FastQCFastQC Report
Fri 17 Jun 2016
SRR1527910_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527910_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5762370
Sequences flagged as poor quality0
Sequence length59
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG203.139304E-453.027
CGTAACA457.6397555E-1153.016
TGGTAGG5851650.051.2514956
TGTGGTA1932600.051.2489664
GGTAGGG5760050.051.18787
GTAGGGG3260050.051.106018
CGTGGTA1241400.051.0659754
GTAGGGA1418900.051.0371068
GTGGTAG5932000.051.0057955
GTAGGGC650850.050.9967738
TAGGGGG1262100.050.927629
TAGGGGA827950.050.8811539
TTGTGGT680750.050.8239483
TAGGGAT383700.050.7346889
TAGGGGC731150.050.6332479
TAGTGGT392550.050.616993
TAGGGGT458700.050.544699
TAGGGAC223600.050.4874769
TAGGGCA305150.050.3339359
ACGTGGT282400.050.297453
Produced by FastQC (version 0.11.5)