FastQCFastQC Report
Fri 17 Jun 2016
SRR1527924_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527924_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3738432
Sequences flagged as poor quality0
Sequence length52
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGTA551.8189894E-1246.028
CGAACTA800.046.016
TTTACGG1200.046.029
GCGAATA301.862536E-646.036
ACGTTAC206.3132896E-446.017
CTATAGT253.4186436E-546.017
CTATACG206.3132896E-446.024
TAGCGAA253.4186436E-546.040
ATAGCGG1000.046.020
CGTCTAC405.6170393E-946.032
CACGACG453.110472E-1046.038
ATCGTTC551.8189894E-1246.040
ACGATTA206.3132896E-446.040
TACGCTA206.3132896E-446.024
CTAACGT206.3132896E-446.023
TACGCGC1050.046.043
CGTAGTG206.3132896E-446.034
CTACGTC405.6170393E-946.038
GTACTAG405.6170393E-946.023
TACCTAG253.4186436E-546.031
Produced by FastQC (version 0.11.5)