FastQCFastQC Report
Fri 17 Jun 2016
SRR1527925_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527925_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13146623
Sequences flagged as poor quality0
Sequence length59
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGTG13614200.050.869755
ATTGTGG13543000.050.840946
TTGTGGG13151550.050.7720457
ATATTGT2980950.050.7446674
TTATTGT5405900.050.7127044
GTATTGT2828650.050.5838854
GTTATTG1108550.050.4684523
TGTGGGG6986350.050.418798
TCTATTG1054400.050.313313
TTTATTG2052800.050.263253
TATATTG1124750.050.224543
CTATTGT2554200.050.2194824
CGTATTG572250.050.128883
AATATTG762000.050.050923
ACGTATT94700.050.0337942
CTTATTG1158450.050.0010383
TGTATTG1019350.049.9011653
CATATTG591500.049.8863073
GTGGGGG2550950.049.8741659
CGGTATT122400.049.6441962
Produced by FastQC (version 0.11.5)