##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527926_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13063257
Sequences flagged as poor quality	0
Sequence length	52
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.508209476396278	31.0	31.0	33.0	30.0	34.0
2	32.06949369517878	33.0	31.0	34.0	30.0	34.0
3	32.325400319384364	34.0	31.0	34.0	30.0	34.0
4	35.98762659266368	37.0	35.0	37.0	35.0	37.0
5	35.684907906198276	37.0	35.0	37.0	33.0	37.0
6	35.96628781015332	37.0	35.0	37.0	35.0	37.0
7	36.315449891248406	37.0	37.0	37.0	35.0	37.0
8	36.263700545736796	37.0	37.0	37.0	35.0	37.0
9	37.765382017669864	39.0	38.0	39.0	35.0	39.0
10	36.95724573129044	39.0	37.0	39.0	34.0	39.0
11	38.06327503164027	39.0	37.0	39.0	35.0	39.0
12	38.25135883034376	39.0	39.0	39.0	37.0	39.0
13	38.19338883097837	39.0	39.0	39.0	37.0	39.0
14	38.734963416856914	40.0	39.0	40.0	36.0	40.0
15	38.254896003347405	40.0	38.0	40.0	35.0	40.0
16	37.7769884646685	40.0	37.0	40.0	34.0	40.0
17	37.822837826738	40.0	37.0	40.0	33.0	40.0
18	37.86445248684918	40.0	38.0	40.0	33.0	40.0
19	37.982932204426504	40.0	38.0	40.0	34.0	40.0
20	37.98219999805561	40.0	38.0	40.0	34.0	40.0
21	37.946729670862325	40.0	38.0	40.0	34.0	40.0
22	37.918200415103215	40.0	38.0	40.0	34.0	40.0
23	37.85909616568058	40.0	37.0	40.0	34.0	40.0
24	37.832365159776	40.0	37.0	40.0	33.0	40.0
25	37.79768728426609	40.0	37.0	40.0	33.0	40.0
26	37.82588170775481	40.0	37.0	40.0	34.0	40.0
27	37.86740726298196	40.0	37.0	40.0	34.0	40.0
28	37.715759936438516	40.0	37.0	40.0	34.0	40.0
29	37.84117023801951	40.0	37.0	40.0	34.0	40.0
30	37.883801336833535	40.0	37.0	40.0	34.0	40.0
31	37.89576037583889	40.0	37.0	40.0	34.0	40.0
32	37.84476030747921	40.0	37.0	40.0	34.0	40.0
33	37.79880890347637	40.0	37.0	40.0	34.0	40.0
34	37.74795167851325	40.0	37.0	40.0	34.0	40.0
35	37.63700522771618	40.0	37.0	40.0	33.0	40.0
36	37.22183778517103	40.0	36.0	40.0	33.0	40.0
37	37.363340474737655	40.0	36.0	40.0	33.0	40.0
38	37.31101899013393	40.0	36.0	40.0	33.0	40.0
39	37.282642758999536	40.0	36.0	40.0	33.0	40.0
40	37.21017805896339	40.0	35.0	40.0	33.0	40.0
41	37.15876882771272	40.0	35.0	40.0	33.0	40.0
42	36.931152391781005	39.0	35.0	40.0	32.0	40.0
43	36.83436994311602	39.0	35.0	40.0	32.0	40.0
44	36.729862468448715	39.0	35.0	40.0	32.0	40.0
45	36.345214061087525	39.0	35.0	40.0	31.0	40.0
46	36.38724653430611	39.0	35.0	40.0	31.0	40.0
47	36.370962540199585	38.0	35.0	40.0	31.0	40.0
48	36.24531799382038	38.0	35.0	40.0	31.0	40.0
49	36.12761472885361	38.0	35.0	40.0	31.0	40.0
50	35.92142809408098	38.0	35.0	40.0	31.0	40.0
51	35.8866297279461	37.0	35.0	40.0	31.0	40.0
52	35.39185533898629	37.0	34.0	40.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	8.0
14	13.0
15	30.0
16	95.0
17	239.0
18	663.0
19	1394.0
20	2918.0
21	5505.0
22	9514.0
23	15900.0
24	24544.0
25	36621.0
26	53024.0
27	74543.0
28	103893.0
29	138838.0
30	186207.0
31	242060.0
32	313577.0
33	414942.0
34	627310.0
35	1103920.0
36	1046629.0
37	1950719.0
38	4294468.0
39	2415682.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.802662766261125	19.232738052998574	32.796882125185164	17.167717055555133
2	30.130739983145094	19.67590471503393	34.05217397162132	16.141181330199657
3	21.593841413362686	21.336792195085806	38.040819376056064	19.028547015495448
4	20.610847662263705	21.608355404781516	39.31808124114836	18.462715691806416
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	0.0	0.0	100.0	0.0
8	0.0	0.0	100.0	0.0
9	100.0	0.0	0.0	0.0
10	0.0	0.0	100.0	0.0
11	99.38026940754516	0.5389773775406853	0.05961759766343111	0.021135617250736167
12	99.38290274776038	0.488231992986129	0.10245530651352874	0.0264099527399637
13	96.95529223684414	1.6428215413659857	0.5759513113766345	0.8259349104132301
14	52.3663202829126	26.00807746490787	13.14446313044289	8.48113912173664
15	33.76504037239717	28.491210117048144	19.136529274437457	18.607220236117225
16	29.02990425741452	19.454313728957487	25.222178511836674	26.293603501791324
17	23.46799117555446	18.81817069051003	27.593394204829625	30.12044392910589
18	23.634366222757464	20.554927457983872	29.07729672622991	26.73340959302875
19	25.973943557873813	21.54900573417487	26.617381867324514	25.859668840626803
20	29.145840122413574	20.302073211910322	24.45944376658899	26.09264289908711
21	29.202793759626715	21.453570116549034	22.984474698767695	26.359161425056556
22	28.999023750355672	21.23135907071261	24.0827077045181	25.686909474413618
23	28.569697434567807	22.025839344659605	22.994104762694327	26.41035845807826
24	27.958739539457884	23.6701765876611	23.46777683390903	24.90330703897198
25	28.80841278710202	21.682946297389694	25.272372732160136	24.23626818334815
26	28.214043404336298	20.961112531124513	24.24853158749001	26.576312477049175
27	28.89876544570776	22.121940952398013	23.880461051941335	25.098832549952892
28	29.05641372591843	22.210831494779594	23.302159637523783	25.430595141778195
29	28.462105583622826	22.075589571574685	22.949981003971672	26.512323840830813
30	27.228025905025067	21.90975803354401	23.243139134444036	27.619076926986892
31	26.03644711269173	21.063897005164943	24.000699059966436	28.898956822176892
32	27.633514367818073	23.36593393209672	22.453282515991226	26.54726918409398
33	28.835320318661722	22.613617721828486	22.121542889342223	26.42951907016757
34	27.247515684641282	22.430148928402772	24.337422129871594	25.984913257084358
35	29.154635784934797	22.33445303877892	23.478570466767973	25.03234070951831
36	30.905477860536617	21.77925459171476	23.059279933021298	24.255987614727324
37	30.79546701102183	21.546341773724578	22.84526745512241	24.812923760131184
38	28.076397792679114	23.54725165400941	25.32979332795795	23.046557225353524
39	28.638462827455662	24.30219354943411	24.66545670807824	22.393886915031985
40	28.167186789634467	25.94617100467364	22.519590635015447	23.367051570676438
41	28.61868215560637	24.97100837869147	22.51334410706304	23.89696535863912
42	29.478306979645275	24.5833332376451	21.918247493714624	24.020112288995
43	29.29281725070555	23.224315344940393	22.47022316103863	25.012644243315428
44	28.5596233772328	22.999294892537137	23.405518240971602	25.03556348925846
45	27.17274107062274	23.50299010422898	23.31897780163094	26.005291023517334
46	26.130428269152173	22.75016100502348	23.696088961581328	27.42332176424302
47	26.627846332656553	21.308062759539983	24.455371275325902	27.60871963247757
48	27.36555669080077	21.288190226985506	23.537835931728203	27.80841715048552
49	28.132011794608342	22.088526620887887	23.503051344699106	26.276410239804665
50	26.92269623111602	23.485467674715423	23.335773000561804	26.256063093606745
51	26.530940943747794	23.452803538964286	23.337916417016064	26.678339100271852
52	27.398335652433385	22.783567681474842	24.672966320726903	25.145130345364862
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	1.5
4	2.0
5	24.0
6	46.0
7	221.0
8	396.0
9	966.5
10	1537.0
11	3025.5
12	4514.0
13	5925.5
14	8725.0
15	10113.0
16	11048.5
17	11984.0
18	13085.0
19	14186.0
20	18161.5
21	22137.0
22	27400.5
23	32664.0
24	42926.0
25	53188.0
26	62517.5
27	71847.0
28	88786.5
29	105726.0
30	117796.0
31	129866.0
32	152170.5
33	174475.0
34	201697.0
35	228919.0
36	256180.0
37	283441.0
38	316510.5
39	394592.5
40	439605.0
41	487923.0
42	536241.0
43	586815.0
44	637389.0
45	706860.5
46	776332.0
47	815957.0
48	855582.0
49	879101.5
50	902621.0
51	952432.5
52	1002244.0
53	1084103.0
54	1165962.0
55	1170658.5
56	1175355.0
57	1153677.0
58	1131999.0
59	1022036.5
60	912074.0
61	792207.5
62	672341.0
63	568322.0
64	401364.0
65	338425.0
66	282822.5
67	227220.0
68	193509.5
69	159799.0
70	125822.5
71	91846.0
72	66418.5
73	40991.0
74	31748.5
75	22506.0
76	13798.5
77	5091.0
78	3572.5
79	2054.0
80	1637.0
81	1220.0
82	651.0
83	82.0
84	47.0
85	12.0
86	6.0
87	0.0
88	2.0
89	2.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1.3063257E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	5.368458975333151
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.36900574620729	3.5093082560683087
2	9.831467685305032	1.0555966187174728
3	4.8664421161196945	0.7837588456866607
4	3.0972875420273156	0.6651064441673639
5	1.9655472028193395	0.5275979761208226
6	1.2304392847435128	0.39633376930702885
7	0.8249622341792482	0.3100143137273332
8	0.6013937318196613	0.2582846061837087
9	0.4433522740932734	0.21421066455813575
>10	3.7858166511248257	4.646036987751925
>50	1.9212404620908587	7.730868344724875
>100	5.695761167458312	66.50563414961057
>500	0.34560862088463556	11.871218359845082
>1k	0.021525796429691615	1.4849264872436458
>5k	1.49484697428414E-4	0.0411041762872396
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	5.435091723296878E-4	0.0	0.0	0.0	0.0
12	0.0018372141036496488	0.0	0.0	0.0	0.0
13	0.0039193900877859175	0.0	0.0	0.0	0.0
14	0.015233566942761671	0.0	0.0	0.0	0.0
15	0.026187956035772703	0.0	0.0	0.0	0.0
16	0.03135512070228734	0.0	0.0	0.0	0.0
17	0.03623139313572411	0.0	0.0	0.0	0.0
18	0.04181193097555992	0.0	0.0	0.0	0.0
19	0.04876272433436776	1.531011753041374E-5	0.0	0.0	0.0
20	0.05742825085658194	1.531011753041374E-5	0.0	0.0	0.0
21	0.06559619855905767	1.531011753041374E-5	0.0	0.0	0.0
22	0.07518798719186187	1.531011753041374E-5	0.0	0.0	0.0
23	0.08543811087847387	1.531011753041374E-5	0.0	0.0	0.0
24	0.09502989951127808	1.531011753041374E-5	0.0	0.0	0.0
25	0.1053029883741857	1.531011753041374E-5	0.0	0.0	0.0
26	0.11597414029288408	1.531011753041374E-5	0.0	0.0	0.0
27	0.12676777315182577	1.531011753041374E-5	0.0	2.296517629562061E-5	0.0
28	0.13845704788629665	1.531011753041374E-5	0.0	2.296517629562061E-5	0.0
29	0.15105727461382717	1.531011753041374E-5	0.0	2.296517629562061E-5	0.0
30	0.16588512344203288	1.531011753041374E-5	0.0	2.296517629562061E-5	0.0
31	0.1790900998120147	1.531011753041374E-5	0.0	2.296517629562061E-5	0.0
32	0.19118509266104158	1.531011753041374E-5	0.0	2.296517629562061E-5	0.0
33	0.20193279516739202	1.531011753041374E-5	0.0	2.296517629562061E-5	0.0
34	0.21240491555819502	1.531011753041374E-5	0.0	2.296517629562061E-5	0.0
35	0.22223401101272064	1.531011753041374E-5	0.0	2.296517629562061E-5	0.0
36	0.23302764387166233	1.531011753041374E-5	0.0	2.296517629562061E-5	0.0
37	0.24341555861604805	1.531011753041374E-5	0.0	2.296517629562061E-5	0.0
38	0.2556942728754399	1.531011753041374E-5	0.0	2.296517629562061E-5	0.0
39	0.2704608812335239	1.531011753041374E-5	0.0	2.296517629562061E-5	0.0
40	0.29043292955194866	1.531011753041374E-5	0.0	2.296517629562061E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTGTG	1351570	0.0	44.19345	5
ATTGTGG	1344445	0.0	44.170704	6
TTGTGGG	1306155	0.0	44.119545	7
TTATTGT	535140	0.0	44.07538	4
ATATTGT	295565	0.0	44.074028	4
GTATTGT	281360	0.0	43.99069	4
TGTGGGG	696405	0.0	43.906765	8
GTTATTG	110805	0.0	43.897297	3
TTTATTG	203265	0.0	43.8071	3
TGTGGGA	342700	0.0	43.76443	8
ACGTATT	9505	0.0	43.749603	2
TCTATTG	106305	0.0	43.723907	3
CTATTGT	254125	0.0	43.681217	4
TATATTG	112650	0.0	43.66223	3
CGGTATT	12345	0.0	43.63386	2
TGTATTG	101440	0.0	43.59661	3
CGTATTG	57905	0.0	43.57707	3
AATATTG	74880	0.0	43.561165	3
CATATTG	57965	0.0	43.49228	3
CTTATTG	113390	0.0	43.45639	3
>>END_MODULE