FastQCFastQC Report
Fri 17 Jun 2016
SRR1527917_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527917_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4210250
Sequences flagged as poor quality0
Sequence length52
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGCG206.3133787E-446.00000414
ACGTTAC206.3133787E-446.00000445
ACGATAA206.3133787E-446.00000428
TACGATA206.3133787E-446.00000427
TATTACG206.3133787E-446.00000417
CGTTTAG351.0204349E-746.00000432
TCGAACT206.3133787E-446.00000442
CTTAACG1750.046.042
TCGCATA501.6370905E-1146.025
GATACGC501.6370905E-1146.013
GTCGAAC253.418718E-546.041
ATTACGA650.046.020
CACGCTA301.8625924E-646.036
ACGATTC301.8625924E-646.035
ACGATAC253.418718E-546.023
CGAATAC253.418718E-546.034
CGTAAAT253.418718E-546.024
TTACGCG253.418718E-546.038
TTACGAC301.8625924E-646.043
CACGTTA301.8625924E-646.032
Produced by FastQC (version 0.11.5)