##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527914_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2478909
Sequences flagged as poor quality	0
Sequence length	52
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.617143267461614	31.0	31.0	34.0	30.0	34.0
2	32.131914886750586	33.0	31.0	34.0	30.0	34.0
3	32.40538115759796	34.0	31.0	34.0	30.0	34.0
4	35.98535000679735	37.0	35.0	37.0	35.0	37.0
5	36.04131817666562	37.0	35.0	37.0	35.0	37.0
6	36.10853443994919	37.0	35.0	37.0	35.0	37.0
7	36.05889647421507	37.0	35.0	37.0	35.0	37.0
8	35.03923742259195	35.0	35.0	37.0	33.0	37.0
9	37.8214218432383	39.0	37.0	39.0	35.0	39.0
10	37.52934819309624	39.0	37.0	39.0	35.0	39.0
11	38.09543149829219	39.0	38.0	39.0	37.0	39.0
12	38.13147719420116	39.0	39.0	39.0	37.0	39.0
13	38.09076573605566	39.0	39.0	39.0	37.0	39.0
14	38.62744174957612	40.0	39.0	40.0	36.0	40.0
15	36.94498265164231	39.0	35.0	40.0	33.0	40.0
16	36.6579914793161	39.0	35.0	40.0	31.0	40.0
17	36.68009515476365	39.0	35.0	40.0	31.0	40.0
18	36.8990390530673	39.0	35.0	40.0	32.0	40.0
19	37.10593652288164	39.0	35.0	40.0	33.0	40.0
20	37.17249806265579	39.0	35.0	40.0	33.0	40.0
21	37.188767719992946	39.0	35.0	40.0	33.0	40.0
22	37.123820600110776	39.0	35.0	40.0	33.0	40.0
23	37.04562369978083	39.0	35.0	40.0	32.0	40.0
24	37.00559923740646	39.0	35.0	40.0	32.0	40.0
25	36.93279019116878	39.0	35.0	40.0	32.0	40.0
26	36.9135615708362	39.0	35.0	40.0	32.0	40.0
27	36.80185476756105	39.0	35.0	40.0	32.0	40.0
28	36.552040433916694	39.0	35.0	40.0	31.0	40.0
29	36.64325515781338	39.0	35.0	40.0	32.0	40.0
30	36.634518249762294	39.0	35.0	40.0	32.0	40.0
31	36.57549631712983	39.0	35.0	40.0	32.0	40.0
32	36.47048278093306	39.0	35.0	40.0	31.0	40.0
33	36.342619273236735	38.0	35.0	40.0	31.0	40.0
34	36.25425580366201	38.0	35.0	40.0	31.0	40.0
35	36.02835521594379	38.0	35.0	40.0	31.0	40.0
36	35.515136295846276	38.0	35.0	40.0	29.0	40.0
37	35.54251809969628	37.0	35.0	40.0	30.0	40.0
38	35.40809848203383	37.0	35.0	40.0	29.0	40.0
39	35.205492012816926	37.0	34.0	40.0	29.0	40.0
40	34.96089045624507	36.0	34.0	40.0	28.0	40.0
41	34.71145975911177	36.0	34.0	40.0	27.0	40.0
42	34.30179929961124	35.0	34.0	40.0	26.0	40.0
43	34.059560072596454	35.0	33.0	39.0	24.0	40.0
44	33.80512677149504	35.0	33.0	39.0	23.0	40.0
45	33.37748864520642	35.0	33.0	39.0	23.0	40.0
46	33.34369918379416	35.0	33.0	39.0	23.0	40.0
47	33.24590091850891	35.0	33.0	38.0	23.0	40.0
48	33.060406412659766	35.0	33.0	38.0	22.0	40.0
49	32.92454785552838	35.0	33.0	37.0	22.0	40.0
50	32.67093225285801	35.0	33.0	37.0	20.0	40.0
51	32.572144842751385	35.0	33.0	37.0	20.0	40.0
52	32.07878022146033	35.0	32.0	36.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	7.0
13	10.0
14	6.0
15	25.0
16	75.0
17	263.0
18	692.0
19	1392.0
20	2510.0
21	4215.0
22	6765.0
23	10061.0
24	14543.0
25	19947.0
26	27008.0
27	35376.0
28	44440.0
29	55574.0
30	66275.0
31	75173.0
32	85168.0
33	110196.0
34	187404.0
35	321220.0
36	237535.0
37	429616.0
38	544192.0
39	199220.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.75542869867349	18.739937609650053	34.85960961051818	16.645024081158283
2	24.7735596587047	20.91839595564016	34.23086527177884	20.077179113876305
3	21.41256496305431	22.534671502665084	36.48621228128987	19.56655125299073
4	17.251177836701547	21.205135000921775	36.21294690527163	25.330740257105038
5	0.0	100.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	100.0	0.0	0.0	0.0
8	0.0	0.0	100.0	0.0
9	0.0	0.0	100.0	0.0
10	100.0	0.0	0.0	0.0
11	99.54484008892621	0.3425297177104928	0.0484487328901545	0.06418146047313555
12	99.43325874406847	0.35608406762813805	0.07321769375156571	0.13743949455183713
13	95.98315226577499	2.107338349249609	0.9101584608390223	0.9993509241363842
14	56.642055033081085	27.569789774453195	5.955926578991	9.832228613474719
15	33.44850496730618	28.654904234080398	17.354973498422087	20.541617300191334
16	30.052172145084793	21.13171560553453	25.768029403257643	23.048082846123034
17	22.705835510702492	21.58118753048216	28.19179727856085	27.521179680254498
18	22.728869837497058	22.393843420633836	28.994408427255703	25.882878314613407
19	25.414325414930524	21.87575259922813	26.29991661654381	26.41000536929754
20	27.46688159992965	22.186857202099795	24.776383481604206	25.569877716366353
21	29.787337897437943	23.002014192533892	20.707859788318167	26.50278812170999
22	30.28578297952849	23.323486259479473	20.818472965324663	25.57225779566737
23	27.83849669350509	23.491221339710332	20.02187252537306	28.648409441411527
24	28.68072204344734	25.376445847749956	19.416001152119744	26.52683095668296
25	31.019129786531092	21.569650196921305	22.67913021413856	24.732089802409043
26	31.76195657041061	21.878253699510548	20.869947222750007	25.48984250732883
27	30.938247430623715	23.77432975554972	20.08266539836678	25.204757415459785
28	30.40732838518881	24.325943388805317	19.989075839411612	25.277652386594262
29	31.053540085577968	23.99753278559237	19.015381363333628	25.93354576549603
30	30.74703427999979	24.673112244136433	18.30732794144521	26.272525534418566
31	29.84631545571056	24.557537206892224	19.784227658215773	25.811919679181443
32	30.396960921114896	24.797320111387712	18.918443557226183	25.88727541027121
33	28.68632934891922	26.419606367155872	18.764625889857193	26.129438394067712
34	28.533237807438677	26.124758916119955	18.878304931726014	26.463698344715358
35	30.25625386006505	26.37962910296425	18.834132273512257	24.52998476345844
36	30.82009061244281	25.78448825672907	19.03498676232165	24.360434368506468
37	31.309539801582066	26.44764289451529	18.80835480447245	23.434462499430193
38	31.024454709713023	27.03265831863937	19.314303187410268	22.628583784237343
39	30.64150398421241	27.276071852577083	20.459161671525656	21.62326249168485
40	31.782731838885574	26.39729816624975	19.27618157826689	22.543788416597785
41	30.95458526311373	26.672499877970512	17.787220103682706	24.58569475523305
42	31.723552578977284	25.731763449162514	18.969716113015846	23.574967858844353
43	32.15382250820824	25.86319223497111	18.239354490221302	23.74363076659934
44	30.272188289283715	26.59766857113351	19.912550238834907	23.217592900747867
45	30.384616781011324	25.385199698738436	19.580266964216918	24.649916556033318
46	29.252707541906542	24.401460481203628	20.48413233402275	25.861699642867087
47	29.481558217748212	23.220174681684565	21.68756497313939	25.61070212742783
48	29.165491754638833	23.682999254914158	21.281620261171348	25.869888729275658
49	29.51217652604432	22.47113548742612	21.592603843061607	26.42408414346795
50	29.186428384422342	23.425385925824628	21.824762425728412	25.563423264024614
51	28.052260086997947	24.64660864920818	22.124571737002043	25.176559526791824
52	29.892182407664013	24.18676926018664	20.9574857326348	24.963562599514542
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	1.5
8	2.0
9	53.5
10	105.0
11	386.0
12	667.0
13	1211.0
14	2033.0
15	2311.0
16	3002.5
17	3694.0
18	4039.0
19	4384.0
20	5010.0
21	5636.0
22	7156.0
23	8676.0
24	11018.0
25	13360.0
26	16397.5
27	19435.0
28	22692.0
29	25949.0
30	29237.5
31	32526.0
32	34379.5
33	36233.0
34	37351.0
35	38469.0
36	38551.5
37	38634.0
38	38228.5
39	39779.0
40	41735.0
41	44976.5
42	48218.0
43	48872.0
44	49526.0
45	56803.0
46	64080.0
47	69302.0
48	74524.0
49	89178.5
50	103833.0
51	110379.5
52	116926.0
53	130372.5
54	143819.0
55	154612.5
56	165406.0
57	182204.5
58	199003.0
59	205996.0
60	212989.0
61	214872.5
62	216756.0
63	199188.0
64	176095.5
65	170571.0
66	149397.0
67	128223.0
68	111592.5
69	94962.0
70	90060.5
71	85159.0
72	68878.0
73	52597.0
74	41311.5
75	30026.0
76	23668.0
77	17310.0
78	13279.0
79	9248.0
80	5220.5
81	1193.0
82	1155.0
83	1117.0
84	643.5
85	170.0
86	163.5
87	157.0
88	117.0
89	40.0
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2478909.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.021826935361092
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.63689577158706	11.306765971139447
2	6.6434411989482385	1.8630636549380002
3	3.043331167562836	1.280191888156695
4	1.802670104880043	1.011069129287088
5	1.2218983023438603	0.8566623264038568
6	0.851268003670403	0.7161799573846035
7	0.5984587067787436	0.5874039090088088
8	0.4648937282919252	0.5214927521155327
9	0.3361270648581583	0.4241803978558791
>10	2.0188563625780507	5.304248911911957
>50	0.4281191732923943	4.499031378217968
>100	1.8087430403932014	57.68842997416805
>500	0.132740811559387	11.787112767068702
>1k	0.012556563255617688	2.154166982343425
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	8.874871969886752E-4	0.0	0.0	0.0	0.0
12	0.006373771687464122	0.0	0.0	0.0	0.0
13	0.028157548340822514	0.0	0.0	0.0	0.0
14	0.08189086408577322	0.0	0.0	0.0	0.0
15	0.1484927441870597	0.0	0.0	0.0	0.0
16	0.192463700765135	0.0	0.0	0.0	0.0
17	0.22029852648887072	0.0	0.0	0.0	0.0
18	0.25446678357293473	0.0	0.0	0.0	0.0
19	0.28577087743035345	0.0	0.0	0.0	0.0
20	0.3180834794661684	0.0	0.0	0.0	0.0
21	0.3455149019185456	0.0	0.0	0.0	0.0
22	0.3812967720880436	0.0	0.0	0.0	0.0
23	0.42187914118670755	0.0	0.0	0.0	0.0
24	0.4679074544487111	0.0	0.0	0.0	0.0
25	0.5187766069670166	0.0	0.0	0.0	0.0
26	0.5752934052843408	0.0	0.0	0.0	0.0
27	0.632092585891616	0.0	0.0	0.0	0.0
28	0.6903843586029177	0.0	0.0	0.0	0.0
29	0.7525084623921249	0.0	0.0	0.0	0.0
30	0.8215711024486982	0.0	0.0	0.0	0.0
31	0.8833724836208187	0.0	0.0	0.0	0.0
32	0.9394051980125128	0.0	0.0	0.0	0.0
33	0.9929368121217842	0.0	0.0	0.0	0.0
34	1.0428781371159652	0.0	0.0	0.0	0.0
35	1.0866473920583613	0.0	0.0	0.0	0.0
36	1.1292467775138175	0.0	0.0	0.0	0.0
37	1.1689416594154929	0.0	0.0	0.0	0.0
38	1.2088785832799833	0.0	0.0	0.0	0.0
39	1.2514376284083037	0.0	0.0	0.0	0.0
40	1.2989585337743337	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCATAAG	40	5.6152203E-9	46.000004	44
AACCGTA	20	6.3128734E-4	46.000004	18
AACGTAC	20	6.3128734E-4	46.000004	40
CAAACTA	35	1.0202166E-7	46.000004	33
AAATCGT	20	6.3128734E-4	46.000004	35
CGAACCT	35	1.0202166E-7	46.000004	26
TACCATA	20	6.3128734E-4	46.000004	36
CTATCGA	35	1.0202166E-7	46.000004	43
CGTGCAA	20	6.3128734E-4	46.000004	21
GACGTAA	20	6.3128734E-4	46.000004	43
CCGGATA	35	1.0202166E-7	46.000004	24
TAAACCC	20	6.3128734E-4	46.000004	29
GTGATAG	20	6.3128734E-4	46.000004	23
CGATTTC	75	0.0	46.000004	35
GTCTAAC	20	6.3128734E-4	46.000004	42
CAATTCA	20	6.3128734E-4	46.000004	26
TAGCCTA	20	6.3128734E-4	46.000004	38
TTATCGT	20	6.3128734E-4	46.000004	27
CGTTCGA	35	1.0202166E-7	46.000004	23
CGTTAAC	20	6.3128734E-4	46.000004	29
>>END_MODULE