##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527924_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3738432
Sequences flagged as poor quality	0
Sequence length	52
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.804535430897232	33.0	31.0	34.0	30.0	34.0
2	32.31480337210895	34.0	31.0	34.0	30.0	34.0
3	32.43449686927568	34.0	31.0	34.0	30.0	34.0
4	36.03678360339308	37.0	35.0	37.0	35.0	37.0
5	35.986979835396234	37.0	35.0	37.0	35.0	37.0
6	36.0146160208344	37.0	35.0	37.0	35.0	37.0
7	36.00962756578159	37.0	35.0	37.0	35.0	37.0
8	35.973470428243715	37.0	35.0	37.0	35.0	37.0
9	37.5489550164347	39.0	37.0	39.0	35.0	39.0
10	37.890720762073514	39.0	38.0	39.0	35.0	39.0
11	38.209644845753516	39.0	39.0	39.0	37.0	39.0
12	38.1441922709842	39.0	39.0	39.0	37.0	39.0
13	38.108361205981545	39.0	39.0	39.0	37.0	39.0
14	38.70564851788129	40.0	39.0	40.0	36.0	40.0
15	38.14268334959684	40.0	38.0	40.0	34.0	40.0
16	37.604712349990585	40.0	37.0	40.0	33.0	40.0
17	37.651970398284625	40.0	37.0	40.0	33.0	40.0
18	37.335701170972214	40.0	36.0	40.0	32.0	40.0
19	37.72511389801927	40.0	37.0	40.0	33.0	40.0
20	37.79417467002209	40.0	37.0	40.0	33.0	40.0
21	37.67298616104292	40.0	37.0	40.0	33.0	40.0
22	37.767471228579254	40.0	37.0	40.0	33.0	40.0
23	37.69803757297177	40.0	37.0	40.0	33.0	40.0
24	37.65607158295243	40.0	37.0	40.0	33.0	40.0
25	37.840042563299264	40.0	37.0	40.0	33.0	40.0
26	37.81660519704518	40.0	38.0	40.0	33.0	40.0
27	37.73841252161334	40.0	37.0	40.0	33.0	40.0
28	37.68922853217606	40.0	37.0	40.0	33.0	40.0
29	37.66017597752213	40.0	37.0	40.0	33.0	40.0
30	37.54631406964203	40.0	37.0	40.0	33.0	40.0
31	37.47730920343074	40.0	36.0	40.0	33.0	40.0
32	37.35165866331125	40.0	36.0	40.0	33.0	40.0
33	37.26765553044699	40.0	36.0	40.0	33.0	40.0
34	37.17409304221663	40.0	36.0	40.0	32.0	40.0
35	37.119650163491	40.0	36.0	40.0	32.0	40.0
36	37.00970995326383	40.0	35.0	40.0	32.0	40.0
37	36.881144019738755	39.0	35.0	40.0	32.0	40.0
38	36.71504978557855	39.0	35.0	40.0	31.0	40.0
39	36.56316257725164	39.0	35.0	40.0	31.0	40.0
40	36.37599801200075	39.0	35.0	40.0	31.0	40.0
41	36.16737872990601	39.0	35.0	40.0	30.0	40.0
42	35.89580069933063	38.0	35.0	40.0	30.0	40.0
43	35.588141498895794	38.0	35.0	40.0	29.0	40.0
44	35.327661971650144	38.0	35.0	40.0	28.0	40.0
45	35.09482344469553	37.0	35.0	40.0	27.0	40.0
46	34.885542655316456	37.0	34.0	40.0	27.0	40.0
47	34.699761022803145	37.0	34.0	40.0	26.0	40.0
48	34.47731615821821	36.0	34.0	40.0	25.0	40.0
49	34.27645601150429	36.0	34.0	40.0	24.0	40.0
50	34.05751341738996	36.0	33.0	40.0	24.0	40.0
51	33.84089024489412	35.0	33.0	39.0	23.0	40.0
52	33.16042367495249	35.0	33.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	3.0
13	6.0
14	11.0
15	17.0
16	54.0
17	144.0
18	391.0
19	892.0
20	1978.0
21	3550.0
22	5881.0
23	9057.0
24	13478.0
25	19664.0
26	27298.0
27	36752.0
28	49068.0
29	63378.0
30	79573.0
31	94187.0
32	107873.0
33	131074.0
34	178833.0
35	304114.0
36	321306.0
37	609302.0
38	1145366.0
39	535180.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.649648836731547	20.69450507592488	37.085976152568776	15.569869934774793
2	19.33235645318679	22.048361452074026	38.41848668104703	20.20079541369216
3	23.705366314005445	23.839245972643074	32.11632577508431	20.339061938267168
4	26.06047134199579	27.7071242702823	36.398682656258025	9.833721731463886
5	0.0	0.0	0.0	100.0
6	0.0	0.0	100.0	0.0
7	0.0	0.0	0.0	100.0
8	0.0	0.0	0.0	100.0
9	0.0	100.0	0.0	0.0
10	0.0	0.0	100.0	0.0
11	99.08750513584305	0.6222127351788129	0.12871706640645061	0.1615650625716878
12	99.00626786845395	0.6240316795918717	0.22587009740982317	0.14383035454436513
13	96.59210064540427	2.4440995583174976	0.39187552428397787	0.5719242719942478
14	53.50938575317138	33.222270727406574	5.504420034923733	7.763923484498314
15	38.04116271206752	27.040133403523186	15.930769905671681	18.98793397873761
16	27.231871544005614	29.87875665519662	22.8082789789944	20.081092821803363
17	30.892122686730694	18.511290294968585	26.19156908564874	24.40501793265198
18	20.4188280006163	19.609558231900433	36.368937565268006	23.60267620221526
19	23.88597679454916	21.39939953435023	25.60926613082704	29.10535754027357
20	27.570863934398165	19.650965966480065	25.22857176484687	27.549598334274904
21	29.64076917809392	22.711928423467377	20.933428774416655	26.71387362402205
22	27.940805128995255	23.741076472702993	21.390304812284935	26.927813586016814
23	23.336842826083235	20.39063436221389	20.417062554568332	35.85546025713454
24	24.550667231609403	21.70107681509253	28.447595141492478	25.300660811805592
25	26.76675675791348	18.299998502045778	20.15936092992998	34.77388381011076
26	27.47582408881585	20.764079699724377	16.52297005803503	35.237126153424754
27	27.415718675637272	32.29707000154075	17.790373076198794	22.49683824662318
28	27.277719642887714	22.550764598633865	27.27309203430743	22.89842372417099
29	37.42454055604061	20.812843459503878	17.525582918186018	24.237033066269493
30	38.41720272028486	20.654461549655043	17.03452142502525	23.893814305034837
31	34.92568542105353	20.71071508054714	18.373558754044474	25.990040744354854
32	31.32037709927585	27.54866211288583	17.13905723041104	23.991903557427285
33	28.79894565422081	28.663701787273382	18.436900818310992	24.10045174019482
34	25.977495377741256	31.00620795028504	18.58246986972078	24.433826802252923
35	28.626092436615135	29.02187334155068	17.757257588208105	24.594776633626076
36	28.283114418023388	28.115691284474348	19.90976430760276	23.69142998989951
37	28.016772807423006	29.631299967472994	19.878762005033128	22.473165220070875
38	27.379714275931725	29.546371312892678	20.8151171400202	22.25879727115539
39	28.777733552462635	30.391377989488642	19.49317253864722	21.337715919401504
40	27.822707488059166	31.202948187903377	17.974621445568623	22.999722878468834
41	27.626528983274273	29.752687757862116	17.840046308184824	24.780736950678786
42	29.41990117782001	29.268688048893228	18.509712093198434	22.801698680088336
43	28.016665810692825	29.661419547018642	17.151656095389725	25.170258546898804
44	27.595446433157	29.34834711451218	17.647933679146767	25.408272773184056
45	26.19015137897386	31.286806875181895	18.15975253796244	24.36328920788181
46	25.693365560748465	28.89668716724017	21.5245589594782	23.885388312533166
47	28.758099652474623	26.92297198397617	20.65702947117936	23.661898892369848
48	28.49783010631195	27.15194498656121	20.31271399346036	24.03751091366648
49	27.607296321024428	27.73681586290723	20.372177426257853	24.283710389810487
50	27.31613146902231	28.899388834677215	19.97356110797254	23.81091858832794
51	26.016361939979117	29.98628836902744	20.073897291698767	23.92345239929468
52	27.68296975844418	29.521628319038566	19.98682870251485	22.808573220002394
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.5
6	3.0
7	12.5
8	22.0
9	27.0
10	32.0
11	198.0
12	364.0
13	683.0
14	2009.0
15	3016.0
16	3561.0
17	4106.0
18	4586.5
19	5067.0
20	5918.5
21	6770.0
22	8665.0
23	10560.0
24	13355.5
25	16151.0
26	20617.5
27	25084.0
28	31064.0
29	37044.0
30	46726.5
31	56409.0
32	68596.0
33	80783.0
34	84947.0
35	89111.0
36	85848.0
37	82585.0
38	76450.0
39	66533.0
40	62751.0
41	67967.0
42	73183.0
43	78624.5
44	84066.0
45	93940.5
46	103815.0
47	115980.0
48	128145.0
49	152975.5
50	177806.0
51	199181.0
52	220556.0
53	238746.5
54	256937.0
55	265505.5
56	274074.0
57	293887.0
58	313700.0
59	310093.0
60	306486.0
61	309857.5
62	313229.0
63	287220.0
64	229309.0
65	197407.0
66	169040.0
67	140673.0
68	127026.5
69	113380.0
70	102832.5
71	92285.0
72	77453.0
73	62621.0
74	50198.5
75	37776.0
76	27936.5
77	18097.0
78	13773.0
79	9449.0
80	5612.0
81	1775.0
82	1055.0
83	335.0
84	283.0
85	231.0
86	123.0
87	15.0
88	10.0
89	2.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	3738432.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.991651139897245
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.36555226110258	12.212112708651437
2	8.134391664196833	2.6016470745824787
3	3.6559438491387417	1.7539373586743967
4	2.3552747565015832	1.50658928978319
5	1.5490945972518255	1.2386290190975413
6	1.1555992566026267	1.1087964101468284
7	0.8625773856264328	0.965582564247177
8	0.6917959838813751	0.8850368027370341
9	0.5409260468068636	0.7785270569717181
>10	3.056207875699446	8.546934298423366
>50	0.2829201657345276	3.292349351932321
>100	1.195994394378233	47.52087787209449
>500	0.13939032234787652	14.687272788657285
>1k	0.014331440730993423	2.9017074040007507
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.005884820159895913	0.0	0.0	0.0	0.0
12	0.009335464708198517	0.0	0.0	0.0	0.0
13	0.02420801020320819	0.0	0.0	0.0	0.0
14	0.15886339513464467	0.0	0.0	0.0	0.0
15	0.24748343690616814	0.0	0.0	0.0	0.0
16	0.31965273141252804	0.0	0.0	0.0	0.0
17	0.3742745621693801	0.0	0.0	0.0	0.0
18	0.4500549963193125	0.0	0.0	0.0	0.0
19	0.5111501292520501	0.0	0.0	0.0	0.0
20	0.5624550613733245	0.0	0.0	0.0	0.0
21	0.6303177374899424	0.0	0.0	0.0	0.0
22	0.671136990053584	0.0	0.0	0.0	0.0
23	0.714310170681184	0.0	0.0	0.0	8.024754763494428E-5
24	0.7793641826305788	0.0	0.0	0.0	8.024754763494428E-5
25	0.8276464571242703	0.0	0.0	0.0	8.024754763494428E-5
26	0.8852909455087052	0.0	0.0	0.0	8.024754763494428E-5
27	0.9680796654854228	0.0	0.0	0.0	8.024754763494428E-5
28	1.0487017016759967	0.0	0.0	0.0	8.024754763494428E-5
29	1.1066136818858816	0.0	0.0	0.0	8.024754763494428E-5
30	1.1731923972403404	0.0	0.0	0.0	8.024754763494428E-5
31	1.2368287025148512	0.0	0.0	0.0	8.024754763494428E-5
32	1.3780911355348981	0.0	0.0	0.0	8.024754763494428E-5
33	1.4744149418793762	0.0	0.0	0.0	8.024754763494428E-5
34	1.5737346566688921	0.0	0.0	0.0	8.024754763494428E-5
35	1.6430150394603942	0.0	0.0	0.0	8.024754763494428E-5
36	1.7145423535856745	0.0	0.0	0.0	8.024754763494428E-5
37	1.7845717134884358	0.0	0.0	0.0	8.024754763494428E-5
38	1.8551895554071867	0.0	0.0	0.0	8.024754763494428E-5
39	1.9303280091760395	0.0	0.0	0.0	8.024754763494428E-5
40	2.013170227517847	0.0	0.0	0.0	8.024754763494428E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGTA	55	1.8189894E-12	46.0	28
CGAACTA	80	0.0	46.0	16
TTTACGG	120	0.0	46.0	29
GCGAATA	30	1.862536E-6	46.0	36
ACGTTAC	20	6.3132896E-4	46.0	17
CTATAGT	25	3.4186436E-5	46.0	17
CTATACG	20	6.3132896E-4	46.0	24
TAGCGAA	25	3.4186436E-5	46.0	40
ATAGCGG	100	0.0	46.0	20
CGTCTAC	40	5.6170393E-9	46.0	32
CACGACG	45	3.110472E-10	46.0	38
ATCGTTC	55	1.8189894E-12	46.0	40
ACGATTA	20	6.3132896E-4	46.0	40
TACGCTA	20	6.3132896E-4	46.0	24
CTAACGT	20	6.3132896E-4	46.0	23
TACGCGC	105	0.0	46.0	43
CGTAGTG	20	6.3132896E-4	46.0	34
CTACGTC	40	5.6170393E-9	46.0	38
GTACTAG	40	5.6170393E-9	46.0	23
TACCTAG	25	3.4186436E-5	46.0	31
>>END_MODULE