FastQCFastQC Report
Fri 17 Jun 2016
SRR1527913_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527913_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2475709
Sequences flagged as poor quality0
Sequence length59
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTA1200.053.00000423
AACCGTA307.0137867E-753.00000418
TTATGCG150.006697173753.00000429
TTATCCG550.053.00000427
CGTTAGT550.053.00000449
ACGATTA150.006697173753.00000431
CTACGTA150.006697173753.00000444
TAACATA307.0137867E-753.00000449
TCGTACA150.006697173753.00000423
GATTAAT150.006697173753.00000444
TACGTAG150.006697173753.00000445
CTTACGA307.0137867E-753.00000441
ACGGTCA307.0137867E-753.00000425
TAATCGT150.006697173753.00000420
TTTAGCG203.1389535E-453.039
ATTAGGC203.1389535E-453.034
CGCATTG203.1389535E-453.017
GACGTAA503.6379788E-1253.043
CAATTAC353.344212E-853.041
CCTAATA251.4792324E-553.018