FastQCFastQC Report
Fri 17 Jun 2016
SRR1527929_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527929_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142181
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGCAGGAGGGAGTTTGAGACCAGCCTGACCAACATGGGGAAACCCTGTC16561.164712584663211No Hit
GTTGGCAGGAGGGGGAGATCGAGACCATCCTAGCTAACATGGTGAAACCCCA12090.8503245862668007No Hit
TTACGCAGGAGGGGGATGGCCACAAACCAGCAGTTGTTCAAGGAATCCCCGG9240.649875862457009No Hit
ACTTGCAGGAGGGACGCAGCCATGTTCACTTGCCTGTGGCTGTTTTCATGCT8460.5950162117301186No Hit
CTTTGCAGGAGGGCTTCGACATCCTTACTGGCGCAAAAGATTATCTGGGCGA7990.5619597555228898No Hit
ACGAGCAGGAGGGTATTAAAAATACAAAAGTTAGCCGGGCATGGTGGCAGGC7860.5528164804017415No Hit
GCTGGCAGGAGGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCAC7140.5021768028076888No Hit
TATAGCAGGAGGGGTGGCGATAAGTCGTGCCTTATCCGGTAACTATCGTCTT6250.43958053467059593No Hit
ATTTGCAGGAGGGACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAG5140.3615110317130981No Hit
ACGCGCAGGAGGGTCCAGGACAGAGTAAAAGTAATGACAACAGAAAAGTTCA5070.3565877297247874No Hit
TTTAGCAGGAGGGCTCTAACAAAAATACAAAAAATTACCCAGGCATGATGGC4910.3453344680372202No Hit
CAGTGCAGGAGGGTGGCCATAACTTTGCTTTTTACTGGACTTGTGTTGCCAT4470.3143879983964102No Hit
GTTTGCAGGAGGGCGATGATAGCACATGGTAAAACAATTTTCCAATTTGCCC4390.30876136755262656No Hit
TGGCGCAGGAGGGGCATAGGAGAAAGTCTACATGACCTTGGGTTTGGCAAAG4020.28273819990012733No Hit
CACTGCAGGAGGGTGCTTGAACCCAGGAGGCAGAGGTTGCAGTGAGCAGAGA3550.2496817436928985No Hit
CGGTGCAGGAGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAA3470.24405511284911488No Hit
CACAGCAGGAGGGGTTGCTTGCAAAAATCGTGGCGATTTTATGTGCGCATCT3340.23491183772796648No Hit
GGGAGCAGGAGGATTCTATGCAGCTTAAAGCATTTATAATGGATAAAACTGC3130.22014193176303443No Hit
GGCAGCAGGAGACCATCCCACTGATGCTGAGATGAAGGGTGAGCGGAACAAT2980.20959199893094013No Hit
GACAGCAGGAGGGTTGCATCAGCCAAAGTTCAAACATAAACGTCACCTTAAA2970.20888867007546721No Hit
GCGAGCAGGAGGGGACTTGGAAGTTCTGTTACATCCTGTTAACCAGGCATGT2830.19904206609884587No Hit
CTTAGCAGGAGGGATTACCATCTCTACGCCTTCCGGCAGTTCGATGGTACCA2680.18849213326675154No Hit
GCGAGCAGGAGGGAGATGTGATACACGTTTCTGGAAACTGCCTCGTCATGCG2680.18849213326675154No Hit
GTATGCAGGAGGAGGGCAATATTGTGAGACGTTGTCTCTATTAAAAATAAAT2570.18075551585654906No Hit
TCTAGCAGGAGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAG2560.18005218700107609No Hit
TGGCGCAGGAGGGAGGCGATACGACGACGAGTCGTCGGAGACACGCAGGGAT2460.17301889844634655No Hit
TGGCGCAGGAGGGGATACGACGACGAGTCGTCGGAGACACGCAGGGATGAGA2460.17301889844634655No Hit
TTGGGCAGGAGGGTCATTCTAGCAACTAATCAATTAATTGGGCCAATTATTT2410.1695022541689818No Hit
CGATGCAGGAGGGAAGAGAAGAACCTAAACAGTAGTGCATCTTATGGCCAAG2390.16809559645803587No Hit
TATAGCAGGAGGGGGTAGGTCCGAATGGCACAAGCTTGTTCCGTTAGCTGGC2370.16668893874708998No Hit
CTGCGCAGGAGAGGCTTTGTATCCAGTGTGGATTATTTCTTTTGCAACATGG2370.16668893874708998No Hit
ATTTGCAGGAGGGGAGTGGATGCAGGAGGGATGTGGTCTACCCTTGACTGAA2360.16598560989161704No Hit
ACACGCAGGACGCTTTTTCACGTTCTGGTTGGTTGTGGCCTGTTGATGCTAA2340.16457895218067112No Hit
TACTGCAGGAGGAAGCTGGAAGTATCATGCCACATGACTTCAAACTACACTA2290.16106230790330636No Hit
CTTTGCAGGAGGGCAGCACGCCTGGCCTATTCCAAGTCTTATAATACACAGT2280.1603589790478334No Hit
TAGGGCAGGAGGGGGGAATTCAGATGTATATATGATGTCCTTGGACGGGGTG2180.15332569049310385No Hit
GTGCGCAGGAGGGTTGTTCTGCAAGCTGCTGGTATTTTTTTCATTATCGAAT2150.151215703926685No Hit
ACTAGCAGGAGGGAAGCAGTGGTATCATCGCAGAGTACGCGCAGGAGGGTCC2110.14840238850479318No Hit
CAAGGCAGGAGGGAAGCAGTGGTATCGGTAGAAGCAGGAAATAGCACCAAAG2110.14840238850479318No Hit
ATTGGCAGGAGGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGA2100.14769905964932023No Hit
GAAAGCAGGAGGGGGGAAAGGGAAATGATCAGACATTTGGGGACTACTGGAC2100.14769905964932023No Hit
GCGGGCAGGAGGGGTTTTGTTGAAACCCAGAAAGGATAAAATCCAGGCGACT2070.14558907308290137No Hit
AAATGCAGGAGGGGAGTTGTCGTGGTAGGGGCAGGGTGACGGTCCATCCCGC2060.14488574422742842No Hit
AGCAGCAGGAGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGC2020.1420724288055366No Hit
GTGTGCAGGAGGGCCAAATCAGTAAATTTGTGCTACCATGTATTGCGGGCTC1990.13996244223911775No Hit
AACCGCAGGAGGGGCGCGAGCCCTGGACTCGAAGCAGCCATGATGGAAGGCC1930.13574246910628002No Hit
TGTGGCAGGAGGGGCAGAGAAAGTGGTTGTGATCACGAAGCAAAGAAAACAA1930.13574246910628002No Hit
GACGGCAGGAGGGGCACATTAAATTAATTCCTCTCAAAAAGGCCCAGCTCTT1920.13503914025080707No Hit
GTAAGCAGGAGGGGCGGCAGGAGGGGGGAATTCTCTTGCTTCAACAATAACG1900.13363248253986118No Hit
GTACGCAGGAGGGTGCCAGAAATAAGGAAGTGCCCAAAAACAAAATGTGGGT1860.13081916711796934No Hit
GCCGGCAGGATCGGGGCCCAGGGCGTCTTGGTGGTGGTGAGTGAGGTTTAGG1840.12941250940702342No Hit
CGTTGCAGGAGGGACAGCTTTCCACTGTCATTCGCCATAGAAGCCGATACCG1840.12941250940702342No Hit
GTGCGCAGGAGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGC1790.12589586512965867No Hit
TTAGGCAGGAGGGGGGAATTCCGCCGGATAAAGGCTTCTTCGGTACTATACA1780.12519253627418572No Hit
ATAGGCAGGAGGGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCT1760.12378587856323982No Hit
GCTGGCAGGAGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTT1750.12308254970776686No Hit
CTTGGCAGGAGGGGGTCTCACCCTCAGAATATCCCCTACTTGAGGCTTTGCA1750.12308254970776686No Hit
TCGTGCAGGAGGGGAAACTGAAAAATACCACATGTTCTCACTTAGAAATGAG1740.12237922085229391No Hit
TGTTGCAGGAGGGGGGAATTCCTGGAGATTGTCTCGTACGGTTAAGAGCCTC1730.12167589199682095No Hit
GGCTGCAGGAGGGGGGAATTCCTGGAGATTGTCTCGTACGGTTAAGAGCCTC1720.120972563141348No Hit
CCCTGCAGGAGGGGAAGAGAAAGATGGTGGAGCTTTAAAAGTACTTCGAGAT1670.11745591886398324No Hit
GAGCGCAGGAGGGTGTGAACTAAACAACCTTCTATTGTAAGAAGATGCCATC1660.11675259000851028No Hit
AGTGGCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT1650.11604926115303732No Hit
TGTTGCAGGAGGGTTCCCCCCTCTCTCTCCCTTTTTAAATGTGCTCAGATTT1640.11534593229756437No Hit
GCGTGCAGGAGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGC1600.11253261687567256No Hit
CTGGGCAGGAGGGTTCTCACTCATAGGTGGGAATTGAACAACAAGATCACTT1580.11112595916472665No Hit
TTCGGCAGGAGGGCAGGAGGGGAAAGTTTCTGTCCCCTCAAAATGTATATGC1580.11112595916472665No Hit
GCGCGCAGGAGGGTTATGATCAGTCATTAAGTGTATTAATTATTCATGTGTC1570.1104226303092537No Hit
CTTGGCAGGAGGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTG1560.10971930145378074No Hit
GAAAGCAGGAGGGAAGCAGTGGTATCAACTCAGAGTGGCGGCAGGAGGGGAG1540.10831264374283484No Hit
GTCGGCAGGAGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG1520.10690598603188893No Hit
ATAAGCAGGAGAAACATACGAAGGCGCATAACGATACCACTGACCCTCAGCA1520.10690598603188893No Hit
TTGAGCAGGAGGGACTTACAGGGAGCTCATTTCAATAAAGTTTCCAAGAACA1500.10549932832094303No Hit
TAACGCAGGAGGGGCGAGATTCTGTCTCAAACAACAACAACAATAACAACAA1490.10479599946547007No Hit
GCGCGCAGGAGGGGATACGACGACGAGTCGTCGGAGACACGCAGGGATGAGA1480.10409267060999712No Hit
AGGGGCAGGAGGGGATTATTTTCTCTCGAAACGTTATACTTCGTTTCTTAAT1480.10409267060999712No Hit
GATCGCAGGAGGGTGCGACACATTTAAAGCAGTGTGTAGAGGGAAATTTATA1470.10338934175452416No Hit
GAAGGCAGGAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT1460.10268601289905122No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCCAA206.292714E-446.00000420
ATCCTGT206.292714E-446.00000433
CGACACA206.292714E-446.00000416
GGATGTT206.292714E-446.00000433
AAGTATC206.292714E-446.00000420
GTAGAGG206.292714E-446.00000436
ACAAAGT206.292714E-446.00000426
ACGCCTT351.0111398E-746.00000426
TGCATGT206.292714E-446.00000428
GTTTGGC206.292714E-446.00000442
CCTATTC206.292714E-446.00000426
TCAAGTA206.292714E-446.00000415
GGTTGCT405.5515557E-946.00000413
ATCTCTA351.0111398E-746.00000420
AGCTCTA206.292714E-446.00000419
CATGACC206.292714E-446.00000431
TTGCAAC700.046.00000442
CACATTT206.292714E-446.00000419
GGGTTAG206.292714E-446.00000411
CAGCATT206.292714E-446.00000446
Produced by FastQC (version 0.11.5)