##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527909_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3590321
Sequences flagged as poor quality	0
Sequence length	52
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.95909697210918	33.0	31.0	34.0	30.0	34.0
2	32.40945586759513	34.0	31.0	34.0	30.0	34.0
3	32.51993958200395	34.0	31.0	34.0	31.0	34.0
4	36.05897801338655	37.0	35.0	37.0	35.0	37.0
5	36.0948480651173	37.0	35.0	37.0	35.0	37.0
6	36.171531737691424	37.0	36.0	37.0	35.0	37.0
7	36.32136235172287	37.0	37.0	37.0	35.0	37.0
8	36.13280901624117	37.0	37.0	37.0	35.0	37.0
9	37.21219049772987	39.0	37.0	39.0	33.0	39.0
10	37.88784234055952	39.0	38.0	39.0	35.0	39.0
11	38.24823964208214	39.0	39.0	39.0	37.0	39.0
12	38.22547733197115	39.0	39.0	39.0	37.0	39.0
13	38.1890828146007	39.0	39.0	39.0	37.0	39.0
14	38.83137050976779	40.0	39.0	40.0	36.0	40.0
15	38.27402591578859	40.0	38.0	40.0	35.0	40.0
16	37.69678310100963	40.0	37.0	40.0	33.0	40.0
17	37.65137295523158	40.0	37.0	40.0	33.0	40.0
18	37.67728595855357	40.0	37.0	40.0	33.0	40.0
19	37.80044291304315	40.0	37.0	40.0	33.0	40.0
20	37.85534608186845	40.0	37.0	40.0	33.0	40.0
21	37.653969380453724	40.0	37.0	40.0	33.0	40.0
22	37.7324835300242	40.0	37.0	40.0	33.0	40.0
23	37.67872204184528	40.0	37.0	40.0	33.0	40.0
24	37.64514844215879	40.0	37.0	40.0	33.0	40.0
25	37.74982654754268	40.0	37.0	40.0	33.0	40.0
26	37.75045239687482	40.0	37.0	40.0	33.0	40.0
27	37.73547908390364	40.0	37.0	40.0	33.0	40.0
28	37.72642891819422	40.0	37.0	40.0	33.0	40.0
29	37.72777782265151	40.0	37.0	40.0	34.0	40.0
30	37.686281254517354	40.0	37.0	40.0	33.0	40.0
31	37.64539298853779	40.0	37.0	40.0	33.0	40.0
32	37.58744106724719	40.0	37.0	40.0	33.0	40.0
33	37.519772187500784	40.0	36.0	40.0	33.0	40.0
34	37.44717366497313	40.0	36.0	40.0	33.0	40.0
35	37.369607620042885	40.0	36.0	40.0	33.0	40.0
36	37.3126077027653	40.0	36.0	40.0	33.0	40.0
37	37.18582349600495	40.0	35.0	40.0	33.0	40.0
38	37.01258606124633	40.0	35.0	40.0	32.0	40.0
39	36.896130178889294	39.0	35.0	40.0	32.0	40.0
40	36.77682691881868	39.0	35.0	40.0	32.0	40.0
41	36.642487120232424	39.0	35.0	40.0	31.0	40.0
42	36.46173002358285	39.0	35.0	40.0	31.0	40.0
43	36.3172014424337	39.0	35.0	40.0	31.0	40.0
44	36.15887632331482	38.0	35.0	40.0	31.0	40.0
45	35.97614363729594	38.0	35.0	40.0	30.0	40.0
46	35.84102758499867	38.0	35.0	40.0	30.0	40.0
47	35.69311852617078	38.0	35.0	40.0	30.0	40.0
48	35.556063650019034	37.0	35.0	40.0	30.0	40.0
49	35.400598999365236	37.0	35.0	40.0	29.0	40.0
50	35.26350819327854	37.0	34.0	40.0	29.0	40.0
51	35.112351513973266	37.0	34.0	40.0	29.0	40.0
52	34.406461427822194	35.0	33.0	39.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	1.0
14	9.0
15	17.0
16	43.0
17	103.0
18	338.0
19	698.0
20	1348.0
21	2454.0
22	4115.0
23	6351.0
24	9523.0
25	13887.0
26	19602.0
27	26844.0
28	35653.0
29	46893.0
30	59735.0
31	75514.0
32	92723.0
33	117059.0
34	169353.0
35	316122.0
36	286996.0
37	550892.0
38	1092729.0
39	661317.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.000767062332308	20.340994579593303	35.879048140820835	17.779190217253554
2	23.846753535408116	20.53415279580851	37.16088338619304	18.458210282590333
3	24.03999530961159	20.910915764913497	36.28917859990792	18.759910325566988
4	26.439613616721182	21.45510108984684	38.018522577786214	14.08676271564576
5	0.0	0.0	0.0	100.0
6	0.0	100.0	0.0	0.0
7	0.0	0.0	100.0	0.0
8	100.0	0.0	0.0	0.0
9	0.0	100.0	0.0	0.0
10	0.0	0.0	100.0	0.0
11	99.5019108319284	0.3819714170404262	0.041862552122776765	0.07425519890839845
12	99.50029537748854	0.3213640228826336	0.14458317236815316	0.03375742726068226
13	97.85370166065931	1.140343718570011	0.37740358034838667	0.6285510404222909
14	57.96233818647414	29.478756913379055	4.771968857380719	7.786936042766092
15	34.71873406305453	23.885385178651156	20.48098206260666	20.914898695687654
16	29.59821698394099	17.878679928619196	27.826258432045492	24.696844655394322
17	24.27462614067099	17.26840023496506	29.63152876859757	28.82544485576638
18	25.325339990491102	18.458321693241356	30.322887563535406	25.89345075273214
19	25.600969941127826	18.772109791854266	27.904524414390803	27.72239585262711
20	29.39998958310413	18.821548268246765	24.382276682224237	27.396185466424868
21	31.282718174781586	20.542480741972653	21.004417153786527	27.17038392945923
22	30.704747569924805	20.725417030956287	22.1713880179516	26.398447381167312
23	29.36765264164402	20.78365694877979	21.55305890476088	28.295631504815304
24	29.486611364276342	21.605310500091772	21.982964754404968	26.925113381226918
25	31.25873703214838	20.90094451164673	23.144755023297357	24.69556343290753
26	31.377361522827623	20.47092725135162	21.63956927528207	26.512141950538687
27	30.12401955145515	21.55634551896613	21.97602944137864	26.34360548820008
28	30.36672765471388	21.739421071263546	20.75811605703223	27.135735216990348
29	29.356985071808346	21.24759875231212	20.859471896802543	28.535944279076997
30	29.57172910165971	21.22294914577276	20.82941887368845	28.375902878879078
31	29.210173686419683	20.72926069841666	21.44788725019295	28.612678364970705
32	29.28415035870052	22.742256193805513	19.6603312071539	28.31326224034007
33	30.26138888416941	22.56617165985994	20.860808824614846	26.311630631355804
34	29.28080803916976	21.660013129745224	21.422764148386733	27.63641468269829
35	30.99118435371099	22.601516688897732	20.097200222487068	26.3100987349042
36	32.044711322469496	22.758271474890407	20.25626677948852	24.940750423151577
37	32.09284072371245	22.541048558053724	20.80769379673851	24.558416921495322
38	29.70333293318341	24.330916372101548	22.74479078611634	23.2209599085987
39	29.512904277918327	25.20432017081481	22.41456961647719	22.868205934789675
40	29.78870134453159	24.9004197674804	20.011915369127163	25.29896351886085
41	30.324697986614567	24.83181865911154	19.06631746854947	25.777165885724422
42	31.445266314627577	24.75795339748173	19.545828910562594	24.2509513773281
43	31.487351688052406	24.29339883536876	19.42726012520886	24.791989351369974
44	29.636737216533003	24.628884158268857	20.658960577619663	25.075418047578474
45	28.10874013772028	24.33718322122172	20.674307394798404	26.879769246259595
46	27.637110999267193	22.64789137238704	22.328811267850423	27.38618636049534
47	28.199289144341133	21.94795395732025	22.891184381563654	26.96157251677496
48	28.205583846124068	21.42209568448058	23.074064965221773	27.29825550417358
49	28.802410703666887	21.010154802314336	22.472001807080762	27.71543268693802
50	28.315239779395768	22.42877447448292	22.01789199350142	27.23809375261989
51	28.3597483344804	22.011374470416435	23.054735217268874	26.57414197783429
52	28.896051355853697	22.055994436152087	22.791889638837308	26.256064569156905
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	4.0
8	8.0
9	64.5
10	121.0
11	286.5
12	452.0
13	990.5
14	1758.5
15	1988.0
16	2298.0
17	2608.0
18	2892.0
19	3176.0
20	4162.5
21	5149.0
22	6801.0
23	8453.0
24	10784.5
25	13116.0
26	16619.5
27	20123.0
28	22149.0
29	24175.0
30	27079.5
31	29984.0
32	31231.0
33	32478.0
34	34238.0
35	35998.0
36	42207.0
37	48416.0
38	49550.5
39	60013.5
40	69342.0
41	77834.5
42	86327.0
43	97615.5
44	108904.0
45	128149.0
46	147394.0
47	158107.5
48	168821.0
49	178567.0
50	188313.0
51	209471.5
52	230630.0
53	254184.5
54	277739.0
55	297509.0
56	317279.0
57	321985.5
58	326692.0
59	327772.0
60	328852.0
61	313487.5
62	298123.0
63	262870.5
64	198008.0
65	168398.0
66	147816.5
67	127235.0
68	111089.5
69	94944.0
70	79569.5
71	64195.0
72	54859.5
73	45524.0
74	33055.5
75	20587.0
76	14612.0
77	8637.0
78	6145.5
79	3654.0
80	2985.0
81	2316.0
82	1235.5
83	155.0
84	125.0
85	95.0
86	84.0
87	73.0
88	44.0
89	7.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	3590321.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.122702493021114
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.76882422239714	6.073249149115227
2	7.952175981260409	1.2918631933585312
3	3.7319942911998636	0.9094163799920909
4	2.332465217313431	0.7578368414222737
5	1.4689162851965207	0.5965784985902546
6	1.029942034688931	0.5019547639701012
7	0.6975878004401412	0.39664087160153755
8	0.4851473889948716	0.31525663248570623
9	0.36355440042026876	0.26577398111782624
>10	2.538043278568193	4.232531770095741
>50	0.9114192125857815	5.5544379849851
>100	3.442946898416975	63.31830877889991
>500	0.25315278127590457	13.34453045109028
>1k	0.02383020724152444	2.4416207032754396
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.004985626633384591	0.0	0.0	0.0	0.0
12	0.010333337882601584	0.0	0.0	0.0	0.0
13	0.01930189529014258	0.0	0.0	0.0	0.0
14	0.057794275219402384	0.0	0.0	0.0	0.0
15	0.0841428941868986	0.0	0.0	0.0	0.0
16	0.10065952320140734	0.0	0.0	0.0	0.0
17	0.11135494569984132	0.0	0.0	0.0	0.0
18	0.12837292264396416	0.0	0.0	0.0	0.0
19	0.14182575875527564	0.0	0.0	0.0	0.0
20	0.15859306173459142	0.0	0.0	0.0	0.0
21	0.17742146175787624	0.0	0.0	0.0	0.0
22	0.1956928085260343	0.0	0.0	0.0	0.0
23	0.2144933558865628	0.0	0.0	0.0	0.0
24	0.23499291567522793	0.0	0.0	0.0	0.0
25	0.2562723500210705	0.0	0.0	0.0	0.0
26	0.28036490330530334	0.0	0.0	0.0	0.0
27	0.3052094784839573	0.0	0.0	0.0	0.0
28	0.33044399094119997	0.0	0.0	0.0	0.0
29	0.36021848742772583	0.0	0.0	0.0	0.0
30	0.39946288925140677	0.0	0.0	0.0	0.0
31	0.43366595911619044	0.0	0.0	0.0	0.0
32	0.4661421638900811	0.0	0.0	0.0	0.0
33	0.4893712846288674	0.0	0.0	0.0	0.0
34	0.5123497314028467	0.0	0.0	0.0	0.0
35	0.5336291657486893	0.0	0.0	0.0	0.0
36	0.5539059042353037	0.0	0.0	0.0	0.0
37	0.574823253965314	0.0	0.0	0.0	0.0
38	0.5983030486689073	0.0	0.0	0.0	0.0
39	0.6226741285807035	0.0	0.0	0.0	0.0
40	0.6509445812783871	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACTA	30	1.862516E-6	46.0	30
CGTATAT	20	6.3132547E-4	46.0	25
TTATGCG	30	1.862516E-6	46.0	21
TAACGTA	30	1.862516E-6	46.0	43
CCGTATA	20	6.3132547E-4	46.0	24
CCGTAAA	30	1.862516E-6	46.0	46
CGTACAC	45	3.110472E-10	46.0	40
TCGTAAT	25	3.418616E-5	46.0	34
ACGTCGA	25	3.418616E-5	46.0	26
CATAACG	25	3.418616E-5	46.0	19
CGTAAAC	40	5.6170393E-9	46.0	38
ATACGTA	25	3.418616E-5	46.0	17
ATACGCT	55	1.8189894E-12	46.0	26
TTCGATA	25	3.418616E-5	46.0	30
TCGGTAC	25	3.418616E-5	46.0	36
TACGTTG	25	3.418616E-5	46.0	46
GTACGTT	30	1.862516E-6	46.0	20
CTAATAG	30	1.862516E-6	46.0	43
TACGTAC	20	6.3132547E-4	46.0	18
TAATCGC	45	3.110472E-10	46.0	18
>>END_MODULE