FastQCFastQC Report
Fri 17 Jun 2016
SRR1527928_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527928_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139134
Sequences flagged as poor quality0
Sequence length59
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGCAGGAGGGAGTTTGAGACCAGCCTGACCAACATGGGGAAACCCTGTCTCTACTA17181.2347808587404947No Hit
GTTGGCAGGAGGGGGAGATCGAGACCATCCTAGCTAACATGGTGAAACCCCATCTCTAC11540.8294162462086909No Hit
TTACGCAGGAGGGGGATGGCCACAAACCAGCAGTTGTTCAAGGAATCCCCGGTCCGTCT10060.7230439719982176No Hit
ACTTGCAGGAGGGACGCAGCCATGTTCACTTGCCTGTGGCTGTTTTCATGCTATGAGGT8150.5857662397401067No Hit
CTTTGCAGGAGGGCTTCGACATCCTTACTGGCGCAAAAGATTATCTGGGCGAGGCACTG8110.5828913134100938No Hit
ACGAGCAGGAGGGTATTAAAAATACAAAAGTTAGCCGGGCATGGTGGCAGGCACCTGTA7740.5562982448574756No Hit
TATAGCAGGAGGGGTGGCGATAAGTCGTGCCTTATCCGGTAACTATCGTCTTTAGTCCA6110.4391449969094542No Hit
ATTTGCAGGAGGGACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAA5460.3924274440467463No Hit
GTTTGCAGGAGGGCGATGATAGCACATGGTAAAACAATTTTCCAATTTGCCCAAGCTAC5420.3895525177167335No Hit
CAGTGCAGGAGGGTGGCCATAACTTTGCTTTTTACTGGACTTGTGTTGCCATTTTGAAC5170.37158422815415354No Hit
TTTAGCAGGAGGGCTCTAACAAAAATACAAAAAATTACCCAGGCATGATGGCGGCATGC5130.3687093018241408No Hit
ACGCGCAGGAGGGTCCAGGACAGAGTAAAAGTAATGACAACAGAAAAGTTCAGAGTGTT5050.36295944916411516No Hit
TGGCGCAGGAGGGGCATAGGAGAAAGTCTACATGACCTTGGGTTTGGCAAAGCTCTTTT4840.347866085931548No Hit
CACTGCAGGAGGGTGCTTGAACCCAGGAGGCAGAGGTTGCAGTGAGCAGAGATTGTACC3670.2637744907786738No Hit
CACAGCAGGAGGGGTTGCTTGCAAAAATCGTGGCGATTTTATGTGCGCATCTCCACATT3320.23861888539106185No Hit
GTATGCAGGAGGAGGGCAATATTGTGAGACGTTGTCTCTATTAAAAATAAATAGGCTGG3320.23861888539106185No Hit
GGGAGCAGGAGGATTCTATGCAGCTTAAAGCATTTATAATGGATAAAACTGCCAATACT3160.22711918007101067No Hit
GACAGCAGGAGGGTTGCATCAGCCAAAGTTCAAACATAAACGTCACCTTAAAATAAGAG3080.22136932741098508No Hit
CGGTGCAGGAGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTG3060.2199318642459787No Hit
GCGAGCAGGAGGGGACTTGGAAGTTCTGTTACATCCTGTTAACCAGGCATGTGGATACA3030.21777566949846913No Hit
GCTGGCAGGAGGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGT2930.21058835367343712No Hit
GCGAGCAGGAGGGAGATGTGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTC2800.20124484310089555No Hit
TTGGGCAGGAGGGTCATTCTAGCAACTAATCAATTAATTGGGCCAATTATTTAATTGAC2690.19333879569336035No Hit
TACTGCAGGAGGAAGCTGGAAGTATCATGCCACATGACTTCAAACTACACTACAGGGCT2610.18758894303333476No Hit
CTGCGCAGGAGAGGCTTTGTATCCAGTGTGGATTATTTCTTTTGCAACATGGTGCGGAA2510.18040162720830277No Hit
CTTAGCAGGAGGGATTACCATCTCTACGCCTTCCGGCAGTTCGATGGTACCAGTCACGT2480.1782454324607932No Hit
GTACGCAGGAGGGTGCCAGAAATAAGGAAGTGCCCAAAAACAAAATGTGGGTATGTCAA2470.17752670087828998No Hit
TCTAGCAGGAGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGG2380.1710581166357612No Hit
TATAGCAGGAGGGGGTAGGTCCGAATGGCACAAGCTTGTTCCGTTAGCTGGCATAAGAT2340.1681831903057484No Hit
CAAGGCAGGAGGGAAGCAGTGGTATCGGTAGAAGCAGGAAATAGCACCAAAGGGTGCTA2290.16458953239323243No Hit
CGATGCAGGAGGGAAGAGAAGAACCTAAACAGTAGTGCATCTTATGGCCAAGGCATAAG2260.16243333764572282No Hit
CTTTGCAGGAGGGCAGCACGCCTGGCCTATTCCAAGTCTTATAATACACAGTTCAACAG2260.16243333764572282No Hit
GTGCGCAGGAGGGTTGTTCTGCAAGCTGCTGGTATTTTTTTCATTATCGAATCGTAATT2230.16027714289821324No Hit
GCGGGCAGGAGGGGTTTTGTTGAAACCCAGAAAGGATAAAATCCAGGCGACTACCTTTT2220.15955841131571002No Hit
ACTAGCAGGAGGGAAGCAGTGGTATCATCGCAGAGTACGCGCAGGAGGGTCCAGGACAG2180.15668348498569723No Hit
TGGCGCAGGAGGGAGGCGATACGACGACGAGTCGTCGGAGACACGCAGGGATGAGATGG2130.15308982707318125No Hit
CGTTGCAGGAGGGACAGCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTGAAAGA2110.15165236390817485No Hit
GACGGCAGGAGGGGCACATTAAATTAATTCCTCTCAAAAAGGCCCAGCTCTTAGTCCCT2100.15093363232567164No Hit
GTAAGCAGGAGGGGCGGCAGGAGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTT2040.14662124283065248No Hit
ATTTGCAGGAGGGGAGTGGATGCAGGAGGGATGTGGTCTACCCTTGACTGAAACATCAT2010.14446504808314287No Hit
ACACGCAGGACGCTTTTTCACGTTCTGGTTGGTTGTGGCCTGTTGATGCTAAAGGTGAG2000.14374631650063968No Hit
GAAAGCAGGAGGGGGGAAAGGGAAATGATCAGACATTTGGGGACTACTGGACACTGGCT1970.14159012175313007No Hit
GTGCGCAGGAGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGCTCACAGT1930.13871519542311728No Hit
TAACGCAGGAGGGGCGAGATTCTGTCTCAAACAACAACAACAATAACAACAACAACAAC1900.1365590006756077No Hit
ATAAGCAGGAGAAACATACGAAGGCGCATAACGATACCACTGACCCTCAGCAATCTTAA1900.1365590006756077No Hit
TTGAGCAGGAGGGACTTACAGGGAGCTCATTTCAATAAAGTTTCCAAGAACATACATTG1880.1351215375106013No Hit
AAATGCAGGAGGGGAGTTGTCGTGGTAGGGGCAGGGTGACGGTCCATCCCGCTCTCCTG1850.1329653427630917No Hit
GCGCGCAGGAGGGTTATGATCAGTCATTAAGTGTATTAATTATTCATGTGTCTCAAAAC1830.13152787959808532No Hit
TCGTGCAGGAGGGGAAACTGAAAAATACCACATGTTCTCACTTAGAAATGAGAGCTAGA1800.1293716848505757No Hit
AGCAGCAGGAGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGCTCACAGT1760.1264967585205629No Hit
GCTGGCAGGAGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGAC1740.12505929535555652No Hit
CCCTGCAGGAGGGGAAGAGAAAGATGGTGGAGCTTTAAAAGTACTTCGAGATACAGTAA1740.12505929535555652No Hit
AACCGCAGGAGGGGCGCGAGCCCTGGACTCGAAGCAGCCATGATGGAAGGCCTGGATGA1720.12362183219055012No Hit
CTTGGCAGGAGGGGGTCTCACCCTCAGAATATCCCCTACTTGAGGCTTTGCAGTGTAAA1650.11859071111302773No Hit
GAAGGCAGGAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGA1640.11787197953052453No Hit
CTTGGCAGGAGGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGCTCACAG1640.11787197953052453No Hit
TGTTGCAGGAGGGTTCCCCCCTCTCTCTCCCTTTTTAAATGTGCTCAGATTTCTGGCCT1610.11571578478301495No Hit
TAAAGCAGGAATTACTACTGCTTGTTTACGAATTAAATCGAAGTGGACTGCTGGCGGAA1580.11355959003550534No Hit
AGGAGCAGGAAAGCGAGGGTATCCTACAAAGTCCAGCGTACCATAAACGCAAGCCTCAA1580.11355959003550534No Hit
TAGGGCAGGAGGGGGGAATTCAGATGTATATATGATGTCCTTGGACGGGGTGGCGCAGT1570.11284085845300214No Hit
GATCGCAGGAGGGTGCGACACATTTAAAGCAGTGTGTAGAGGGAAATTTATAGCACCAA1540.11068466370549254No Hit
GAGCGCAGGAGGGTGTGAACTAAACAACCTTCTATTGTAAGAAGATGCCATCTATAAGT1520.10924720054048616No Hit
CGTTGCAGGAGGGTCAGGTTATGCTGTAACATCTTTGACTGAAGAAATAAAAACTGCCT1480.10637227421047336No Hit
TTCGGCAGGAGGGCAGGAGGGGAAAGTTTCTGTCCCCTCAAAATGTATATGCTAAAATA1450.10421607946296375No Hit
TGTTGCAGGAGGGGGGAATTCCTGGAGATTGTCTCGTACGGTTAAGAGCCTCCGCCCGT1450.10421607946296375No Hit
TTGTGCAGGAGGTACACATGAAACTATAGCATAAATCACTATATTGTGACTTCTTATTT1400.10062242155044777No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGACG550.053.00000425
GGAGATT600.053.00000424
CAGCCAA150.006683957353.00000420
CTCGAAG150.006683957353.00000428
TTGTTTA150.006683957353.00000422
TGTGAGA550.053.00000423
TCCTCTA150.006683957353.00000425
CGCTGAA600.053.00000417
TGTGACT150.006683957353.00000445
GAGGTAG150.006683957353.00000440
ACAAAGT150.006683957353.00000426
TTGCATC150.006683957353.00000414
ATGGGAG150.006683957353.00000449
TTCACGT150.006683957353.00000417
AACGTCA150.006683957353.00000439
ACGCTTT150.006683957353.00000410
TGCATGT150.006683957353.00000428
TGCATGC150.006683957353.00000418
TCCTTAT150.006683957353.00000432
CAGTTTG150.006683957353.00000421
Produced by FastQC (version 0.11.5)