FastQCFastQC Report
Fri 17 Jun 2016
SRR1527918_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527918_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4246892
Sequences flagged as poor quality0
Sequence length52
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT351.0204349E-746.00000416
ACGTCGT351.0204349E-746.00000433
TTACGAC351.0204349E-746.00000443
GCGTATA453.110472E-1046.00000444
GTAACGA453.110472E-1046.00000433
GCGTACT1100.046.00000419
TGCGTAA351.0204349E-746.00000425
TAATACG206.313385E-446.039
ACGTTAC301.8625979E-646.045
ATTACGA405.6170393E-946.020
ACCGAAC501.6370905E-1146.045
TTCCGTA253.418723E-546.035
ATCGTTC301.8625979E-646.037
TACGATA206.313385E-446.027
TATTACG253.418723E-546.017
CTATTCG253.418723E-546.045
CGTAAAT253.418723E-546.024
ACCGTAC206.313385E-446.028
TCGACTA253.418723E-546.019
CATTTCG301.8625979E-646.021
Produced by FastQC (version 0.11.5)