##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527911_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5828143
Sequences flagged as poor quality	0
Sequence length	52
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.224065023799177	31.0	31.0	33.0	30.0	33.0
2	31.655049644457936	31.0	31.0	34.0	30.0	34.0
3	32.22329633984616	33.0	31.0	34.0	30.0	34.0
4	35.95124879399836	37.0	35.0	37.0	35.0	37.0
5	36.085237785002874	37.0	35.0	37.0	35.0	37.0
6	33.86404057004092	35.0	33.0	35.0	31.0	37.0
7	35.202982665318956	35.0	35.0	37.0	33.0	37.0
8	35.64557372734334	37.0	35.0	37.0	35.0	37.0
9	34.83837596297826	37.0	34.0	37.0	31.0	39.0
10	36.78399191646464	37.0	37.0	39.0	34.0	39.0
11	37.73882538571892	39.0	37.0	39.0	35.0	39.0
12	38.098303181648085	39.0	38.0	39.0	37.0	39.0
13	38.18236391934789	39.0	39.0	39.0	37.0	39.0
14	38.70469598978611	40.0	39.0	40.0	36.0	40.0
15	38.13954444837747	40.0	38.0	40.0	34.0	40.0
16	37.5639988586416	39.0	36.0	40.0	33.0	40.0
17	37.60532917603429	40.0	37.0	40.0	33.0	40.0
18	37.55378960330932	40.0	37.0	40.0	33.0	40.0
19	37.690611057415715	40.0	37.0	40.0	33.0	40.0
20	37.7084503588879	40.0	37.0	40.0	33.0	40.0
21	37.63738415478138	40.0	37.0	40.0	33.0	40.0
22	37.61853269557731	40.0	37.0	40.0	33.0	40.0
23	37.55697689641452	40.0	37.0	40.0	33.0	40.0
24	37.49327821915145	40.0	36.0	40.0	33.0	40.0
25	37.43089711422661	40.0	36.0	40.0	33.0	40.0
26	37.57208651194729	40.0	37.0	40.0	33.0	40.0
27	37.6908780378244	40.0	37.0	40.0	33.0	40.0
28	37.547725579142444	40.0	37.0	40.0	33.0	40.0
29	37.697360720215684	40.0	37.0	40.0	34.0	40.0
30	37.752629954343945	40.0	37.0	40.0	34.0	40.0
31	37.78348763920172	40.0	37.0	40.0	34.0	40.0
32	37.73869944508911	40.0	37.0	40.0	34.0	40.0
33	37.70011751599094	40.0	37.0	40.0	34.0	40.0
34	37.67164378087497	40.0	37.0	40.0	34.0	40.0
35	37.52630194557683	40.0	36.0	40.0	33.0	40.0
36	37.1430845125111	40.0	35.0	40.0	33.0	40.0
37	37.25550385431517	40.0	35.0	40.0	33.0	40.0
38	37.20752133226656	40.0	35.0	40.0	33.0	40.0
39	37.1802817810064	40.0	35.0	40.0	33.0	40.0
40	37.117688944832	39.0	35.0	40.0	33.0	40.0
41	37.0755079276538	39.0	35.0	40.0	33.0	40.0
42	36.820015226119196	39.0	35.0	40.0	32.0	40.0
43	36.724626008661765	39.0	35.0	40.0	32.0	40.0
44	36.62254838290687	39.0	35.0	40.0	32.0	40.0
45	36.24671735062094	39.0	35.0	40.0	31.0	40.0
46	36.291741125775395	38.0	35.0	40.0	31.0	40.0
47	36.281271753284024	38.0	35.0	40.0	31.0	40.0
48	36.15284422499585	38.0	35.0	40.0	31.0	40.0
49	36.042114100494786	38.0	35.0	40.0	31.0	40.0
50	35.85892641961599	38.0	35.0	40.0	30.0	40.0
51	35.813590366605624	37.0	35.0	40.0	30.0	40.0
52	35.29709446044821	37.0	34.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	4.0
13	4.0
14	14.0
15	26.0
16	88.0
17	226.0
18	549.0
19	932.0
20	1573.0
21	2853.0
22	4933.0
23	7864.0
24	11781.0
25	17516.0
26	25213.0
27	35477.0
28	49608.0
29	67269.0
30	90200.0
31	119814.0
32	158688.0
33	215691.0
34	352884.0
35	521319.0
36	527234.0
37	954386.0
38	2154649.0
39	507348.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.995440056978698	18.214927121726422	31.173531603462717	18.616101217832163
2	31.214968472805143	18.74219627075039	31.113392379013348	18.929442877431114
3	22.18277760171636	22.585718984589086	33.88715753885929	21.34434587483526
4	26.354466594247945	20.235690853844872	32.62667027902369	20.78317227288349
5	100.0	0.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	100.0	0.0	0.0	0.0
8	100.0	0.0	0.0	0.0
9	0.0	0.0	100.0	0.0
10	0.0	100.0	0.0	0.0
11	99.13504181349016	0.7346250769756335	0.11120180132848491	0.019131308205718355
12	99.05717824699909	0.728911421699845	0.10816481338910182	0.10574551791196612
13	98.14965075496603	1.0221780762757537	0.3021202465347882	0.5260509222234252
14	56.30301109633034	24.490099161945754	7.899600267186306	11.307289474537601
15	34.29327317466301	27.36834013853126	18.627322630896327	19.711064055909404
16	29.30025910482979	19.10292866870288	26.078632593606578	25.51817963286076
17	23.717400207922147	18.694222156182512	28.04298041417309	29.545397221722254
18	22.47758848744789	19.880689269292123	30.857478960279455	26.78424328298053
19	25.383265304231557	21.2536480316286	28.510796663705744	24.852290000434103
20	29.98689290911359	21.01679728860462	25.453750877423566	23.542558924858227
21	30.378080977079662	21.428883951543398	23.785878280611854	24.407156790765086
22	30.034232173095273	21.838619951500846	23.879046893667503	24.24810098173638
23	29.165773729299367	22.47436276014504	23.012424369134386	25.3474391414212
24	28.94144841675985	23.497741905097385	22.88528267065513	24.675527007487634
25	28.967597397661653	22.57470346901234	24.76287901652379	23.694820116802212
26	28.340279227877556	21.143784563968318	24.708093126747233	25.80784308140689
27	28.274374873780552	22.585547403349572	24.270852654095822	24.86922506877405
28	28.569906400718033	22.46106521408277	23.158422845836142	25.810605539363053
29	29.190601534656924	21.987792681133598	22.296467331017787	26.525138453191694
30	26.86217548196741	22.622711899828126	22.710475703839116	27.80463691436535
31	26.052003185234128	21.90706370794265	23.7636928263428	28.27724028048042
32	26.65248604915837	23.41883169304528	22.742990348726856	27.185691909069494
33	28.13354099238814	23.68709896102412	22.545140021444222	25.634220025143517
34	28.114152312323153	22.424998837537103	23.8431692564853	25.617679593654447
35	29.305286435147526	22.226324920304805	22.81376761002604	25.65462103452163
36	31.32280728183917	21.92243738700303	22.560273486769287	24.194481844388513
37	30.84555406413329	21.978527294199885	22.49481524389501	24.68110339777181
38	29.069842658287552	23.072546435459802	24.551061976344783	23.306548929907862
39	27.702014175012522	25.066766549825566	23.936252078921193	23.294967196240723
40	28.052537489213975	26.013620461955036	22.35497653369178	23.578865515139213
41	28.91469890151975	24.79245962221586	21.892290563220566	24.40055091304383
42	29.052375688105116	24.777566370626115	21.879353337761273	24.2907046035075
43	28.705301156817875	23.637666405920378	21.661445163579547	25.9955872736822
44	28.67542886301863	23.800308262854912	22.188800103223276	25.335462770903185
45	26.352664991233056	24.469938366302955	22.438433648590983	26.738962993873006
46	25.34127937492268	24.075936366008865	23.283351146325682	27.299433112742772
47	26.505732615002753	22.032300854663312	23.6477382246798	27.814228305654133
48	27.18538306283837	22.336926187294992	22.760697532644617	27.71699321722202
49	26.87322531379206	22.377487992315906	22.992486629102956	27.75680006478908
50	27.110453535542966	23.60880644143426	22.475735410061144	26.805004612961625
51	25.557900689808054	23.479571451832943	24.565560591083642	26.396967267275357
52	26.444924223719283	23.16504588168135	23.98573610839679	26.404293786202572
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.5
8	3.0
9	8.0
10	13.0
11	65.0
12	117.0
13	259.5
14	628.5
15	855.0
16	1160.0
17	1465.0
18	1973.0
19	2481.0
20	3134.5
21	3788.0
22	4328.5
23	4869.0
24	6026.5
25	7184.0
26	9559.5
27	11935.0
28	15329.5
29	18724.0
30	23268.5
31	27813.0
32	33639.5
33	39466.0
34	48278.5
35	57091.0
36	65799.0
37	74507.0
38	89866.5
39	115480.5
40	125735.0
41	134885.5
42	144036.0
43	168337.0
44	192638.0
45	206898.5
46	221159.0
47	247934.0
48	274709.0
49	297030.5
50	319352.0
51	356848.0
52	394344.0
53	414082.0
54	433820.0
55	463670.5
56	493521.0
57	493254.5
58	492988.0
59	498604.5
60	504221.0
61	501753.5
62	499286.0
63	455370.5
64	370585.0
65	329715.0
66	273319.5
67	216924.0
68	180809.5
69	144695.0
70	124467.0
71	104239.0
72	89209.0
73	74179.0
74	63346.0
75	52513.0
76	38405.0
77	24297.0
78	16854.5
79	9412.0
80	7390.5
81	5369.0
82	3726.5
83	2084.0
84	1317.0
85	550.0
86	369.5
87	189.0
88	190.0
89	253.5
90	316.0
91	291.5
92	267.0
93	133.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	5828143.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	5.869001610433268
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.43211192269091	3.9575917346918925
2	9.810880618001292	1.1516014829363623
3	4.877397167860525	0.8587635549868824
4	2.8584900674411817	0.6710593123687919
5	1.796997734773874	0.52732912996664
6	1.1939954266580874	0.4204536649143764
7	0.7940296125494021	0.32621127523489013
8	0.5872793222162134	0.2757394630288892
9	0.40447256293783906	0.2136465110932424
>10	3.2259762812444976	4.306597946926986
>50	1.6675263397457067	7.351308126414328
>100	4.970856428508603	64.67194216646587
>500	0.3553436749034632	13.418380908603384
>1k	0.024642840468476472	1.849374722367466
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.02161923617865931	0.0	0.0	0.0	0.0
11	0.02256293299598174	0.0	0.0	0.0	0.0
12	0.02299188609476466	0.0	0.0	0.0	0.0
13	0.025033702844971376	0.0	0.0	0.0	0.0
14	0.030215456278269083	0.0	0.0	0.0	0.0
15	0.042758044886681744	0.0	0.0	0.0	0.0
16	0.04802558893973603	0.0	0.0	0.0	0.0
17	0.05317302612513111	0.0	0.0	0.0	0.0
18	0.05940142511945915	0.0	0.0	0.0	0.0
19	0.0666593115508662	0.0	0.0	0.0	0.0
20	0.07398583047807852	0.0	0.0	0.0	0.0
21	0.0818785674956843	0.0	0.0	0.0	0.0
22	0.08994288575280325	0.0	0.0	0.0	0.0
23	0.10075250384213291	0.0	0.0	0.0	0.0
24	0.10871387335554396	0.0	0.0	0.0	0.0
25	0.11755030719047216	0.0	0.0	0.0	0.0
26	0.12705590785950174	0.0	0.0	0.0	0.0
27	0.13623550417345628	0.0	0.0	0.0	0.0
28	0.14606710919756086	0.0	0.0	0.0	0.0
29	0.15684241103898788	0.0	0.0	0.0	0.0
30	0.1699169014898914	0.0	0.0	0.0	0.0
31	0.1812069470498579	0.0	0.0	0.0	0.0
32	0.1916562445362099	0.0	0.0	0.0	0.0
33	0.2026031276171501	0.0	0.0	0.0	0.0
34	0.21272642074842707	0.0	0.0	0.0	0.0
35	0.2233129832263896	0.0	0.0	0.0	0.0
36	0.23360785759717975	0.0	0.0	5.147437185395074E-5	0.0
37	0.24362820198474883	0.0	0.0	5.147437185395074E-5	0.0
38	0.2554673075111575	0.0	0.0	5.147437185395074E-5	0.0
39	0.2710125678110506	0.0	0.0	5.147437185395074E-5	0.0
40	0.2921170602711704	0.0	0.0	5.147437185395074E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTACCG	35	1.0205258E-7	46.000004	25
CGTATCG	25	3.4188804E-5	46.0	18
TGGTAGG	592465	0.0	44.499573	6
GGTAGGG	583250	0.0	44.43604	7
TGTGGTA	197285	0.0	44.434296	4
GTAGGGC	65800	0.0	44.371128	8
CGTGGTA	125050	0.0	44.37041	4
GTAGGGA	142985	0.0	44.315834	8
GTAGGGG	330000	0.0	44.292423	8
GTGGTAG	600340	0.0	44.287853	5
TAGGGAC	21915	0.0	44.26831	9
TTGTGGT	71950	0.0	44.16192	3
TAGGGGC	74490	0.0	44.116524	9
TAGGGGG	127090	0.0	44.101578	9
TAGGGGA	82185	0.0	44.094173	9
TAGTGGT	39505	0.0	43.97975	3
TAGGGAT	38725	0.0	43.968754	9
TAGGGCA	30860	0.0	43.84608	9
TAGGGGT	47950	0.0	43.827114	9
ACGTGGT	28800	0.0	43.81979	3
>>END_MODULE