FastQCFastQC Report
Fri 17 Jun 2016
SRR1527908_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527908_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3566792
Sequences flagged as poor quality0
Sequence length52
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAA206.313248E-446.00000444
TAACGTA206.313248E-446.00000443
CCGTAAA206.313248E-446.00000446
TACGATA206.313248E-446.00000428
AATACGT206.313248E-446.00000416
AACGTTA501.6370905E-1146.033
TCGATAG301.8625124E-646.026
CTACGTT301.8625124E-646.045
TCGTAAT501.6370905E-1146.034
CGTAAAC301.8625124E-646.038
ACGTAAC253.4186123E-546.034
TACACGT301.8625124E-646.018
GCGTAAA301.8625124E-646.037
TACGTTG301.8625124E-646.046
GTACGTT453.110472E-1046.020
TAGTACG900.046.040
CGCGATA351.0203803E-745.99999640
CGTACCG351.0203803E-745.99999638
ATACGTA351.0203803E-745.99999617
CTAATCG351.0203803E-745.99999631
Produced by FastQC (version 0.11.5)