FastQCFastQC Report
Fri 17 Jun 2016
SRR1527912_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527912_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5869280
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGG501.6370905E-1146.034
CGTACCG253.4188837E-546.025
TATAGCG301.8627197E-646.028
TATCGCT253.4188837E-546.046
TATCGCG253.4188837E-546.014
TGCGTAA206.313584E-446.034
TGGTAGG5960850.044.4778186
TGTGGTA1980350.044.4379044
GGTAGGG5868700.044.419437
CGTGGTA1248650.044.415894
GTAGGGA1434450.044.3581168
GTAGGGC665050.044.3365148
GTGGTAG6039100.044.2934075
GTAGGGG3327800.044.278358
TTGTGGT712800.044.2059483
TAGGGGG1272950.044.1498079
TAGGGGC755100.044.1358769
TAGGGAC224350.044.072669
TAGGGAT388950.044.048599
TAGGGGA844150.043.9674229
Produced by FastQC (version 0.11.5)