FastQCFastQC Report
Fri 17 Jun 2016
SRR1527916_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527916_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4267071
Sequences flagged as poor quality0
Sequence length59
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG150.006697505753.039
CAAACCG1650.053.035
ATTAGCG251.479415E-553.014
CGTATAC600.053.016
CGTTAGT150.006697505753.042
TTGCGAT203.139211E-453.049
CAATGCG203.139211E-453.019
ATACGCA150.006697505753.027
TCGACTA251.479415E-553.019
CGCTTAT150.006697505753.017
ACGGTAC307.0150054E-753.038
ATGGGGG3914800.051.9609347
GATGGGG4231250.051.9472056
TAGATGG1366750.051.8773774
TGGGGGG2309000.051.807568
TTAGATG451100.051.701733
CAGATGG1315550.051.662464
AGATGGG4330850.051.6116265
ATAGATG318400.051.5018843
GTTAGAT102000.051.4931372
Produced by FastQC (version 0.11.5)