FastQCFastQC Report
Fri 17 Jun 2016
SRR1527927_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527927_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13170951
Sequences flagged as poor quality0
Sequence length52
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGTG13625400.044.1914525
ATTGTGG13545300.044.1744736
TTGTGGG13146250.044.1391837
TTATTGT5422400.044.1027034
ATATTGT2957550.044.055824
GTTATTG1115250.043.9686163
GTATTGT2839250.043.9683344
CGGTATT129100.043.9333842
TGTGGGG6999700.043.9026378
CGATATT106700.043.822872
ACGTATT98100.043.7961272
TCTATTG1066500.043.7593083
TGTGGGA3431250.043.7397168
TTTATTG2066900.043.738843
CTATTGT2554100.043.689294
TGTATTG1027950.043.657383
TATATTG1131900.043.6063273
AATATTG747650.043.5789453
CTTATTG1152250.043.5328253
GTGGGGG2557300.043.521299
Produced by FastQC (version 0.11.5)