##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1527918_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4246892 Sequences flagged as poor quality 0 Sequence length 52 %GC 57 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.90993389989668 31.0 31.0 34.0 30.0 34.0 2 32.40608708674485 34.0 31.0 34.0 31.0 34.0 3 32.56204396061873 34.0 31.0 34.0 31.0 34.0 4 36.09101408747856 37.0 35.0 37.0 35.0 37.0 5 35.91723500385694 37.0 35.0 37.0 35.0 37.0 6 36.04065631996293 37.0 35.0 37.0 35.0 37.0 7 35.47239910974896 37.0 35.0 37.0 33.0 37.0 8 35.873324774917755 37.0 35.0 37.0 35.0 37.0 9 37.76525751066898 39.0 38.0 39.0 35.0 39.0 10 37.45891065748788 39.0 38.0 39.0 34.0 39.0 11 38.09882827253436 39.0 38.0 39.0 37.0 39.0 12 38.12846288532885 39.0 39.0 39.0 37.0 39.0 13 37.97216788182982 39.0 38.0 39.0 35.0 39.0 14 35.70843054167612 35.0 35.0 40.0 33.0 40.0 15 34.72861447854101 35.0 33.0 39.0 26.0 40.0 16 34.614276981849315 35.0 33.0 40.0 25.0 40.0 17 34.95254129372727 35.0 33.0 40.0 29.0 40.0 18 35.207893678482996 35.0 34.0 40.0 30.0 40.0 19 35.3521813128283 35.0 34.0 40.0 31.0 40.0 20 35.36949091241313 35.0 34.0 40.0 31.0 40.0 21 35.07863256235383 35.0 34.0 40.0 30.0 40.0 22 35.10873975603806 35.0 34.0 40.0 30.0 40.0 23 35.03798095171716 35.0 34.0 40.0 30.0 40.0 24 35.00388613602606 35.0 34.0 40.0 30.0 40.0 25 35.17521448626431 35.0 34.0 40.0 30.0 40.0 26 35.25804682577282 35.0 34.0 40.0 30.0 40.0 27 35.296147158910564 35.0 34.0 40.0 31.0 40.0 28 35.34779645915177 35.0 34.0 40.0 31.0 40.0 29 35.39842242279766 35.0 35.0 40.0 31.0 40.0 30 35.382954876177685 35.0 35.0 40.0 31.0 40.0 31 35.381844887979256 35.0 35.0 40.0 31.0 40.0 32 35.33470665135822 35.0 35.0 40.0 31.0 40.0 33 35.30274798605663 35.0 35.0 40.0 31.0 40.0 34 35.21790005491074 35.0 34.0 40.0 31.0 40.0 35 35.15281222126675 35.0 34.0 40.0 31.0 40.0 36 35.12686830745872 35.0 34.0 40.0 31.0 40.0 37 35.01958985535775 35.0 34.0 40.0 31.0 40.0 38 34.78327704118683 35.0 34.0 39.0 30.0 40.0 39 34.78587117355468 35.0 34.0 39.0 30.0 40.0 40 34.73213210978758 35.0 34.0 39.0 30.0 40.0 41 34.67682837237208 35.0 34.0 39.0 30.0 40.0 42 34.603442705865845 35.0 34.0 39.0 30.0 40.0 43 34.49951493939568 35.0 34.0 38.0 30.0 40.0 44 34.43137310767498 35.0 34.0 38.0 29.0 40.0 45 34.36629728281294 35.0 34.0 38.0 29.0 40.0 46 34.27414565757735 35.0 34.0 38.0 29.0 40.0 47 34.18420411915349 35.0 34.0 37.0 29.0 40.0 48 34.11266121201104 35.0 34.0 37.0 29.0 40.0 49 34.0338496952595 35.0 34.0 37.0 29.0 40.0 50 33.92895769423852 35.0 33.0 36.0 28.0 40.0 51 33.83364752388335 35.0 33.0 36.0 28.0 40.0 52 33.22097124202829 35.0 33.0 35.0 27.0 39.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 4.0 12 4.0 13 15.0 14 19.0 15 64.0 16 219.0 17 611.0 18 1449.0 19 2848.0 20 5005.0 21 8201.0 22 12573.0 23 18188.0 24 25165.0 25 34136.0 26 44114.0 27 55500.0 28 69345.0 29 86673.0 30 108863.0 31 136491.0 32 174288.0 33 244699.0 34 582327.0 35 1095189.0 36 181640.0 37 353727.0 38 676112.0 39 329423.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 28.82710462145023 17.288548896463578 34.36633189636091 19.51801458572528 2 21.795444762899553 20.212192822421667 33.55894145648159 24.433420958197193 3 18.83965968524747 22.33423877979473 33.73290396836086 25.093197566596935 4 16.018890991341433 22.40104057272942 31.45667466938175 30.123393766547395 5 0.0 100.0 0.0 0.0 6 100.0 0.0 0.0 0.0 7 0.0 100.0 0.0 0.0 8 0.0 0.0 100.0 0.0 9 100.0 0.0 0.0 0.0 10 100.0 0.0 0.0 0.0 11 98.85801192966527 0.81709165196572 0.19868647472080758 0.12620994364820204 12 98.26162285266497 1.0128347977768213 0.3938645013812454 0.3316778481769727 13 92.02162428430015 3.802310018714863 2.3177420099216084 1.8583236870633866 14 57.49736513196003 23.545194933141694 6.796334825561846 12.161105109336427 15 36.52713560881699 20.097191075261627 19.124503283813198 24.251170032108185 16 31.76165063768987 15.636564339286235 26.984769097024362 25.61701592599953 17 24.174572840561993 15.966758749692717 27.89736588545223 31.96130252429306 18 24.144362512632767 17.452456996787298 32.21683056691811 26.186349923661822 19 29.45521101078153 18.574053684435583 27.907067097538622 24.063668207244262 20 33.51274296591484 18.90057482036275 24.11087920295595 23.475803010766462 21 32.809428636282725 19.793981104299334 21.906561315898777 25.490028943519167 22 31.487991688981026 20.893443958546627 22.202919217159277 25.41564513531307 23 30.306021438736845 23.179468656137242 21.930037307282596 24.58447259784332 24 33.057327570373815 22.13717702263208 21.58157071100466 23.223924695989442 25 32.39197041036127 21.577073304430627 22.97329435267014 23.05766193253796 26 31.638925595470756 18.904624840942503 24.570179792657783 24.88626977092895 27 33.59539164169939 20.060623156887438 22.42670640082206 23.917278800591117 28 34.40855100624174 19.712109467346945 20.046141978651683 25.833197547759635 29 29.98649365229914 20.818706950871366 19.11515527119597 30.07964412563352 30 28.558649478253745 19.943596399437517 22.330871611522028 29.166882510786714 31 26.378490434887443 19.709330964856182 21.71298446016522 32.19919414009115 32 26.590598489436513 23.610018809049066 20.28539929906388 29.513983402450545 33 30.499056721951018 22.29658771638177 20.383824217804456 26.820531343862758 34 29.80965845140399 20.942491591497973 22.32493786044006 26.92291209665798 35 32.148003763693545 20.19271975835505 20.881552909751413 26.777723568199992 36 35.13103229373387 19.552110107815317 20.548320983910116 24.768536614540704 37 32.76344206539748 20.983910115915354 22.15333001168855 24.099317806998624 38 28.186047584916217 22.637425204125748 27.903747022528474 21.272780188429564 39 28.583538267514218 25.414161697542582 25.23351194238045 20.768788092562747 40 28.542731955510053 27.793383961730132 21.25366032383211 22.410223758927707 41 30.8410479946276 26.906547187920015 19.486579832969618 22.76582498448277 42 30.593572899899502 26.292568777355296 19.996670506337342 23.117187816407856 43 30.835750002590128 24.57171032369083 20.13161625018955 24.46092342352949 44 28.770263053545982 22.541449135038047 19.650205373718006 29.03808243769797 45 26.15703436772115 24.66467242397499 19.78851828584292 29.389774922460944 46 25.929644549472886 22.644018260883488 20.862998164304624 30.563339025339005 47 27.364364339851356 19.8830344637914 22.994486320820027 29.758114875537217 48 27.812574466221417 19.98463817775446 22.300496457173857 29.902290898850264 49 28.28489634301979 21.932439063672916 21.93910276032449 27.843561832982804 50 27.790110979982536 23.185355313956656 21.64387509736532 27.38065860869549 51 26.344182993115904 21.787886294259426 23.853514523091242 28.01441618953343 52 27.956256010277635 20.375041324337893 25.62445666148327 26.044246003901208 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 3.0 8 6.0 9 62.0 10 118.0 11 186.5 12 255.0 13 485.5 14 792.5 15 869.0 16 1108.0 17 1347.0 18 1567.0 19 1787.0 20 2131.5 21 2476.0 22 3380.0 23 4284.0 24 6033.5 25 7783.0 26 9129.5 27 10476.0 28 13044.0 29 15612.0 30 19666.5 31 23721.0 32 23676.5 33 23632.0 34 27945.5 35 32259.0 36 36952.5 37 41646.0 38 45229.0 39 54845.5 40 60879.0 41 73271.5 42 85664.0 43 92572.0 44 99480.0 45 113535.0 46 127590.0 47 138484.5 48 149379.0 49 161959.0 50 174539.0 51 193654.5 52 212770.0 53 237366.5 54 261963.0 55 299295.5 56 336628.0 57 367159.0 58 397690.0 59 425867.5 60 454045.0 61 444972.5 62 435900.0 63 404293.0 64 335237.0 65 297788.0 66 248005.0 67 198222.0 68 159587.5 69 120953.0 70 108497.0 71 96041.0 72 82208.0 73 68375.0 74 57181.0 75 45987.0 76 31664.5 77 17342.0 78 14337.5 79 11333.0 80 7386.0 81 3439.0 82 2658.0 83 1877.0 84 1021.5 85 166.0 86 161.5 87 157.0 88 126.0 89 77.5 90 60.0 91 52.5 92 45.0 93 22.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 52 4246892.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 6.729257393840913 #Duplication Level Percentage of deduplicated Percentage of total 1 71.13441248310785 4.7868177115848285 2 8.625283100553762 1.1608350015674498 3 4.336832478143052 0.8755098605818064 4 2.5874407361414464 0.6964621881922002 5 1.6666396353999993 0.5607623544691884 6 1.1229027361257176 0.45337809237829063 7 0.7509063628204916 0.35371295358643867 8 0.5059487641106337 0.27237275694369234 9 0.3658770558316733 0.22158727948728293 >10 2.8711608087036957 4.261960918463557 >50 1.400292276681311 7.099212841662003 >100 4.298783340866465 63.846806601669094 >500 0.3095715678043841 13.317085391822165 >1k 0.023948653709554173 2.0934960475919997 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.004685779624252277 0.0 0.0 0.0 0.0 12 0.011938142057768364 0.0 0.0 0.0 0.0 13 0.020108823111112787 0.0 0.0 0.0 0.0 14 0.03449581482175671 0.0 0.0 0.0 0.0 15 0.04612785067291563 0.0 0.0 0.0 0.0 16 0.05272090743065753 0.0 0.0 0.0 0.0 17 0.0605854822773925 0.0 0.0 0.0 0.0 18 0.07052216067656064 0.0 0.0 0.0 0.0 19 0.08175390379599952 0.0 0.0 0.0 0.0 20 0.0931269267031043 0.0 0.0 0.0 0.0 21 0.10511216202342796 0.0 0.0 0.0 0.0 22 0.1187456615331871 0.0 0.0 0.0 0.0 23 0.13489865058965475 0.0 0.0 0.0 0.0 24 0.1509574531210118 0.0 0.0 0.0 0.0 25 0.1662392168202064 0.0 0.0 0.0 0.0 26 0.18286313850222705 0.0 0.0 0.0 0.0 27 0.20113532437368314 0.0 0.0 0.0 0.0 28 0.2167702875420425 0.0 0.0 0.0 0.0 29 0.234547994156668 0.0 0.0 0.0 0.0 30 0.25399751159200656 0.0 0.0 0.0 0.0 31 0.27040951359252835 0.0 0.0 0.0 0.0 32 0.2857383705542783 0.0 0.0 0.0 0.0 33 0.2973704064054372 0.0 0.0 0.0 0.0 34 0.3080841236367678 0.0 0.0 0.0 0.0 35 0.318350454873823 0.0 0.0 0.0 0.0 36 0.32885225242365473 0.0 0.0 0.0 0.0 37 0.34029591522459246 0.0 0.0 0.0 0.0 38 0.35536575924228825 0.0 0.0 0.0 0.0 39 0.37585132845384345 0.0 0.0 0.0 0.0 40 0.41072388937604254 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTACGT 35 1.0204349E-7 46.000004 16 ACGTCGT 35 1.0204349E-7 46.000004 33 TTACGAC 35 1.0204349E-7 46.000004 43 GCGTATA 45 3.110472E-10 46.000004 44 GTAACGA 45 3.110472E-10 46.000004 33 GCGTACT 110 0.0 46.000004 19 TGCGTAA 35 1.0204349E-7 46.000004 25 TAATACG 20 6.313385E-4 46.0 39 ACGTTAC 30 1.8625979E-6 46.0 45 ATTACGA 40 5.6170393E-9 46.0 20 ACCGAAC 50 1.6370905E-11 46.0 45 TTCCGTA 25 3.418723E-5 46.0 35 ATCGTTC 30 1.8625979E-6 46.0 37 TACGATA 20 6.313385E-4 46.0 27 TATTACG 25 3.418723E-5 46.0 17 CTATTCG 25 3.418723E-5 46.0 45 CGTAAAT 25 3.418723E-5 46.0 24 ACCGTAC 20 6.313385E-4 46.0 28 TCGACTA 25 3.418723E-5 46.0 19 CATTTCG 30 1.8625979E-6 46.0 21 >>END_MODULE