAAT1590.11168553847882892No Hit
CTTGGCAGGAGGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTG1580.1109831137085218No Hit
TTAGGCAGGAGGGGGGAATTCCGCCGGATAAAGGCTTCTTCGGTACTATACA1580.1109831137085218No Hit
GGCTGCAGGAGGGGGGAATTCCTGGAGATTGTCTCGTACGGTTAAGAGCCTC1570.11028068893821472No Hit
TGTTGCAGGAGGGTTCCCCCCTCTCTCTCCCTTTTTAAATGTGCTCAGATTT1560.10957826416790761No Hit
TTGAGCAGGAGGGACTTACAGGGAGCTCATTTCAATAAAGTTTCCAAGAACA1560.10957826416790761No Hit
TTCGGCAGGAGGGCAGGAGGGGAAAGTTTCTGTCCCCTCAAAATGTATATGC1550.10887583939760052No Hit
GATCGCAGGAGGGTGCGACACATTTAAAGCAGTGTGTAGAGGGAAATTTATA1540.10817341462729341No Hit
TAACGCAGGAGGGGCGAGATTCTGTCTCAAACAACAACAACAATAACAACAA1520.10676856508667922No Hit
GAAAGCAGGAGGGAAGCAGTGGTATCAACTCAGAGTGGCGGCAGGAGGGGAG1490.1046612907757579No Hit
GCGCGCAGGAGGGGATACGACGACGAGTCGTCGGAGACACGCAGGGATGAGA1490.1046612907757579No Hit
GCGCGCAGGAGGGTTATGATCAGTCATTAAGTGTATTAATTATTCATGTGTC1470.10325644123514371No Hit
ATAGGCAGGAGGGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCT1470.10325644123514371No Hit
TCCGGCAGGAGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGT1460.10255401646483663No Hit
GGAGGCAGGAGGATTGCTTGAACCCAGGAGGTGGATGTTGCAGTGAGCTGAG1450.10185159169452951No Hit
TTCTGCAGGAGGGGTGGTATCAACGCAGAGTTTTAGCAGGAGGGCTCTAACA1430.10044674215391532No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCCAC351.0111398E-746.00000415
TCCTCTA351.0111398E-746.00000425
ACAGTAG351.0111398E-746.00000429
TAGCGCA351.0111398E-746.0000041
TGTTGCC351.0111398E-746.00000444
GGAGACC351.0111398E-746.0000048
TTCACGT351.0111398E-746.00000417
ACGCCTT351.0111398E-746.00000426
GGTGAGC351.0111398E-746.00000438
TTCATCT351.0111398E-746.00000419
ATCTCTA351.0111398E-746.00000420
GTGTTGC351.0111398E-746.00000443
TCCCACT351.0111398E-746.00000416
TAAATGT351.0111398E-746.00000436
ATCTCCT351.0111398E-746.00000422
GCCCAGG351.0111398E-746.00000416
ATCTTAT351.0111398E-746.00000439
CTCCCTT351.0111398E-746.00000427
ACTTGTG351.0111398E-746.00000439
CGGGGTG351.0111398E-746.00000446
Produced by FastQC (version 0.11.5)