FastQCFastQC Report
Fri 17 Jun 2016
SRR1527922_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527922_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3801710
Sequences flagged as poor quality0
Sequence length59
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATTA203.1391668E-453.00000447
CGTTAGT203.1391668E-453.00000418
TACTAAC203.1391668E-453.00000450
CACGTAA203.1391668E-453.00000450
CGTTTAG203.1391668E-453.00000415
ACTATCG150.006697450353.036
GCGAATA150.006697450353.036
TTCGTTA307.0148053E-753.019
TACGGGT251.4793839E-553.037
CTTATAC307.0148053E-753.046
TACGATT307.0148053E-753.029
TATTCGC251.4793839E-553.015
CGATCAA1450.053.041
GCGTATA1050.053.024
GCGTAAT307.0148053E-753.014
TACGTAA353.3447577E-853.018
ACGGTTA251.4793839E-553.026
CAATGCG2700.051.03703344
CGATACA2350.050.7446850
AACGATA2300.050.69565248
Produced by FastQC (version 0.11.5)