##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527925_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13146623
Sequences flagged as poor quality	0
Sequence length	59
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.166576998518934	31.0	31.0	33.0	30.0	34.0
2	31.749991309555313	31.0	31.0	34.0	30.0	34.0
3	32.04870756543335	33.0	31.0	34.0	30.0	34.0
4	35.817950130615294	37.0	35.0	37.0	35.0	37.0
5	35.406093488799364	37.0	35.0	37.0	33.0	37.0
6	35.69091096626107	37.0	35.0	37.0	33.0	37.0
7	36.09332807368097	37.0	35.0	37.0	35.0	37.0
8	36.07580494245556	37.0	35.0	37.0	35.0	37.0
9	37.39256362641569	39.0	37.0	39.0	33.0	39.0
10	36.54304325909399	37.0	35.0	39.0	32.0	39.0
11	37.71188137059989	39.0	37.0	39.0	35.0	39.0
12	37.99512407102569	39.0	38.0	39.0	35.0	39.0
13	37.944297178066186	39.0	38.0	39.0	35.0	39.0
14	38.097890614190426	40.0	38.0	40.0	34.0	40.0
15	37.58411646854101	40.0	37.0	40.0	33.0	40.0
16	37.08038505401729	39.0	36.0	40.0	32.0	40.0
17	37.04552446662538	39.0	36.0	40.0	32.0	40.0
18	37.03583528636974	39.0	36.0	40.0	32.0	40.0
19	37.24347803994988	40.0	36.0	40.0	32.0	40.0
20	37.350619623001286	40.0	36.0	40.0	32.0	40.0
21	37.289242492159396	40.0	36.0	40.0	32.0	40.0
22	37.28634494196723	40.0	36.0	40.0	32.0	40.0
23	37.24628484440453	40.0	36.0	40.0	32.0	40.0
24	37.20471728747375	40.0	36.0	40.0	32.0	40.0
25	37.19638389265441	40.0	36.0	40.0	32.0	40.0
26	37.09023640519698	40.0	36.0	40.0	32.0	40.0
27	37.00596251980451	40.0	36.0	40.0	31.0	40.0
28	37.01339416213578	39.0	36.0	40.0	31.0	40.0
29	37.002242248826946	40.0	36.0	40.0	31.0	40.0
30	36.99423000111892	39.0	36.0	40.0	31.0	40.0
31	36.999087522324174	39.0	36.0	40.0	31.0	40.0
32	36.888465729944485	39.0	35.0	40.0	31.0	40.0
33	36.7827856629037	39.0	35.0	40.0	31.0	40.0
34	36.71466740926548	39.0	35.0	40.0	31.0	40.0
35	36.6267572288336	39.0	35.0	40.0	31.0	40.0
36	36.47903396940796	39.0	35.0	40.0	30.0	40.0
37	36.39657918234972	39.0	35.0	40.0	30.0	40.0
38	36.1933442527408	38.0	35.0	40.0	30.0	40.0
39	36.10141235509682	38.0	35.0	40.0	30.0	40.0
40	35.977796046939204	38.0	35.0	40.0	30.0	40.0
41	35.92167577939978	38.0	35.0	40.0	30.0	40.0
42	35.87055786113285	38.0	35.0	40.0	29.0	40.0
43	35.715746089318905	38.0	34.0	40.0	29.0	40.0
44	35.61221562373851	38.0	34.0	40.0	29.0	40.0
45	35.40975610238462	38.0	34.0	40.0	28.0	40.0
46	35.24558900030829	38.0	34.0	40.0	28.0	40.0
47	35.18666725287551	37.0	34.0	40.0	28.0	40.0
48	35.0321216330612	37.0	34.0	40.0	27.0	40.0
49	34.93700138811313	37.0	34.0	40.0	27.0	40.0
50	34.742147698310056	37.0	33.0	40.0	27.0	40.0
51	34.60649035117231	36.0	33.0	39.0	27.0	40.0
52	34.45661231785532	36.0	33.0	39.0	26.0	40.0
53	34.269863294931326	36.0	33.0	39.0	26.0	40.0
54	34.134453768089344	35.0	33.0	39.0	26.0	40.0
55	33.99785732046929	35.0	33.0	39.0	26.0	40.0
56	33.79491493747101	35.0	33.0	39.0	25.0	40.0
57	33.64665290850738	35.0	33.0	38.0	25.0	40.0
58	33.47552546383965	35.0	32.0	38.0	25.0	40.0
59	32.85127990663458	35.0	31.0	38.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	6.0
13	20.0
14	33.0
15	157.0
16	527.0
17	1487.0
18	3712.0
19	7508.0
20	13604.0
21	22167.0
22	33686.0
23	48089.0
24	67192.0
25	90024.0
26	119336.0
27	154523.0
28	195893.0
29	244943.0
30	306442.0
31	383994.0
32	481997.0
33	617956.0
34	886822.0
35	1222598.0
36	1428431.0
37	2218480.0
38	3641192.0
39	955802.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.99491025185707	19.4049148591239	32.36282808140159	17.237346807617442
2	29.63440116903025	20.114937501440483	33.99916465239781	16.251496677131456
3	21.656185014204787	21.45201851456454	37.89041489970466	19.00138157152601
4	20.572697642580913	21.76699674129242	39.16452156572832	18.495784050398342
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	0.0	0.0	100.0	0.0
8	0.0	0.0	100.0	0.0
9	100.0	0.0	0.0	0.0
10	0.0	0.0	100.0	0.0
11	99.36634677970152	0.5478593247862968	0.06177251755070484	0.024021377961473453
12	99.37215055151427	0.4956177719555813	0.09894556191350433	0.03328611461665859
13	96.93736558810578	1.6640927483810863	0.573614988427066	0.8249266750860659
14	52.03483814816931	26.185568719814967	13.197016450536387	8.582576681479342
15	33.62913046186842	28.65592175268128	19.143920077422163	18.571027708028137
16	28.978696658449856	19.595305958039567	25.179842762662318	26.246154620848262
17	23.47446184468818	18.95690627167144	27.48681543541638	30.081816448224004
18	23.57432018853815	20.664287703389682	29.055172571693888	26.706219536378278
19	25.925897471921118	21.696104010893137	26.63353927468674	25.74445924249901
20	29.118184951374964	20.450453321738973	24.478993578807273	25.952368148078786
21	29.119599763376495	21.605822270859974	23.095383506471588	26.179194459291942
22	28.902950970754997	21.35738584730086	24.171363246667983	25.568299935276155
23	28.526010063572983	22.18489113135746	23.010350262573134	26.278748542496427
24	27.87030555299258	23.816937627252262	23.526779462680263	24.78597735707489
25	28.67988988502979	21.85732412042241	25.355020829303466	24.107765165244338
26	28.132585835921514	21.034276254822245	24.369064207591563	26.46407370166468
27	28.774796386874407	22.22097644391263	24.005617260037045	24.998609909175915
28	28.885311459832693	22.337006241070426	23.41420302384879	25.363479275248103
29	28.3867119335513	22.164064490173637	23.019074936582573	26.43014863969249
30	27.12540703418665	22.00908172387692	23.299603251724797	27.56590799021163
31	25.875983513028405	21.171429347293216	24.050092559891617	28.902494579786765
32	27.47647817998584	23.546913910895597	22.519037778751244	26.45757013036732
33	28.76339421918465	22.741726145185726	22.177938775608002	26.31694086002162
34	27.15921799841678	22.54018389361283	24.44124243921804	25.85935566875235
35	29.077102157717615	22.468720674503253	23.505717019496185	24.948460148282948
36	30.731709580475535	21.91562806661452	23.14663621220446	24.20602614070549
37	30.73885970564456	21.64170981399558	22.876962395590105	24.742468084769754
38	27.9126053892319	23.69130840672924	25.398028071543543	22.99805813249532
39	28.493355289795712	24.46389464427481	24.72548273423525	22.31726733169423
40	28.039208243820486	26.13677291879443	22.577045070814002	23.24697376657108
41	28.478530189844193	25.15569207392651	22.556416199049746	23.809361537179548
42	29.279199684968525	24.763743510405675	21.960902050663506	23.99615475396229
43	29.1822698498314	23.35329004262159	22.513728430487433	24.950711677059576
44	28.248935106757074	23.15748310421619	23.559114762779764	25.034467026246972
45	27.015127763228623	23.574479925377034	23.373401671288512	26.036990640105827
46	26.03574317145932	22.847745767106883	23.71868425830725	27.397826803126552
47	26.528926858250973	21.4197973122071	24.484211648877434	27.567064180664495
48	27.216959062414738	21.39975414218541	23.556779562325623	27.826507233074228
49	27.962382430834136	22.26054554085867	23.52525815945281	26.251813868854384
50	26.72879567627367	23.656295612949425	23.320939529489817	26.293969181287085
51	26.392344254490297	23.549058948446305	23.44316863729948	26.615428159763923
52	27.246624475350057	22.89823021470989	24.794975865665275	25.060169444274777
53	27.631879304670104	23.199699268777998	23.807558792855016	25.36086263369688
54	26.023960677962698	22.889551179797277	24.172808484734066	26.913679657505963
55	25.795285983328192	23.124478430696612	24.45590780233068	26.624327783644514
56	25.934629752446693	22.33757672978072	24.91963905863886	26.808154459133725
57	25.57374620083043	22.469998569214315	25.922824439401666	26.03343079055359
58	25.613398969453982	23.18213582301706	25.056533529561165	26.147931677967794
59	25.945157170780664	22.61919277673057	23.860964142654733	27.57468590983403
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	18.5
5	37.0
6	184.5
7	332.0
8	747.5
9	2409.5
10	3656.0
11	4719.5
12	5783.0
13	6589.0
14	7571.5
15	7748.0
16	8609.5
17	9471.0
18	10265.0
19	13183.0
20	15307.0
21	18418.5
22	21530.0
23	27192.0
24	32854.0
25	41934.0
26	60203.0
27	69392.0
28	81393.0
29	93394.0
30	110710.0
31	146133.0
32	164240.0
33	179679.5
34	195119.0
35	212288.5
36	250376.0
37	271294.0
38	305288.5
39	339283.0
40	378845.5
41	418408.0
42	452365.0
43	508039.0
44	529756.0
45	578460.5
46	627165.0
47	652488.0
48	716389.0
49	754967.0
50	794390.0
51	833813.0
52	886186.0
53	989873.5
54	1041188.0
55	1069061.5
56	1096935.0
57	1041772.0
58	907337.5
59	828066.0
60	736742.5
61	645419.0
62	572815.5
63	500212.0
64	432702.0
65	321325.5
66	277459.0
67	238974.5
68	200490.0
69	160618.0
70	97996.0
71	75246.0
72	58875.5
73	42505.0
74	32005.0
75	17115.5
76	12726.0
77	8620.5
78	4515.0
79	3318.0
80	2121.0
81	1468.5
82	561.5
83	307.0
84	222.0
85	137.0
86	96.5
87	36.5
88	17.0
89	8.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	1.3146623E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.120345683321109
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.94760354699419	6.926683981684115
2	8.206402861580298	1.4969046182841572
3	3.717931571460852	1.0172646347596874
4	2.120707538670547	0.7736634338360184
5	1.297923165722737	0.5918753970890916
6	0.8053384196444416	0.4406978867530097
7	0.5614746575370813	0.3584590078413762
8	0.3921970427927849	0.2861578084997184
9	0.28307265334089704	0.23235484067675127
>10	2.1806671396951733	4.493392995331239
>50	1.066903454849966	7.304574032616834
>100	3.216223650933445	63.46720223581854
>500	0.1914018087272181	11.168406945676226
>1k	0.012068095772169324	1.403300578595101
>5k	8.439227812691721E-5	0.039061602538163535
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	5.248496134710792E-4	0.0	0.0	0.0	0.0
12	0.0019320550988645524	0.0	0.0	0.0	0.0
13	0.003757618971807437	0.0	0.0	0.0	0.0
14	0.014292643821915332	0.0	0.0	0.0	0.0
15	0.024249573445591314	0.0	0.0	0.0	0.0
16	0.028828696160223048	0.0	0.0	0.0	0.0
17	0.033407818874854785	0.0	0.0	0.0	0.0
18	0.0387323801709382	0.0	0.0	0.0	0.0
19	0.04514467327465008	1.5213032274524036E-5	0.0	0.0	0.0
20	0.05300220444444174	1.5213032274524036E-5	0.0	0.0	0.0
21	0.060661966194664595	1.5213032274524036E-5	0.0	0.0	0.0
22	0.06932578807500603	1.5213032274524036E-5	0.0	7.606516137262018E-6	0.0
23	0.07871222898838735	1.5213032274524036E-5	0.0	7.606516137262018E-6	0.0
24	0.0872163140298463	1.5213032274524036E-5	0.0	7.606516137262018E-6	0.0
25	0.09637455945910976	1.5213032274524036E-5	0.0	7.606516137262018E-6	0.0
26	0.10661293017986444	1.5213032274524036E-5	0.0	7.606516137262018E-6	0.0
27	0.1161362883837165	1.5213032274524036E-5	0.0	7.606516137262018E-6	0.0
28	0.1270440325245502	1.5213032274524036E-5	0.0	7.606516137262018E-6	0.0
29	0.13875806737593374	1.5213032274524036E-5	0.0	7.606516137262018E-6	0.0
30	0.15210750319682856	1.5213032274524036E-5	0.0	7.606516137262018E-6	0.0
31	0.16518310443678197	1.5213032274524036E-5	0.0	7.606516137262018E-6	0.0
32	0.17658527212653774	1.5213032274524036E-5	0.0	7.606516137262018E-6	0.0
33	0.18642049749201753	1.5213032274524036E-5	0.0	7.606516137262018E-6	0.0
34	0.19570805369561445	1.5213032274524036E-5	0.0	7.606516137262018E-6	0.0
35	0.204607677576211	1.5213032274524036E-5	0.0	7.606516137262018E-6	0.0
36	0.21375070997319995	1.5213032274524036E-5	0.0	7.606516137262018E-6	0.0
37	0.22331210075773833	1.5213032274524036E-5	0.0	7.606516137262018E-6	0.0
38	0.23488161180251385	1.5213032274524036E-5	0.0	7.606516137262018E-6	0.0
39	0.24869504510778168	1.5213032274524036E-5	0.0	7.606516137262018E-6	0.0
40	0.26664642319172005	1.5213032274524036E-5	0.0	7.606516137262018E-6	0.0
41	0.28192791411147944	1.5213032274524036E-5	0.0	7.606516137262018E-6	0.0
42	0.294775319867315	1.5213032274524036E-5	0.0	7.606516137262018E-6	0.0
43	0.30604817678273727	1.5213032274524036E-5	0.0	7.606516137262018E-6	7.606516137262018E-6
44	0.3165299560198844	1.5213032274524036E-5	0.0	7.606516137262018E-6	7.606516137262018E-6
45	0.32714865254750214	1.5213032274524036E-5	0.0	7.606516137262018E-6	7.606516137262018E-6
46	0.3382769856563165	1.5213032274524036E-5	0.0	7.606516137262018E-6	7.606516137262018E-6
47	0.34897174734530684	1.5213032274524036E-5	0.0	7.606516137262018E-6	7.606516137262018E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTGTG	1361420	0.0	50.86975	5
ATTGTGG	1354300	0.0	50.84094	6
TTGTGGG	1315155	0.0	50.772045	7
ATATTGT	298095	0.0	50.744667	4
TTATTGT	540590	0.0	50.712704	4
GTATTGT	282865	0.0	50.583885	4
GTTATTG	110855	0.0	50.468452	3
TGTGGGG	698635	0.0	50.41879	8
TCTATTG	105440	0.0	50.31331	3
TTTATTG	205280	0.0	50.26325	3
TATATTG	112475	0.0	50.22454	3
CTATTGT	255420	0.0	50.219482	4
CGTATTG	57225	0.0	50.12888	3
AATATTG	76200	0.0	50.05092	3
ACGTATT	9470	0.0	50.033794	2
CTTATTG	115845	0.0	50.001038	3
TGTATTG	101935	0.0	49.901165	3
CATATTG	59150	0.0	49.886307	3
GTGGGGG	255095	0.0	49.874165	9
CGGTATT	12240	0.0	49.644196	2
>>END_MODULE