FastQCFastQC Report
Fri 17 Jun 2016
SRR1527907_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527907_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3619967
Sequences flagged as poor quality0
Sequence length59
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG203.1391467E-453.00000450
CCGTATA203.1391467E-453.00000424
CTTATCG401.6025297E-953.00000430
CATAACG203.1391467E-453.00000419
CGTAACG203.1391467E-453.00000418
ACCGTAC203.1391467E-453.00000436
CGGTATA203.1391467E-453.00000427
CGTATAA457.6397555E-1153.039
TCCGATA150.006697423353.047
TACGATA503.6379788E-1253.028
CGTAAAT600.053.051
ACGTAAC353.3447577E-853.034
TCATACG600.053.052
ATACGTA700.053.017
TTACCGC251.4793699E-553.019
GCGTATT353.3447577E-853.015
ATCGAAC457.6397555E-1153.028
ACGCGTA457.6397555E-1153.026
CGCATGA209200.051.163243
CACGCAT15950.050.8401261
Produced by FastQC (version 0.11.5)