FastQCFastQC Report
Fri 17 Jun 2016
SRR1527911_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527911_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5828143
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACCG351.0205258E-746.00000425
CGTATCG253.4188804E-546.018
TGGTAGG5924650.044.4995736
GGTAGGG5832500.044.436047
TGTGGTA1972850.044.4342964
GTAGGGC658000.044.3711288
CGTGGTA1250500.044.370414
GTAGGGA1429850.044.3158348
GTAGGGG3300000.044.2924238
GTGGTAG6003400.044.2878535
TAGGGAC219150.044.268319
TTGTGGT719500.044.161923
TAGGGGC744900.044.1165249
TAGGGGG1270900.044.1015789
TAGGGGA821850.044.0941739
TAGTGGT395050.043.979753
TAGGGAT387250.043.9687549
TAGGGCA308600.043.846089
TAGGGGT479500.043.8271149
ACGTGGT288000.043.819793
Produced by FastQC (version 0.11.5)