##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527912_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5869280
Sequences flagged as poor quality	0
Sequence length	52
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.266075055202684	31.0	31.0	33.0	30.0	33.0
2	31.81763163454461	31.0	31.0	34.0	30.0	34.0
3	32.29842672355042	33.0	31.0	34.0	30.0	34.0
4	35.9834090382466	37.0	35.0	37.0	35.0	37.0
5	36.10159355150887	37.0	35.0	37.0	35.0	37.0
6	33.93634057329008	35.0	33.0	35.0	31.0	37.0
7	35.214030852165855	35.0	35.0	37.0	33.0	37.0
8	35.714553233105256	37.0	35.0	37.0	35.0	37.0
9	34.92916422457269	37.0	34.0	37.0	31.0	39.0
10	36.82999447973176	37.0	37.0	39.0	34.0	39.0
11	37.77779318758008	39.0	37.0	39.0	35.0	39.0
12	38.13946276204236	39.0	39.0	39.0	37.0	39.0
13	38.181646811874714	39.0	39.0	39.0	37.0	39.0
14	38.681412711610285	40.0	39.0	40.0	36.0	40.0
15	38.11570362974675	40.0	38.0	40.0	34.0	40.0
16	37.52753421203282	39.0	36.0	40.0	33.0	40.0
17	37.55691481749039	40.0	37.0	40.0	33.0	40.0
18	37.480662193659185	40.0	37.0	40.0	33.0	40.0
19	37.62574216939727	40.0	37.0	40.0	33.0	40.0
20	37.66459242019464	40.0	37.0	40.0	33.0	40.0
21	37.399520724858924	40.0	36.0	40.0	33.0	40.0
22	37.48590150751029	40.0	36.0	40.0	33.0	40.0
23	37.45269317531282	40.0	36.0	40.0	33.0	40.0
24	37.38407351497969	40.0	36.0	40.0	33.0	40.0
25	37.6391886227953	40.0	37.0	40.0	33.0	40.0
26	37.70286116866123	40.0	37.0	40.0	33.0	40.0
27	37.752460267698936	40.0	37.0	40.0	34.0	40.0
28	37.744249550200365	40.0	37.0	40.0	34.0	40.0
29	37.78634568464957	40.0	37.0	40.0	34.0	40.0
30	37.775209906496194	40.0	37.0	40.0	34.0	40.0
31	37.763768809802905	40.0	37.0	40.0	34.0	40.0
32	37.721550513862006	40.0	37.0	40.0	34.0	40.0
33	37.68611431044353	40.0	37.0	40.0	34.0	40.0
34	37.637992394297086	40.0	37.0	40.0	33.0	40.0
35	37.558702259902404	40.0	36.0	40.0	33.0	40.0
36	37.54582878990268	40.0	36.0	40.0	33.0	40.0
37	37.43970623313251	40.0	36.0	40.0	33.0	40.0
38	37.27718851375297	40.0	35.0	40.0	33.0	40.0
39	37.21027451408009	40.0	35.0	40.0	33.0	40.0
40	37.138897956819235	40.0	35.0	40.0	33.0	40.0
41	37.06494305945533	39.0	35.0	40.0	33.0	40.0
42	36.95632650001363	39.0	35.0	40.0	32.0	40.0
43	36.8478932679988	39.0	35.0	40.0	32.0	40.0
44	36.74008634789957	39.0	35.0	40.0	32.0	40.0
45	36.60724705585694	39.0	35.0	40.0	32.0	40.0
46	36.49518816618052	39.0	35.0	40.0	31.0	40.0
47	36.41046516778889	38.0	35.0	40.0	31.0	40.0
48	36.28234945342529	38.0	35.0	40.0	31.0	40.0
49	36.170355478014336	38.0	35.0	40.0	31.0	40.0
50	36.0517051495243	38.0	35.0	40.0	31.0	40.0
51	35.91490438350189	37.0	35.0	40.0	31.0	40.0
52	35.2401817599433	36.0	34.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	5.0
14	11.0
15	24.0
16	69.0
17	253.0
18	518.0
19	912.0
20	1595.0
21	2811.0
22	4928.0
23	7831.0
24	11528.0
25	16847.0
26	24271.0
27	34506.0
28	47614.0
29	65847.0
30	88138.0
31	116934.0
32	155041.0
33	210346.0
34	347884.0
35	523176.0
36	516715.0
37	938059.0
38	2224169.0
39	529248.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.99872897527465	18.224041108960552	31.19917604885097	18.57805386691383
2	31.1446719188725	18.719399994547885	31.211034402856907	18.924893683722704
3	22.177490254341247	22.61877095657389	33.8402495706458	21.363489218439057
4	26.342106697925473	20.256726549082682	32.61498855055475	20.786178202437096
5	100.0	0.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	100.0	0.0	0.0	0.0
8	100.0	0.0	0.0	0.0
9	0.0	0.0	100.0	0.0
10	0.0	100.0	0.0	0.0
11	99.14279093858191	0.730140664613036	0.10948532017555816	0.017583076629501405
12	99.05461998746013	0.7330711773846196	0.10749188997628328	0.10481694517896574
13	98.15616225499551	1.0173820298230787	0.303512526238312	0.5229431889431072
14	56.3641707330371	24.4643806395333	7.889843387945369	11.28160523948423
15	34.27135866750266	27.35245208952376	18.63490922225554	19.741280020718044
16	29.336920371834363	19.077791483793582	26.074987051222635	25.51030109314942
17	23.693246871847997	18.721206008232695	28.038822479077503	29.54672464084181
18	22.49025434124799	19.884449199901862	30.877875991603744	26.747420467246407
19	25.39313169588093	21.237664585775427	28.486509418531746	24.882694299811902
20	29.953622931603196	20.9650076329635	25.500538396532452	23.580831038900854
21	30.675892102608838	21.35938309298585	23.724818035602322	24.23990676880299
22	29.961954447564267	21.85961480794919	23.876523185126626	24.301907559359922
23	29.163031922143773	22.44716558078674	23.03672682168852	25.353075675380964
24	28.99539636889022	23.469011531227	22.872566992884988	24.66302510699779
25	28.95516996974075	22.57341616007415	24.802428918027424	23.668984952157675
26	28.297729874873923	21.128809666603058	24.720323446828232	25.853137011694788
27	28.28353733336968	22.57844233023471	24.28415410408091	24.853866232314697
28	28.443079900771473	22.474715808412615	23.16144399313033	25.920760297685575
29	29.176270343210753	21.95230079328299	22.329791729138837	26.54163713436742
30	26.874471826186518	22.575648120382738	22.72396273478178	27.825917318648962
31	26.05570018809803	21.881440312951504	23.792509473052913	28.270350025897557
32	26.685181146580163	23.360752937327916	22.750899599269417	27.203166316822507
33	28.100635853119975	23.698460458523023	22.51436292015375	25.686540768203255
34	28.09767126461849	22.41394174413216	23.838545784150696	25.649841207098657
35	29.31846836409236	22.245454297631056	22.80301842815473	25.633058910121854
36	31.27981626366437	21.96668075130169	22.499505901916418	24.253997083117522
37	30.877279666330455	21.95211337676853	22.48577338276586	24.68483357413516
38	29.019573099255787	23.1034471008369	24.577512062808385	23.29946773709893
39	27.721969304582505	25.059973284627755	23.92724490908595	23.290812501703787
40	28.080105225853934	26.02349862334051	22.330575470926586	23.565820679878964
41	28.93808099119483	24.732011422184662	21.945315268653054	24.384592317967453
42	28.97285527355996	24.75859049150833	21.903828067497205	24.364726167434505
43	28.73369476324183	23.63156980072513	21.647169669874327	25.98756576615871
44	28.490002180846712	23.843997900935037	22.302854864651202	25.36314505356705
45	26.260529400539763	24.438159365373608	22.451237630510047	26.85007360357659
46	25.32474170596734	24.025825995692827	23.277335550527493	27.372096747812336
47	26.512008287217515	22.038035329716763	23.641996974075184	27.80795940899054
48	27.197577897118556	22.33941812283619	22.731408281765393	27.73159569827986
49	26.82298339830439	22.390293187580077	22.985562113240466	27.801161300875066
50	27.01007619333206	23.628775590873158	22.47652182209743	26.884626393697353
51	25.572608565275466	23.479506856036856	24.557765177330097	26.390119401357577
52	26.435457159992364	23.171666712100976	24.009316304555245	26.383559823351415
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	4.5
10	8.0
11	69.5
12	131.0
13	275.5
14	660.0
15	900.0
16	1199.5
17	1499.0
18	1992.5
19	2486.0
20	3196.0
21	3906.0
22	4506.5
23	5107.0
24	6135.0
25	7163.0
26	9486.5
27	11810.0
28	15438.0
29	19066.0
30	23544.5
31	28023.0
32	34001.0
33	39979.0
34	48635.5
35	57292.0
36	66240.0
37	75188.0
38	90798.5
39	115956.0
40	125503.0
41	134906.0
42	144309.0
43	168779.0
44	193249.0
45	207671.0
46	222093.0
47	249754.5
48	277416.0
49	299150.5
50	320885.0
51	359272.0
52	397659.0
53	416627.0
54	435595.0
55	467041.5
56	498488.0
57	497624.0
58	496760.0
59	502486.5
60	508213.0
61	506387.5
62	504562.0
63	459764.0
64	373446.0
65	331926.0
66	274955.0
67	217984.0
68	181732.0
69	145480.0
70	124941.0
71	104402.0
72	89423.0
73	74444.0
74	63803.0
75	53162.0
76	38776.5
77	24391.0
78	16937.5
79	9484.0
80	7406.0
81	5328.0
82	3674.0
83	2020.0
84	1276.0
85	532.0
86	379.0
87	226.0
88	223.0
89	270.0
90	320.0
91	297.5
92	275.0
93	137.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	5869280.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	5.554287682839474
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.02123717744041	3.7780951982615982
2	9.199911490604935	1.0219791015096067
3	4.66218192611747	0.7768529894217325
4	2.852558253599955	0.6337571669020925
5	1.7767696635462287	0.49343449287388275
6	1.2206596250955868	0.4067936832364722
7	0.798284266124453	0.31037303267177196
8	0.5728129754379565	0.2545254443236538
9	0.3973974129703858	0.19865336004483172
>10	3.285626307395876	4.099116654966513
>50	1.631507146381566	6.78156258291303
>100	5.1397783562774055	64.30033731278365
>500	0.41041697949669054	14.741096488500851
>1k	0.030858419511029363	2.2034224915903393
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.022575171060164108	0.0	0.0	0.0	0.0
11	0.023410026442766404	0.0	0.0	0.0	0.0
12	0.024227843960417633	0.0	0.0	0.0	0.0
13	0.026170160564839298	0.0	0.0	0.0	0.0
14	0.03092372488618706	0.0	0.0	0.0	0.0
15	0.04373619932938964	0.0	0.0	0.0	0.0
16	0.04910312678897582	0.0	0.0	0.0	0.0
17	0.053890766840225715	0.0	0.0	0.0	0.0
18	0.059871057438050324	0.0	0.0	0.0	0.0
19	0.06772551318049233	0.0	0.0	0.0	0.0
20	0.07529032521876619	0.0	0.0	0.0	0.0
21	0.08202028187443774	0.0	0.0	0.0	0.0
22	0.09013030559114577	0.0	0.0	0.0	0.0
23	0.10011449445247117	0.0	0.0	0.0	0.0
24	0.10865046479295587	0.0	0.0	0.0	0.0
25	0.1174760788376087	0.0	0.0	0.0	0.0
26	0.1267446773709893	0.0	0.0	0.0	0.0
27	0.13630291960853802	0.0	0.0	0.0	0.0
28	0.14579301038628248	0.0	0.0	0.0	0.0
29	0.15731060709320394	0.0	0.0	0.0	0.0
30	0.1706001417550364	0.0	0.0	0.0	0.0
31	0.18237330643622388	0.0	0.0	0.0	0.0
32	0.19276640405637488	0.0	0.0	0.0	0.0
33	0.20402843278903035	0.0	0.0	0.0	0.0
34	0.215341575116539	0.0	0.0	0.0	0.0
35	0.22525761251806015	0.0	0.0	0.0	0.0
36	0.23558255867840688	0.0	0.0	1.703786495106725E-5	0.0
37	0.24498746013139602	0.0	0.0	1.703786495106725E-5	0.0
38	0.25614726167434504	0.0	0.0	1.703786495106725E-5	0.0
39	0.2717539459695227	0.0	0.0	1.703786495106725E-5	0.0
40	0.29257421693972685	0.0	0.0	1.703786495106725E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCGG	50	1.6370905E-11	46.0	34
CGTACCG	25	3.4188837E-5	46.0	25
TATAGCG	30	1.8627197E-6	46.0	28
TATCGCT	25	3.4188837E-5	46.0	46
TATCGCG	25	3.4188837E-5	46.0	14
TGCGTAA	20	6.313584E-4	46.0	34
TGGTAGG	596085	0.0	44.477818	6
TGTGGTA	198035	0.0	44.437904	4
GGTAGGG	586870	0.0	44.41943	7
CGTGGTA	124865	0.0	44.41589	4
GTAGGGA	143445	0.0	44.358116	8
GTAGGGC	66505	0.0	44.336514	8
GTGGTAG	603910	0.0	44.293407	5
GTAGGGG	332780	0.0	44.27835	8
TTGTGGT	71280	0.0	44.205948	3
TAGGGGG	127295	0.0	44.149807	9
TAGGGGC	75510	0.0	44.135876	9
TAGGGAC	22435	0.0	44.07266	9
TAGGGAT	38895	0.0	44.04859	9
TAGGGGA	84415	0.0	43.967422	9
>>END_MODULE