FastQCFastQC Report
Fri 17 Jun 2016
SRR1527923_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527923_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3709609
Sequences flagged as poor quality0
Sequence length52
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGTA700.046.00000437
ACGCCTA351.0203985E-746.00000429
CGCATGA351.0203985E-746.00000420
TTAACGA351.0203985E-746.00000421
AATAGCG850.046.00000420
GCGTATA850.046.00000424
TAATCCG351.0203985E-746.00000427
ACACGTA405.6170393E-946.019
CCGATTA453.110472E-1046.027
CCGATCA206.3132826E-446.035
ACGTTAG206.3132826E-446.041
ACACCGT800.046.028
CTATAGT453.110472E-1046.017
CTATACG453.110472E-1046.024
ATTGTCG501.6370905E-1146.035
CACGAAT253.4186392E-546.035
TACGGAC453.110472E-1046.015
ATCGTAG206.3132826E-446.039
TAACCGT253.4186392E-546.041
CTTATAG206.3132826E-446.024
Produced by FastQC (version 0.11.5)