FastQCFastQC Report
Fri 17 Jun 2016
SRR1527909_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527909_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3590321
Sequences flagged as poor quality0
Sequence length52
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTA301.862516E-646.030
CGTATAT206.3132547E-446.025
TTATGCG301.862516E-646.021
TAACGTA301.862516E-646.043
CCGTATA206.3132547E-446.024
CCGTAAA301.862516E-646.046
CGTACAC453.110472E-1046.040
TCGTAAT253.418616E-546.034
ACGTCGA253.418616E-546.026
CATAACG253.418616E-546.019
CGTAAAC405.6170393E-946.038
ATACGTA253.418616E-546.017
ATACGCT551.8189894E-1246.026
TTCGATA253.418616E-546.030
TCGGTAC253.418616E-546.036
TACGTTG253.418616E-546.046
GTACGTT301.862516E-646.020
CTAATAG301.862516E-646.043
TACGTAC206.3132547E-446.018
TAATCGC453.110472E-1046.018
Produced by FastQC (version 0.11.5)