##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527922_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3801710
Sequences flagged as poor quality	0
Sequence length	59
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.39159299367916	31.0	31.0	34.0	30.0	34.0
2	31.89298315757909	31.0	31.0	34.0	30.0	34.0
3	32.088652737846914	33.0	31.0	34.0	30.0	34.0
4	35.815727396355854	37.0	35.0	37.0	35.0	37.0
5	35.668612808446724	37.0	35.0	37.0	33.0	37.0
6	35.73435927516828	37.0	35.0	37.0	33.0	37.0
7	35.72935889376097	37.0	35.0	37.0	33.0	37.0
8	35.704440896333494	37.0	35.0	37.0	33.0	37.0
9	37.21659595287384	39.0	37.0	39.0	33.0	39.0
10	37.58061793245671	39.0	37.0	39.0	35.0	39.0
11	37.94429348898259	39.0	37.0	39.0	35.0	39.0
12	37.89363418040829	39.0	37.0	39.0	35.0	39.0
13	37.868391855244084	39.0	38.0	39.0	35.0	39.0
14	38.230131440851615	40.0	38.0	40.0	34.0	40.0
15	37.64967422554587	40.0	37.0	40.0	33.0	40.0
16	37.16555260659019	39.0	36.0	40.0	32.0	40.0
17	37.13995649326224	39.0	36.0	40.0	32.0	40.0
18	36.7619823710909	39.0	36.0	40.0	31.0	40.0
19	37.138330908985694	39.0	36.0	40.0	32.0	40.0
20	37.28997398539079	39.0	36.0	40.0	32.0	40.0
21	37.318962782537334	40.0	36.0	40.0	32.0	40.0
22	37.313980813896904	40.0	36.0	40.0	32.0	40.0
23	37.269157826346564	40.0	36.0	40.0	32.0	40.0
24	37.245521094454865	40.0	36.0	40.0	32.0	40.0
25	37.22509528606864	40.0	36.0	40.0	32.0	40.0
26	37.06130320303232	40.0	36.0	40.0	31.0	40.0
27	36.89204226519119	39.0	36.0	40.0	31.0	40.0
28	36.86090127863514	39.0	36.0	40.0	31.0	40.0
29	36.79370125548766	39.0	35.0	40.0	31.0	40.0
30	36.66671603041789	39.0	35.0	40.0	31.0	40.0
31	36.6347567278935	39.0	35.0	40.0	30.0	40.0
32	36.428204939356235	39.0	35.0	40.0	30.0	40.0
33	36.271352628159434	39.0	35.0	40.0	30.0	40.0
34	36.19222034295093	39.0	35.0	40.0	30.0	40.0
35	36.10506929776337	39.0	35.0	40.0	30.0	40.0
36	35.8881782145403	38.0	35.0	40.0	29.0	40.0
37	35.764147712476756	38.0	35.0	40.0	29.0	40.0
38	35.59261516528089	38.0	35.0	40.0	28.0	40.0
39	35.417700718887026	38.0	34.0	40.0	27.0	40.0
40	35.18865957687462	38.0	34.0	40.0	26.0	40.0
41	34.99785017794624	38.0	34.0	40.0	26.0	40.0
42	34.75752648150438	37.0	34.0	40.0	25.0	40.0
43	34.408934926651426	37.0	33.0	40.0	24.0	40.0
44	34.15639856801282	37.0	33.0	40.0	24.0	40.0
45	33.877041910087826	36.0	33.0	40.0	23.0	40.0
46	33.59934108598499	36.0	33.0	39.0	23.0	40.0
47	33.42983157579089	35.0	33.0	39.0	22.0	40.0
48	33.162723353438324	35.0	32.0	39.0	20.0	40.0
49	33.01359940658283	35.0	32.0	39.0	20.0	40.0
50	32.74290516635935	35.0	32.0	39.0	20.0	40.0
51	32.52308776839896	35.0	31.0	38.0	20.0	40.0
52	32.26403039684774	35.0	31.0	38.0	18.0	40.0
53	31.97523535461674	35.0	31.0	38.0	18.0	40.0
54	31.682009148514748	35.0	30.0	38.0	16.0	40.0
55	31.407243319453613	35.0	30.0	37.0	15.0	40.0
56	31.06346170539047	35.0	29.0	37.0	13.0	40.0
57	30.757746119509378	34.0	29.0	37.0	11.0	39.0
58	30.36982226419164	34.0	28.0	36.0	10.0	39.0
59	29.739700818842046	34.0	27.0	36.0	10.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	4.0
13	3.0
14	20.0
15	58.0
16	233.0
17	681.0
18	1913.0
19	4037.0
20	7526.0
21	11929.0
22	18053.0
23	25500.0
24	35005.0
25	46575.0
26	59677.0
27	74119.0
28	87902.0
29	96902.0
30	108613.0
31	129006.0
32	157225.0
33	196687.0
34	264180.0
35	358945.0
36	434310.0
37	660302.0
38	841782.0
39	180521.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.758695429162138	21.146115826825294	36.51456844420011	15.580620299812454
2	19.00302758495519	22.589597838867245	38.20052029218431	20.206854283993255
3	23.57917884320477	24.071772965323497	31.995286331677057	20.353761859794673
4	26.062955880380144	27.944845871989184	36.16980253622712	9.822395711403553
5	0.0	0.0	0.0	100.0
6	0.0	0.0	100.0	0.0
7	0.0	0.0	0.0	100.0
8	0.0	0.0	0.0	100.0
9	0.0	100.0	0.0	0.0
10	0.0	0.0	100.0	0.0
11	99.0767575643592	0.6261918978564909	0.12812655357720604	0.1689239842071068
12	98.99973959086832	0.6212204507971413	0.22129515402279498	0.15774480431174392
13	96.56415139503012	2.46628490863322	0.38527399512324717	0.5842897012134013
14	53.19624589987137	33.495663793398236	5.553343100867767	7.754747205862625
15	38.135733656696594	27.18979090988003	15.904132614007906	18.770342819415472
16	27.251421071044348	30.183943541195934	22.61248227771187	19.952153110047846
17	31.078856619784258	18.54397100252255	26.03917710714389	24.337995270549307
18	20.392086718871244	19.720231159136283	36.40411814683392	23.483563975158546
19	23.85473905163729	21.502455473984075	25.610054422878125	29.03275105150051
20	27.55917731757551	19.706211152349866	25.278966570306522	27.455644959768104
21	29.33348414266212	22.93020772231443	21.032298623514155	26.704009511509298
22	27.849862298807643	23.897509278719316	21.471548329567483	26.78108009290556
23	23.15463304670793	20.448219353922315	20.51345315660584	35.88369444276391
24	24.405990988265806	21.790431148088622	28.655341938233057	25.14823592541251
25	26.591481201880207	18.330409210592073	20.167266835187323	34.91084275234039
26	27.228036857098516	20.856325179984797	16.531245150208722	35.384392812707965
27	27.284827090966964	32.5752095767431	17.88581980214167	22.254143530148276
28	27.163644780901226	22.631184388077997	27.509252415360457	22.695918415660323
29	37.613494979890625	20.86342724721244	17.444044916629622	24.07903285626731
30	38.55144132508792	20.7820165136215	16.98869719152697	23.677844969763605
31	35.000802270557195	20.861664882381874	18.30197463772881	25.835558209332117
32	31.151139881790037	27.97022918633983	17.056298349953046	23.822332581917085
33	28.750693766752327	29.0908564830037	18.28713920840885	23.871310541835122
34	25.84831562638918	31.40626191897857	18.50756633199271	24.23785612263955
35	28.449566116300296	29.406951082539173	17.70106083841219	24.44242196274834
36	28.023784034026793	28.466979332984366	19.97761533625658	23.53162129673226
37	27.725497210465814	30.07596581538308	19.834390313832458	22.364146660318646
38	27.04148922458578	29.957861067782655	20.85587801278898	22.144771694842582
39	28.38304341993471	30.89375570466974	19.387091598254468	21.336109277141077
40	27.56670024804627	31.793114151263513	17.884899163797343	22.75528643689287
41	27.37281381273164	30.1590336979938	17.727338487154466	24.7408140021201
42	29.114267000902228	29.774390997735228	18.46221831754658	22.649123683815965
43	27.719394693440584	30.12460182391608	17.071738770184997	25.084264712458342
44	27.314287517985324	29.819265541032852	17.448385068824294	25.418061872157526
45	25.898819215563524	31.904037919778204	18.07975884536169	24.11738401929658
46	25.45399307153886	29.397455355616287	21.47686172801187	23.671689844832983
47	28.543629051137515	27.419292897143656	20.444852447977357	23.592225603741472
48	28.37202206375552	27.66357770582185	20.045163886777264	23.91923634364536
49	27.443071670379908	28.230191150824236	20.194044259030804	24.132692919765052
50	27.073632654779033	29.46973861762207	19.76418506408958	23.692443663509316
51	25.82017039700556	30.436856046358084	19.957019341296416	23.785954215339938
52	27.38457167958629	30.03324819620644	19.878160091116893	22.704020033090373
53	25.968892945542926	29.70615854444447	19.698109534919812	24.626838975092788
54	25.185561234286673	29.395166911731824	20.314937225616895	25.104334628364604
55	25.179485021214138	29.86774372584968	20.609278456273625	24.343492796662556
56	25.252583705753462	29.043325240483885	20.89015206315053	24.81393899061212
57	26.73802052234389	27.502729035092106	20.121839908883107	25.6374105336809
58	26.319472027061508	27.743489114109178	20.805584855236198	25.131454003593117
59	26.76540293709936	27.448648108351243	20.973614505051675	24.812334449497726
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	1.0
6	11.0
7	21.0
8	18.5
9	121.5
10	227.0
11	481.0
12	735.0
13	1292.5
14	2140.0
15	2430.0
16	2377.0
17	2324.0
18	2355.0
19	2639.5
20	2893.0
21	3718.5
22	4544.0
23	6239.0
24	7934.0
25	12197.5
26	25181.0
27	33901.0
28	44849.5
29	55798.0
30	64908.5
31	76705.0
32	79391.0
33	76966.0
34	74541.0
35	69238.0
36	64077.5
37	64220.0
38	64695.5
39	65171.0
40	69066.0
41	72961.0
42	76786.5
43	83514.5
44	86417.0
45	90734.0
46	95051.0
47	107316.0
48	124247.0
49	128913.0
50	153215.5
51	177518.0
52	186555.0
53	208737.5
54	221883.0
55	230483.5
56	239084.0
57	255111.5
58	282386.0
59	293633.0
60	287050.0
61	280467.0
62	253543.0
63	226619.0
64	213279.5
65	175477.5
66	151015.0
67	127047.0
68	103079.0
69	101220.5
70	88476.0
71	77590.0
72	67882.0
73	58174.0
74	48816.5
75	27748.0
76	16037.0
77	11805.0
78	7573.0
79	6137.0
80	4701.0
81	2938.0
82	1121.5
83	1068.0
84	621.0
85	174.0
86	128.0
87	45.5
88	9.0
89	5.5
90	2.0
91	1.5
92	0.5
93	0.0
94	0.5
95	1.0
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	3801710.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.212769036076764
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.281277873768	18.49907790439339
2	6.628756122519778	2.9448605749202317
3	2.817736994927217	1.8776922321818181
4	1.6558468505115491	1.4712377459811266
5	1.0528952935265692	1.1693859987138966
6	0.6953079350280946	0.926682874183832
7	0.48893332918744214	0.760239418069652
8	0.360467740842591	0.6405589337834279
9	0.25439104589714834	0.5085656592623423
>10	1.628117137709754	6.523064890694662
>50	0.1959574340739536	3.2284067675779013
>100	0.8393082091647694	45.52257769570997
>500	0.09172192099789124	13.350761530041295
>1k	0.009282111845325876	2.57688777448646
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.006444468410268011	0.0	0.0	0.0	0.0
12	0.009863982260614303	0.0	0.0	0.0	0.0
13	0.024015508810508956	0.0	0.0	0.0	0.0
14	0.15274705329969934	0.0	0.0	0.0	0.0
15	0.2377088205044572	0.0	0.0	0.0	0.0
16	0.3054678026467037	0.0	0.0	0.0	0.0
17	0.35704985388154276	0.0	0.0	0.0	0.0
18	0.4299644107520037	0.0	0.0	0.0	0.0
19	0.48854331340370516	0.0	0.0	0.0	0.0
20	0.536679546835503	0.0	0.0	0.0	0.0
21	0.5994670819183999	0.0	0.0	0.0	0.0
22	0.638107588427313	0.0	0.0	0.0	0.0
23	0.6800097850704026	0.0	0.0	0.0	5.260790538994295E-5
24	0.7405614841742268	0.0	0.0	0.0	5.260790538994295E-5
25	0.7847784286544739	0.0	0.0	0.0	5.260790538994295E-5
26	0.8377545893821464	0.0	0.0	0.0	5.260790538994295E-5
27	0.9154827695957871	0.0	0.0	0.0	5.260790538994295E-5
28	0.9876345118380939	0.0	0.0	0.0	5.260790538994295E-5
29	1.041820654389735	0.0	0.0	0.0	5.260790538994295E-5
30	1.10200409815583	0.0	0.0	0.0	5.260790538994295E-5
31	1.161819286584195	0.0	0.0	0.0	5.260790538994295E-5
32	1.2938388251602568	0.0	0.0	0.0	5.260790538994295E-5
33	1.3821411943572761	0.0	0.0	0.0	5.260790538994295E-5
34	1.4720218007159935	0.0	0.0	0.0	5.260790538994295E-5
35	1.539964910527105	0.0	0.0	0.0	5.260790538994295E-5
36	1.6069347740885023	0.0	0.0	0.0	5.260790538994295E-5
37	1.6755091787642928	0.0	0.0	0.0	5.260790538994295E-5
38	1.7427683858053349	0.0	0.0	0.0	5.260790538994295E-5
39	1.8134734106494184	0.0	0.0	0.0	5.260790538994295E-5
40	1.8955417430577293	0.0	0.0	0.0	5.260790538994295E-5
41	1.9838967201601385	0.0	0.0	0.0	5.260790538994295E-5
42	2.0815106886111776	0.0	0.0	0.0	5.260790538994295E-5
43	2.1811763653724245	0.0	0.0	0.0	5.260790538994295E-5
44	2.2871023828750747	0.0	0.0	0.0	5.260790538994295E-5
45	2.4007091545646566	0.0	0.0	0.0	5.260790538994295E-5
46	2.51252725747098	0.0	0.0	0.0	5.260790538994295E-5
47	2.6150074571705892	0.0	0.0	0.0	5.260790538994295E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATTA	20	3.1391668E-4	53.000004	47
CGTTAGT	20	3.1391668E-4	53.000004	18
TACTAAC	20	3.1391668E-4	53.000004	50
CACGTAA	20	3.1391668E-4	53.000004	50
CGTTTAG	20	3.1391668E-4	53.000004	15
ACTATCG	15	0.0066974503	53.0	36
GCGAATA	15	0.0066974503	53.0	36
TTCGTTA	30	7.0148053E-7	53.0	19
TACGGGT	25	1.4793839E-5	53.0	37
CTTATAC	30	7.0148053E-7	53.0	46
TACGATT	30	7.0148053E-7	53.0	29
TATTCGC	25	1.4793839E-5	53.0	15
CGATCAA	145	0.0	53.0	41
GCGTATA	105	0.0	53.0	24
GCGTAAT	30	7.0148053E-7	53.0	14
TACGTAA	35	3.3447577E-8	53.0	18
ACGGTTA	25	1.4793839E-5	53.0	26
CAATGCG	270	0.0	51.037033	44
CGATACA	235	0.0	50.74468	50
AACGATA	230	0.0	50.695652	48
>>END_MODULE