##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527916_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4267071
Sequences flagged as poor quality	0
Sequence length	59
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.45602498763203	31.0	31.0	33.0	30.0	34.0
2	31.954166921525328	31.0	31.0	34.0	30.0	34.0
3	32.22218847542026	34.0	31.0	34.0	30.0	34.0
4	35.90144738627504	37.0	35.0	37.0	35.0	37.0
5	35.562573015541574	37.0	35.0	37.0	33.0	37.0
6	35.7672773666058	37.0	35.0	37.0	33.0	37.0
7	35.03510534509503	37.0	35.0	37.0	32.0	37.0
8	35.51326354775911	37.0	35.0	37.0	33.0	37.0
9	37.45497649324326	39.0	37.0	39.0	34.0	39.0
10	37.108064759175555	39.0	37.0	39.0	33.0	39.0
11	37.78583552980487	39.0	37.0	39.0	35.0	39.0
12	37.83380965538188	39.0	37.0	39.0	35.0	39.0
13	37.60981478864542	39.0	37.0	39.0	35.0	39.0
14	35.12789288015128	35.0	33.0	39.0	31.0	40.0
15	34.043348938885714	35.0	33.0	39.0	23.0	40.0
16	33.951212435884	35.0	33.0	39.0	23.0	40.0
17	34.240337927351106	35.0	33.0	39.0	25.0	40.0
18	34.48846292925522	35.0	33.0	40.0	27.0	40.0
19	34.68542824808868	35.0	33.0	40.0	28.0	40.0
20	34.78693792533567	35.0	34.0	40.0	29.0	40.0
21	34.682940827560635	35.0	34.0	40.0	29.0	40.0
22	34.64886452557269	35.0	34.0	40.0	28.0	40.0
23	34.592578609542706	35.0	33.0	40.0	28.0	40.0
24	34.5489728199976	35.0	33.0	39.0	27.0	40.0
25	34.50729669133698	35.0	33.0	39.0	27.0	40.0
26	34.43428900058143	35.0	33.0	39.0	27.0	40.0
27	34.379907435334445	35.0	33.0	39.0	27.0	40.0
28	34.451640012551934	35.0	33.0	39.0	27.0	40.0
29	34.46085382689906	35.0	33.0	39.0	27.0	40.0
30	34.447237226659695	35.0	33.0	39.0	27.0	40.0
31	34.482307418836015	35.0	34.0	39.0	28.0	40.0
32	34.37208005210131	35.0	33.0	39.0	27.0	40.0
33	34.27548545594859	35.0	33.0	39.0	27.0	40.0
34	34.193608683802076	35.0	33.0	39.0	27.0	40.0
35	34.08514974323136	35.0	33.0	39.0	27.0	40.0
36	33.962828835048676	35.0	33.0	39.0	26.0	40.0
37	33.825056109917085	35.0	33.0	38.0	25.0	40.0
38	33.52307543042991	35.0	33.0	38.0	23.0	40.0
39	33.532695378164554	35.0	33.0	38.0	24.0	40.0
40	33.47214025733343	35.0	33.0	38.0	23.0	40.0
41	33.45651900331632	35.0	33.0	38.0	24.0	40.0
42	33.42154067743424	35.0	33.0	38.0	24.0	40.0
43	33.27488270056908	35.0	33.0	37.0	23.0	40.0
44	33.20921798582681	35.0	33.0	37.0	23.0	40.0
45	33.11720311192385	35.0	33.0	37.0	23.0	40.0
46	32.97741284361099	35.0	32.0	37.0	23.0	40.0
47	32.920468161884344	35.0	32.0	36.0	23.0	40.0
48	32.822266608640916	35.0	32.0	36.0	23.0	40.0
49	32.763977210597155	35.0	32.0	36.0	24.0	40.0
50	32.61940403616438	35.0	32.0	36.0	23.0	39.0
51	32.51732277245914	35.0	32.0	35.0	23.0	39.0
52	32.413913197132175	35.0	32.0	35.0	23.0	39.0
53	32.25443213857937	35.0	31.0	35.0	23.0	39.0
54	32.160156228944864	34.0	31.0	35.0	23.0	39.0
55	32.07776083407096	34.0	31.0	35.0	23.0	38.0
56	31.92322719729763	34.0	31.0	35.0	23.0	38.0
57	31.82400949035064	34.0	31.0	35.0	23.0	38.0
58	31.67681179900686	34.0	31.0	35.0	22.0	37.0
59	31.19279290173517	34.0	30.0	35.0	22.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	7.0
13	19.0
14	88.0
15	396.0
16	1160.0
17	2811.0
18	5541.0
19	9465.0
20	14547.0
21	20762.0
22	28395.0
23	37432.0
24	48557.0
25	61379.0
26	75847.0
27	90586.0
28	106391.0
29	125663.0
30	153007.0
31	188421.0
32	240440.0
33	343336.0
34	728011.0
35	700643.0
36	257529.0
37	390771.0
38	526719.0
39	109144.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.887403092191345	17.501419592033972	33.823411890732544	19.787765425042142
2	21.44299919077981	20.6995618305859	33.31556939174436	24.54186958688993
3	18.863712368507578	22.484955136673378	33.57602908411882	25.075303410700222
4	16.045760663462126	22.627582245526266	31.23814907227932	30.088508018732284
5	0.0	100.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	0.0	100.0	0.0	0.0
8	0.0	0.0	100.0	0.0
9	100.0	0.0	0.0	0.0
10	100.0	0.0	0.0	0.0
11	98.83711801373823	0.8295620110375478	0.2047071632977281	0.1286128119264948
12	98.24050267736347	1.033355198448772	0.386705541107706	0.33943658308005653
13	91.96734715686709	3.815989937828548	2.3239594560296744	1.8927034492746897
14	57.33396514845898	23.74399207325118	6.799324407772919	12.12271837051692
15	36.576072908090815	20.221482136106943	19.08205886426544	24.120386091536794
16	31.8792211331848	15.674194312679587	26.878741881726363	25.567842672409245
17	24.180802241162617	15.98914102905717	27.805185336733324	32.024871393046894
18	24.102669020506102	17.556023792432796	32.12201062508686	26.219296561974243
19	29.502579169645877	18.672386749599433	27.924119378374535	23.900914702380156
20	33.6418353479471	18.99396564997395	24.031097678009107	23.333101324069837
21	32.57447555946456	20.021602640312288	21.859350360001038	25.544571440222107
22	31.478454424592417	21.053270498662897	22.161806072596402	25.30646900414828
23	30.286512692195654	23.306642893919506	21.987822560252688	24.419021853632152
24	33.14425750122273	22.21568377934185	21.538896353025294	23.101162366410122
25	32.358331979945966	21.69729540474016	23.01555329170759	22.928819323606287
26	31.602028651503574	18.953985063759195	24.686254341678403	24.757731943058833
27	33.628945944419485	20.053498055223358	22.512585330780762	23.80497066957639
28	34.404981777898705	19.75755735022923	20.08040175567737	25.757059116194693
29	29.97011298851132	20.856531330273153	19.119602181449523	30.053753499766
30	28.442390576580518	20.019423159352165	22.379754168608866	29.15843209545845
31	26.262136252244222	19.784554791799806	21.720308848856746	32.23300010709922
32	26.448938862278133	23.736774007275717	20.251455858128445	29.56283127231771
33	30.45053621090439	22.44272476366107	20.371374181493582	26.73536484394096
34	29.825470445652297	20.96813012954319	22.33194619916097	26.874453225643542
35	32.0907479627126	20.256096043398387	20.93703151412292	26.716124479766094
36	35.09001842247294	19.628077432974518	20.589603500855738	24.692300643696814
37	32.669997757243785	21.077338530340835	22.218214789489092	24.034448922926288
38	28.004807044457426	22.786520308661373	27.991402064788705	21.217270582092493
39	28.393410843175566	25.569553447786546	25.346824554829293	20.69021115420859
40	28.380287086856537	28.090533295555666	21.25851667338087	22.27066294420693
41	30.744086517426123	27.10664997137381	19.43928282421361	22.70998068698646
42	30.484048660076198	26.56222500164633	19.919190470465573	23.0345358678119
43	30.681045616536494	24.77992515240548	20.126288032235696	24.41274119882233
44	28.695515026583806	22.599670828069186	19.61734407512788	29.08747007021913
45	25.961485056142724	24.768582477301173	19.82537904806365	29.444553418492454
46	25.8000862886978	22.785606332774872	20.832955439457184	30.58135193907015
47	27.263924129689897	19.999784395431902	22.950965662394648	29.785325812483553
48	27.754588569067636	20.041499192303107	22.293840435277502	29.910071803351762
49	28.239816023684632	22.116927513041144	21.835540116393656	27.807716346880568
50	27.682056380125854	23.340085037253893	21.59462544682289	27.383233135797365
51	26.2216635251675	21.875895667074673	23.84602927863164	28.056411529126184
52	27.896629795941998	20.48086380564092	25.663130517397065	25.959375881020026
53	29.159322636065816	21.11049945032553	23.808392220330994	25.92178569327766
54	27.796959553754792	19.926994418419568	24.02261410696002	28.253431920865623
55	27.345267983588744	20.512220209131744	24.51189586486843	27.63061594241108
56	25.761207160602673	22.38711284625918	23.962268263171623	27.88941172996653
57	27.442922791769814	20.911721412650504	25.831278645234633	25.81407715034505
58	26.37160244111242	21.199366028828674	24.687519846752025	27.741511683306886
59	26.321825908216667	20.770594161662647	22.21029366513939	30.697286264981294
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	3.5
7	7.0
8	39.0
9	109.5
10	148.0
11	281.0
12	414.0
13	459.5
14	619.5
15	734.0
16	821.0
17	908.0
18	998.5
19	1196.5
20	1304.0
21	1734.0
22	2164.0
23	3102.0
24	4040.0
25	5253.5
26	7955.5
27	9444.0
28	12085.0
29	14726.0
30	15961.5
31	21623.0
32	26049.0
33	29734.5
34	33420.0
35	34691.5
36	39231.5
37	42500.0
38	45774.5
39	49049.0
40	57003.5
41	64958.0
42	69193.0
43	85937.0
44	98446.0
45	102115.0
46	105784.0
47	116534.0
48	138110.5
49	148937.0
50	158102.5
51	167268.0
52	181035.0
53	225290.0
54	255778.0
55	292121.5
56	328465.0
57	350188.0
58	396397.5
59	420884.0
60	399715.0
61	378546.0
62	350238.0
63	321930.0
64	298294.5
65	243262.5
66	211866.0
67	181632.0
68	151398.0
69	126340.5
70	91347.0
71	81411.0
72	71841.0
73	62271.0
74	51896.0
75	30889.0
76	20257.0
77	14989.0
78	9721.0
79	6905.5
80	4090.0
81	3187.5
82	1628.5
83	972.0
84	682.5
85	393.0
86	277.0
87	123.5
88	86.0
89	73.0
90	60.0
91	35.5
92	8.0
93	5.0
94	3.0
95	1.0
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	4267071.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.472504076557122
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.14942612740441	8.184201836998424
2	7.7349037232917865	1.6200762154790023
3	3.391409757317817	1.0654965752635932
4	1.9348109371253654	0.8104926170565078
5	1.21494068378662	0.6361735631865237
6	0.761618068711785	0.4785628997618238
7	0.4939726335421714	0.36211912929346335
8	0.34262241856845577	0.2870491740142411
9	0.25673607783546126	0.24198026595580394
>10	1.8903462401688307	4.409717984955926
>50	0.9502274246749414	7.517775142544275
>100	2.69546129333895	61.20801317122202
>500	0.17131977535579906	11.452599193267158
>1k	0.012204838877589906	1.7257422310012471
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.004429267757672652	0.0	0.0	0.0	0.0
12	0.011553592616574695	0.0	0.0	0.0	0.0
13	0.01909975250001699	0.0	0.0	0.0	0.0
14	0.03302030830984533	0.0	0.0	0.0	0.0
15	0.042628772757706636	0.0	0.0	0.0	0.0
16	0.049401568429491796	0.0	0.0	0.0	0.0
17	0.05659619912581722	0.0	0.0	0.0	0.0
18	0.0658062638282794	0.0	0.0	0.0	0.0
19	0.07548503411356408	0.0	0.0	0.0	0.0
20	0.08542159246940115	0.0	0.0	0.0	0.0
21	0.09631899727002434	0.0	0.0	0.0	2.343527914112514E-5
22	0.10859908353997391	0.0	0.0	0.0	2.343527914112514E-5
23	0.12273055686207236	0.0	0.0	0.0	2.343527914112514E-5
24	0.13651050099705395	0.0	0.0	0.0	2.343527914112514E-5
25	0.1523996202547368	0.0	0.0	0.0	2.343527914112514E-5
26	0.16728102250935126	0.0	0.0	0.0	2.343527914112514E-5
27	0.1827014361842116	0.0	0.0	0.0	2.343527914112514E-5
28	0.19755940315968495	0.0	0.0	0.0	2.343527914112514E-5
29	0.21234706429773492	0.0	0.0	0.0	2.343527914112514E-5
30	0.22889237137136925	0.0	0.0	0.0	2.343527914112514E-5
31	0.24480492590819322	0.0	0.0	0.0	2.343527914112514E-5
32	0.2576474588775298	0.0	0.0	0.0	2.343527914112514E-5
33	0.2688729575861287	0.0	0.0	0.0	2.343527914112514E-5
34	0.2794422684787762	0.0	0.0	0.0	2.343527914112514E-5
35	0.2880430159235691	0.0	0.0	0.0	2.343527914112514E-5
36	0.2969484219971967	0.0	0.0	0.0	2.343527914112514E-5
37	0.307119333144445	0.0	0.0	0.0	2.343527914112514E-5
38	0.3203602658591807	0.0	0.0	0.0	2.343527914112514E-5
39	0.3407020881536773	0.0	0.0	0.0	2.343527914112514E-5
40	0.371355433270269	0.0	0.0	0.0	2.343527914112514E-5
41	0.3946969712948296	0.0	0.0	0.0	2.343527914112514E-5
42	0.4089690562917748	0.0	0.0	0.0	2.343527914112514E-5
43	0.42192876565681703	0.0	0.0	0.0	2.343527914112514E-5
44	0.43313082908627487	0.0	0.0	0.0	2.343527914112514E-5
45	0.4451765625648132	0.0	0.0	0.0	2.343527914112514E-5
46	0.4581362719298554	0.0	0.0	0.0	2.343527914112514E-5
47	0.47051009931636945	0.0	0.0	0.0	2.343527914112514E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	15	0.0066975057	53.0	39
CAAACCG	165	0.0	53.0	35
ATTAGCG	25	1.479415E-5	53.0	14
CGTATAC	60	0.0	53.0	16
CGTTAGT	15	0.0066975057	53.0	42
TTGCGAT	20	3.139211E-4	53.0	49
CAATGCG	20	3.139211E-4	53.0	19
ATACGCA	15	0.0066975057	53.0	27
TCGACTA	25	1.479415E-5	53.0	19
CGCTTAT	15	0.0066975057	53.0	17
ACGGTAC	30	7.0150054E-7	53.0	38
ATGGGGG	391480	0.0	51.960934	7
GATGGGG	423125	0.0	51.947205	6
TAGATGG	136675	0.0	51.877377	4
TGGGGGG	230900	0.0	51.80756	8
TTAGATG	45110	0.0	51.70173	3
CAGATGG	131555	0.0	51.66246	4
AGATGGG	433085	0.0	51.611626	5
ATAGATG	31840	0.0	51.501884	3
GTTAGAT	10200	0.0	51.493137	2
>>END_MODULE