FastQCFastQC Report
Fri 17 Jun 2016
SRR1527915_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527915_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2502111
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAA750.046.00000425
TAACCGT750.046.00000419
TTAACCG750.046.00000432
ACGCGAA253.418317E-546.042
TTTAGCG253.418317E-546.039
AACGTAC301.8622886E-646.040
ACTATAC301.8622886E-646.021
ACAGTCG301.8622886E-646.014
CGAACTG253.418317E-546.021
CGAACCT551.8189894E-1246.026
TTAATCG206.312886E-446.038
CGAAAGT405.6152203E-946.035
CGAAAAC253.418317E-546.030
ACCATAG800.046.038
CTATAGT501.6370905E-1146.034
TAGCGAT600.046.016
CCGGATA800.046.024
ACCGATA551.8189894E-1246.032
ATAGCCG206.312886E-446.027
CAACCGT206.312886E-446.046
Produced by FastQC (version 0.11.5)