##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527915_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2502111
Sequences flagged as poor quality	0
Sequence length	52
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.653313142382572	31.0	31.0	34.0	30.0	34.0
2	32.281429161216266	33.0	31.0	34.0	30.0	34.0
3	32.469921997865	34.0	31.0	34.0	31.0	34.0
4	36.01821581856281	37.0	35.0	37.0	35.0	37.0
5	36.05723207323736	37.0	35.0	37.0	35.0	37.0
6	36.12387659860014	37.0	36.0	37.0	35.0	37.0
7	36.05120795999858	37.0	35.0	37.0	35.0	37.0
8	35.072894048265645	35.0	35.0	37.0	33.0	37.0
9	37.82268932113723	39.0	37.0	39.0	35.0	39.0
10	37.52673282680105	39.0	37.0	39.0	35.0	39.0
11	38.09997558061973	39.0	38.0	39.0	37.0	39.0
12	38.145101076650874	39.0	39.0	39.0	37.0	39.0
13	38.08137808434558	39.0	39.0	39.0	37.0	39.0
14	38.596082667795315	40.0	39.0	40.0	36.0	40.0
15	36.91512886518624	39.0	35.0	40.0	33.0	40.0
16	36.61318582588862	39.0	35.0	40.0	31.0	40.0
17	36.617369493199945	39.0	35.0	40.0	31.0	40.0
18	36.82078253123063	39.0	35.0	40.0	31.0	40.0
19	37.03933038941918	39.0	35.0	40.0	32.0	40.0
20	37.12937155865587	39.0	35.0	40.0	33.0	40.0
21	36.95579732473899	39.0	35.0	40.0	32.0	40.0
22	36.987974953948886	39.0	35.0	40.0	32.0	40.0
23	36.93877529813825	39.0	35.0	40.0	32.0	40.0
24	36.89343478366867	39.0	35.0	40.0	32.0	40.0
25	36.96458270636275	39.0	35.0	40.0	32.0	40.0
26	36.95212562512215	39.0	35.0	40.0	32.0	40.0
27	36.83666791761037	39.0	35.0	40.0	32.0	40.0
28	36.73988084461481	39.0	35.0	40.0	32.0	40.0
29	36.730488775278154	39.0	35.0	40.0	32.0	40.0
30	36.64943082061507	39.0	35.0	40.0	32.0	40.0
31	36.54670596148612	39.0	35.0	40.0	32.0	40.0
32	36.44705250886152	39.0	35.0	40.0	31.0	40.0
33	36.31982354100198	38.0	35.0	40.0	31.0	40.0
34	36.21327111387145	38.0	35.0	40.0	31.0	40.0
35	36.04738278997215	38.0	35.0	40.0	31.0	40.0
36	35.89671441434852	38.0	35.0	40.0	31.0	40.0
37	35.71647740647797	37.0	35.0	40.0	30.0	40.0
38	35.46760515420779	37.0	35.0	40.0	30.0	40.0
39	35.22472624116196	37.0	35.0	40.0	29.0	40.0
40	34.97176264362373	36.0	34.0	40.0	28.0	40.0
41	34.69367945706645	36.0	34.0	40.0	27.0	40.0
42	34.42719487664616	35.0	34.0	40.0	26.0	40.0
43	34.17765358930919	35.0	34.0	39.0	25.0	40.0
44	33.926763840612985	35.0	33.0	39.0	24.0	40.0
45	33.7217545504576	35.0	33.0	39.0	23.0	40.0
46	33.54632828040003	35.0	33.0	39.0	23.0	40.0
47	33.375807468173875	35.0	33.0	38.0	23.0	40.0
48	33.19134762606455	35.0	33.0	38.0	23.0	40.0
49	33.04050379859247	35.0	33.0	37.0	23.0	40.0
50	32.84497370420417	35.0	33.0	37.0	22.0	40.0
51	32.66330710348182	35.0	33.0	37.0	22.0	40.0
52	32.040435056638174	35.0	32.0	36.0	20.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	4.0
14	12.0
15	15.0
16	94.0
17	277.0
18	729.0
19	1423.0
20	2654.0
21	4433.0
22	6814.0
23	10047.0
24	14684.0
25	20078.0
26	27105.0
27	34620.0
28	44054.0
29	55483.0
30	65513.0
31	74010.0
32	84475.0
33	107939.0
34	186766.0
35	325933.0
36	235781.0
37	433607.0
38	563317.0
39	202242.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.689889857004747	18.73665876533855	34.94900905675248	16.62444232090423
2	24.748302533340848	20.827733062202277	34.34515894778449	20.078805456672384
3	21.373312375030523	22.566025248280354	36.477838113496965	19.582824263192162
4	17.22237742450275	21.26104717176816	36.20790604413633	25.308669359592763
5	0.0	100.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	100.0	0.0	0.0	0.0
8	0.0	0.0	100.0	0.0
9	0.0	0.0	100.0	0.0
10	100.0	0.0	0.0	0.0
11	99.54686262919591	0.34311027768152574	0.04508193281593023	0.06494516030663706
12	99.42924194809902	0.3531817733106165	0.07305830956340466	0.14451796902695363
13	95.984550645435	2.1053022827524437	0.9154669796823562	0.9946800921302053
14	56.65416122626055	27.55089602339784	5.9419426236485915	9.85300012669302
15	33.33673046479553	28.651047055866023	17.37792607921871	20.63429640011974
16	30.02017096763493	21.021769218072258	25.85964411650802	23.09841569778479
17	22.68816211590933	21.490013832319992	28.24546952553264	27.576354526238045
18	22.75522548759827	22.320752356709995	28.967220079364985	25.956802076326753
19	25.444274854313022	21.828607923469423	26.276891792570357	26.450225429647205
20	27.465687973075536	22.0991394866175	24.794503521226677	25.640669019080285
21	30.107377330582057	22.830481941048976	20.751197688671684	26.310943039697282
22	30.22639683051631	23.353959916246723	20.80487236577434	25.61477088746263
23	27.839812062694264	23.453755648730212	20.049350328582545	28.65708195999298
24	28.746846163099875	25.312665984842397	19.397780514133867	26.54270733792386
25	31.039989832585363	21.496648230234392	22.73140560111042	24.73195633606982
26	31.788717606852774	21.774413685084316	20.877371147802794	25.559497560260112
27	30.912937115899336	23.753142846180687	20.11845197914881	25.215468058771172
28	30.34641548676298	24.27570159757101	20.034083220128924	25.34379969553709
29	31.018567921247296	23.941583726701175	19.01334513137107	26.026503220680457
30	30.733448675938035	24.623727724309592	18.3373159703946	26.30550762935777
31	29.881887733997413	24.47829053147522	19.835450945221854	25.80437078930551
32	30.44529199543905	24.73399461494714	18.904357160813408	25.916356228800403
33	28.725304353004322	26.35146881972862	18.740695356840682	26.182531470426373
34	28.642174547811827	26.05831635766759	18.842689233211477	26.45681986130911
35	30.275395456076886	26.22849266079722	18.902598645703566	24.59351323742232
36	30.83636177611625	25.863121180475208	18.988805852338288	24.31171119107026
37	31.380941932632084	26.381723272868392	18.801404094382704	23.43593070011682
38	31.069884589452666	27.014548914896263	19.292189675038397	22.623376820612677
39	30.707630476825372	27.210023855856114	20.528665594771773	21.55368007254674
40	31.7895169319027	26.341397324099532	19.302620866939957	22.56646487705781
41	30.930841997017716	26.660367985273236	17.78002654558491	24.628763472124138
42	31.659506712531936	25.71540591124854	18.924220388304118	23.700866987915404
43	32.233182300865145	25.804730485577977	18.222452960719966	23.739634252836904
44	30.131996542119833	26.563929417999443	19.98316621444852	23.320907825432204
45	30.34261869277582	25.325575084398732	19.567357323476056	24.76444889934939
46	29.149186426981053	24.32853698337124	20.53605935148361	25.986217238164095
47	29.517395511230315	23.12431382940245	21.737964462807604	25.620326196559624
48	29.080524405192254	23.668494323393325	21.335943928946396	25.915037342468022
49	29.45736619998074	22.42658299332044	21.626458618342674	26.489592188356152
50	29.158658428822704	23.367868172115465	21.809743852291124	25.663729546770707
51	28.085844313062054	24.596710537622034	22.158329506564655	25.15911564275126
52	29.8598663288719	24.152565573629627	21.013736001320485	24.973832096177986
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	2.5
8	4.0
9	58.0
10	112.0
11	390.5
12	669.0
13	1224.5
14	2102.5
15	2425.0
16	3053.5
17	3682.0
18	3982.5
19	4283.0
20	5014.0
21	5745.0
22	7298.5
23	8852.0
24	11189.5
25	13527.0
26	16555.5
27	19584.0
28	22864.0
29	26144.0
30	29505.0
31	32866.0
32	34417.0
33	35968.0
34	37425.5
35	38883.0
36	38760.5
37	38638.0
38	38411.5
39	39985.5
40	41786.0
41	45058.5
42	48331.0
43	48958.5
44	49586.0
45	56967.0
46	64348.0
47	69958.5
48	75569.0
49	90023.0
50	104477.0
51	111003.5
52	117530.0
53	131531.0
54	145532.0
55	156184.5
56	166837.0
57	184326.0
58	201815.0
59	208217.5
60	214620.0
61	217015.0
62	219410.0
63	201232.0
64	177817.5
65	172581.0
66	150944.5
67	129308.0
68	112656.5
69	96005.0
70	91192.0
71	86379.0
72	69723.5
73	53068.0
74	41751.0
75	30434.0
76	24180.0
77	17926.0
78	13641.0
79	9356.0
80	5264.0
81	1172.0
82	1177.5
83	1183.0
84	690.5
85	198.0
86	189.0
87	180.0
88	129.0
89	39.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2502111.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.389742625293335
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.75306205921159	10.812627171771833
2	6.538116606200023	1.7508739722234927
3	2.8766247989076366	1.1555179706072833
4	1.7970049748318964	0.9624573643748328
5	1.2089771767366995	0.8093946618179088
6	0.823829939588612	0.6618522514861482
7	0.6311032087519369	0.5915216674629645
8	0.4476409210612272	0.4795037377247266
9	0.36968869065286397	0.44550327773911813
>10	2.131817904868628	5.2679495216622945
>50	0.4254003301470449	4.2771162142057815
>100	1.8258512789054426	56.953987432642684
>500	0.15403457815115174	13.065791782700508
>1k	0.01684753198528222	2.765902973580418
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0014387850898701137	0.0	0.0	0.0	0.0
12	0.006914161681875824	0.0	0.0	0.0	0.0
13	0.029535060594833722	0.0	0.0	0.0	0.0
14	0.0808916950526975	0.0	0.0	0.0	0.0
15	0.14703584293422634	0.0	0.0	0.0	0.0
16	0.19039922689281172	0.0	0.0	0.0	0.0
17	0.2155779659655387	0.0	0.0	0.0	0.0
18	0.25062836940487454	0.0	0.0	0.0	0.0
19	0.2809227887971397	0.0	0.0	0.0	0.0
20	0.3129757232992461	0.0	0.0	0.0	0.0
21	0.3422310201266051	0.0	0.0	0.0	0.0
22	0.385034876550241	0.0	0.0	0.0	0.0
23	0.4255206903290861	0.0	0.0	0.0	0.0
24	0.47304056454729626	0.0	0.0	0.0	0.0
25	0.5232381776827647	0.0	0.0	0.0	0.0
26	0.5775922810778579	0.0	0.0	0.0	0.0
27	0.6373817948124604	0.0	0.0	0.0	0.0
28	0.6965718147596169	0.0	0.0	0.0	0.0
29	0.7576002823216076	0.0	0.0	0.0	0.0
30	0.8287002455126891	0.0	0.0	0.0	0.0
31	0.8928860470218947	0.0	0.0	0.0	0.0
32	0.9484391379918796	0.0	0.0	0.0	0.0
33	1.0013944225495992	0.0	0.0	0.0	0.0
34	1.0484347017378526	0.0	0.0	0.0	0.0
35	1.0924375457363802	0.0	0.0	0.0	0.0
36	1.1357209971899729	0.0	0.0	0.0	0.0
37	1.174688093373955	0.0	0.0	0.0	0.0
38	1.2157334346877497	0.0	0.0	0.0	0.0
39	1.2589369536363495	0.0	0.0	0.0	0.0
40	1.3068564903795234	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTAA	75	0.0	46.000004	25
TAACCGT	75	0.0	46.000004	19
TTAACCG	75	0.0	46.000004	32
ACGCGAA	25	3.418317E-5	46.0	42
TTTAGCG	25	3.418317E-5	46.0	39
AACGTAC	30	1.8622886E-6	46.0	40
ACTATAC	30	1.8622886E-6	46.0	21
ACAGTCG	30	1.8622886E-6	46.0	14
CGAACTG	25	3.418317E-5	46.0	21
CGAACCT	55	1.8189894E-12	46.0	26
TTAATCG	20	6.312886E-4	46.0	38
CGAAAGT	40	5.6152203E-9	46.0	35
CGAAAAC	25	3.418317E-5	46.0	30
ACCATAG	80	0.0	46.0	38
CTATAGT	50	1.6370905E-11	46.0	34
TAGCGAT	60	0.0	46.0	16
CCGGATA	80	0.0	46.0	24
ACCGATA	55	1.8189894E-12	46.0	32
ATAGCCG	20	6.312886E-4	46.0	27
CAACCGT	20	6.312886E-4	46.0	46
>>END_MODULE