FastQCFastQC Report
Fri 17 Jun 2016
SRR1527926_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527926_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13063257
Sequences flagged as poor quality0
Sequence length52
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGTG13515700.044.193455
ATTGTGG13444450.044.1707046
TTGTGGG13061550.044.1195457
TTATTGT5351400.044.075384
ATATTGT2955650.044.0740284
GTATTGT2813600.043.990694
TGTGGGG6964050.043.9067658
GTTATTG1108050.043.8972973
TTTATTG2032650.043.80713
TGTGGGA3427000.043.764438
ACGTATT95050.043.7496032
TCTATTG1063050.043.7239073
CTATTGT2541250.043.6812174
TATATTG1126500.043.662233
CGGTATT123450.043.633862
TGTATTG1014400.043.596613
CGTATTG579050.043.577073
AATATTG748800.043.5611653
CATATTG579650.043.492283
CTTATTG1133900.043.456393
Produced by FastQC (version 0.11.5)