FastQCFastQC Report
Fri 17 Jun 2016
SRR1527914_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527914_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2478909
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAAG405.6152203E-946.00000444
AACCGTA206.3128734E-446.00000418
AACGTAC206.3128734E-446.00000440
CAAACTA351.0202166E-746.00000433
AAATCGT206.3128734E-446.00000435
CGAACCT351.0202166E-746.00000426
TACCATA206.3128734E-446.00000436
CTATCGA351.0202166E-746.00000443
CGTGCAA206.3128734E-446.00000421
GACGTAA206.3128734E-446.00000443
CCGGATA351.0202166E-746.00000424
TAAACCC206.3128734E-446.00000429
GTGATAG206.3128734E-446.00000423
CGATTTC750.046.00000435
GTCTAAC206.3128734E-446.00000442
CAATTCA206.3128734E-446.00000426
TAGCCTA206.3128734E-446.00000438
TTATCGT206.3128734E-446.00000427
CGTTCGA351.0202166E-746.00000423
CGTTAAC206.3128734E-446.00000429
Produced by FastQC (version 0.11.5)