##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527907_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3619967
Sequences flagged as poor quality	0
Sequence length	59
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.512528429126565	31.0	31.0	34.0	30.0	34.0
2	31.981730220192613	33.0	31.0	34.0	30.0	34.0
3	32.181870166219745	34.0	31.0	34.0	30.0	34.0
4	35.84938923476374	37.0	35.0	37.0	35.0	37.0
5	35.82191605614084	37.0	35.0	37.0	35.0	37.0
6	35.92120618779121	37.0	35.0	37.0	35.0	37.0
7	36.083977561121415	37.0	35.0	37.0	35.0	37.0
8	35.85548487044219	37.0	35.0	37.0	33.0	37.0
9	36.77398302249717	39.0	37.0	39.0	32.0	39.0
10	37.54719531973634	39.0	37.0	39.0	35.0	39.0
11	37.93401928802113	39.0	37.0	39.0	35.0	39.0
12	37.966093889806174	39.0	38.0	39.0	35.0	39.0
13	37.941207475095766	39.0	38.0	39.0	35.0	39.0
14	38.34829875520965	40.0	38.0	40.0	35.0	40.0
15	37.73585753682285	40.0	38.0	40.0	33.0	40.0
16	37.18597904345537	39.0	36.0	40.0	32.0	40.0
17	37.07894408982181	39.0	36.0	40.0	32.0	40.0
18	37.06299173445504	39.0	36.0	40.0	32.0	40.0
19	37.18394394203041	40.0	36.0	40.0	32.0	40.0
20	37.317663116818466	40.0	36.0	40.0	32.0	40.0
21	37.26532589937975	40.0	36.0	40.0	32.0	40.0
22	37.25884351984424	40.0	36.0	40.0	32.0	40.0
23	37.20383086365152	40.0	36.0	40.0	32.0	40.0
24	37.15784425659129	39.0	36.0	40.0	32.0	40.0
25	37.10633494725228	39.0	36.0	40.0	32.0	40.0
26	36.968897782769844	39.0	36.0	40.0	31.0	40.0
27	36.86093077644078	39.0	36.0	40.0	31.0	40.0
28	36.87018583318577	39.0	35.0	40.0	31.0	40.0
29	36.83981870552964	39.0	35.0	40.0	31.0	40.0
30	36.793953094047545	39.0	35.0	40.0	31.0	40.0
31	36.780645790417424	39.0	35.0	40.0	31.0	40.0
32	36.66304748081958	39.0	35.0	40.0	31.0	40.0
33	36.53245071018603	39.0	35.0	40.0	30.0	40.0
34	36.47468333274861	39.0	35.0	40.0	30.0	40.0
35	36.33806219780457	39.0	35.0	40.0	30.0	40.0
36	36.17796432950908	39.0	35.0	40.0	30.0	40.0
37	36.04655567302133	38.0	35.0	40.0	30.0	40.0
38	35.850843115420666	38.0	35.0	40.0	29.0	40.0
39	35.706712519755015	38.0	35.0	40.0	29.0	40.0
40	35.5514547508306	38.0	34.0	40.0	28.0	40.0
41	35.44607174595791	38.0	34.0	40.0	28.0	40.0
42	35.289825017741876	38.0	34.0	40.0	28.0	40.0
43	35.11000956638555	38.0	34.0	40.0	27.0	40.0
44	34.93746351831384	37.0	34.0	40.0	26.0	40.0
45	34.703209725392526	37.0	33.0	40.0	26.0	40.0
46	34.526910051942465	37.0	33.0	40.0	26.0	40.0
47	34.403970809678654	36.0	33.0	40.0	25.0	40.0
48	34.222559763666354	36.0	33.0	40.0	24.0	40.0
49	34.096613864159536	36.0	33.0	39.0	24.0	40.0
50	33.90938563804587	36.0	33.0	39.0	24.0	40.0
51	33.75372178807155	35.0	33.0	39.0	24.0	40.0
52	33.56240043072216	35.0	33.0	39.0	23.0	40.0
53	33.317170570891946	35.0	32.0	39.0	23.0	40.0
54	33.12123287311735	35.0	32.0	38.0	23.0	40.0
55	32.95979162240982	35.0	32.0	38.0	22.0	40.0
56	32.74896677234903	35.0	32.0	38.0	22.0	40.0
57	32.58120225957861	35.0	31.0	38.0	22.0	40.0
58	32.391054393589776	35.0	31.0	37.0	21.0	40.0
59	31.78943261084977	34.0	30.0	37.0	20.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	4.0
13	2.0
14	20.0
15	62.0
16	247.0
17	702.0
18	1547.0
19	2990.0
20	5363.0
21	8545.0
22	12946.0
23	18360.0
24	25453.0
25	32904.0
26	42440.0
27	53671.0
28	65117.0
29	77649.0
30	92309.0
31	111913.0
32	137322.0
33	173801.0
34	243548.0
35	347148.0
36	397334.0
37	613316.0
38	900975.0
39	254278.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.04233685003206	20.60582320225571	35.41062667145861	17.941213276253624
2	23.556402585990423	21.077263964008512	36.8682366441462	18.49809680585486
3	23.893670853905576	21.1005514691156	36.11455574042526	18.891221936553563
4	26.520462755599706	21.69787735634054	37.610315232155436	14.171344655904322
5	0.0	0.0	0.0	100.0
6	0.0	100.0	0.0	0.0
7	0.0	0.0	100.0	0.0
8	100.0	0.0	0.0	0.0
9	0.0	100.0	0.0	0.0
10	0.0	0.0	100.0	0.0
11	99.48505055432825	0.3934290008721074	0.047127501438549026	0.07439294336108589
12	99.47629356842204	0.33246159426315214	0.15339918844564052	0.037845648869174776
13	97.81470383569794	1.1628282799263086	0.39019692720955745	0.6322709571661841
14	57.65732118552462	29.719221197320305	4.799629388886694	7.82382822826838
15	34.7317254549558	23.956931099095655	20.47010373298983	20.84123971295871
16	29.66864062572946	18.001683440760647	27.70514206344975	24.62453387006014
17	24.300967384509306	17.28612443152106	29.53040732139271	28.882500862576926
18	25.170395199735246	18.563926135238248	30.338591484397508	25.927087180628995
19	25.651062564934985	18.84008887373835	27.923320847952482	27.58552771337418
20	29.36849976809181	18.939040052022573	24.41723916267745	27.275221017208167
21	30.986995185315223	20.67927691053537	21.10267856032942	27.231049343819986
22	30.664837552386526	20.85513486725155	22.193903977577698	26.286123602784222
23	29.338803364782056	20.956903750780047	21.56265512917659	28.141637755261307
24	29.358030059390046	21.76287794888738	22.039013062826264	26.84007892889631
25	31.165090731490093	21.097208897208176	23.160321627241352	24.577378744060375
26	31.30478813757142	20.615519423243363	21.714617840438876	26.36507459874634
27	29.99433420249411	21.704092882614674	22.090339497570007	26.211233417321207
28	30.317016702085958	21.813016527498732	20.843974544519327	27.025992225895983
29	29.345295136668376	21.353564825314706	20.855770232159575	28.445369805857347
30	29.489992588330228	21.355443295477556	20.886074375816133	28.268489740376086
31	29.121784811850493	20.896709831885207	21.417239438923062	28.564265917341235
32	29.113248822434016	22.99656322833882	19.710290176678406	28.179897772548756
33	30.113561808712618	22.783909356079764	20.935052722856312	26.167476112351302
34	29.321455140336916	21.83395594490226	21.427405277451424	27.4171836373094
35	30.948569420660466	22.768964468460624	20.129271896677512	26.153194214201402
36	31.914876572079248	22.957861218071876	20.314908948064996	24.81235326178388
37	32.10043074978308	22.668853058605233	20.826653944635407	24.404062246976284
38	29.651209527600663	24.502543807719796	22.78349498766149	23.06275167701805
39	29.345764754209085	25.391004945625195	22.468050123108856	22.795180177056864
40	29.59513166832736	25.22017465905076	19.973248374916125	25.211445297705755
41	30.261960951577738	25.1092896703202	19.032079574206062	25.596669803896006
42	31.33694312682961	25.038681291846032	19.46056414326429	24.16381143806007
43	31.323213719904075	24.512737270809374	19.441420322339955	24.722628686946592
44	29.597894124449205	24.760391462132112	20.617839886385706	25.02387452703298
45	28.006581275464665	24.47422863247096	20.638392559932175	26.8807975321322
46	27.481106871968723	22.850208303003868	22.31205422590869	27.35663059911872
47	28.07641616622472	22.109731939545306	22.82415834177494	26.989693552455034
48	28.175118723457977	21.617407009511414	22.946369400605032	27.26110486642558
49	28.706366660248563	21.23497258400422	22.45680692669298	27.60185382905424
50	28.20467700396164	22.683659823418278	22.003349754293342	27.10831341832674
51	28.24522985983021	22.179843075917542	23.051094112183897	26.52383295206835
52	28.693272618231052	22.229484412426963	22.876506885283764	26.200736084058224
53	28.729074049570062	22.227412570335588	23.064657771742116	25.97885560835223
54	26.49278294525889	22.68479243042823	22.883081530853737	27.93934309345914
55	26.578446709597074	22.862832727480665	23.227615058369317	27.331105504552944
56	27.799037947031007	22.7036876303016	22.31440231361225	27.18287210905514
57	27.31127106959815	21.301326780050758	23.879665201367857	27.507736948983236
58	28.042465580487335	21.945421049418407	22.70236165136312	27.30975171873114
59	27.405857567209868	21.698623219493438	22.204318437157024	28.69120077613967
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	0.5
5	0.0
6	4.5
7	9.0
8	61.0
9	227.5
10	342.0
11	730.5
12	1119.0
13	1193.0
14	1459.0
15	1651.0
16	1635.5
17	1620.0
18	1826.5
19	2362.5
20	2692.0
21	3787.0
22	4882.0
23	6283.0
24	7684.0
25	9713.5
26	14543.5
27	17344.0
28	19376.5
29	21409.0
30	25604.5
31	30010.0
32	30220.0
33	32048.5
34	33877.0
35	37900.5
36	46722.0
37	51520.0
38	52287.0
39	53054.0
40	56766.0
41	60478.0
42	72781.0
43	94246.5
44	103409.0
45	114850.0
46	126291.0
47	129919.0
48	140791.0
49	148035.0
50	161554.0
51	175073.0
52	198607.5
53	232966.0
54	243790.0
55	268289.5
56	292789.0
57	297528.5
58	304212.5
59	306157.0
60	280379.5
61	254602.0
62	232970.5
63	211339.0
64	192167.0
65	155439.0
66	137883.0
67	126902.0
68	115921.0
69	95540.5
70	67748.5
71	60337.0
72	49117.0
73	37897.0
74	28944.0
75	15033.5
76	10076.0
77	7597.0
78	5118.0
79	4049.5
80	2981.0
81	2201.5
82	948.5
83	475.0
84	373.0
85	271.0
86	189.0
87	64.0
88	21.0
89	12.5
90	4.0
91	2.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	3619967.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.274174473114412
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.91841989583327	9.932068038901901
2	7.020540543350156	1.7234267904930656
3	2.9787754595138862	1.0968602911891498
4	1.6879836454819739	0.828744230896378
5	1.059895219713152	0.650466942498958
6	0.6934219385310317	0.510670911420906
7	0.4673016317265733	0.40150192315681127
8	0.3424310915968292	0.3362447170622803
9	0.2384504758683505	0.2634104469604763
>10	1.5811523715257347	4.073670195556424
>50	0.630856173938434	5.855317503176608
>100	2.2152159422625153	60.231133635678006
>500	0.151683505918897	11.960841185030603
>1k	0.013872104739209471	2.1356431879784097
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.004944796458089259	0.0	0.0	0.0	0.0
12	0.010773578875166542	0.0	0.0	0.0	0.0
13	0.01922669460798952	0.0	0.0	0.0	0.0
14	0.053895518937051086	0.0	0.0	0.0	0.0
15	0.0772382731665786	0.0	0.0	0.0	0.0
16	0.09386825902004078	0.0	0.0	0.0	0.0
17	0.10464183789520733	0.0	0.0	0.0	0.0
18	0.11895136060632597	0.0	0.0	0.0	0.0
19	0.13127191490972157	0.0	0.0	0.0	0.0
20	0.1466035463859201	0.0	0.0	0.0	0.0
21	0.1633440304842558	0.0	0.0	0.0	0.0
22	0.18033313563355688	0.0	0.0	0.0	0.0
23	0.19784710744600711	0.0	0.0	0.0	0.0
24	0.2160516932889167	0.0	0.0	0.0	0.0
25	0.23657674227416992	0.0	0.0	0.0	0.0
26	0.2602786157995363	0.0	0.0	0.0	0.0
27	0.28273738407007576	0.0	0.0	0.0	0.0
28	0.3058315172486379	0.0	0.0	0.0	0.0
29	0.33550029599717346	0.0	0.0	0.0	0.0
30	0.3711083554076598	0.0	0.0	0.0	0.0
31	0.4030147236148838	0.0	0.0	0.0	0.0
32	0.4326282532409826	0.0	0.0	0.0	0.0
33	0.4542306601137524	0.0	0.0	0.0	0.0
34	0.4771314213637859	0.0	0.0	0.0	0.0
35	0.49771171947147586	0.0	0.0	0.0	0.0
36	0.5189826316096252	0.0	0.0	0.0	0.0
37	0.5372148420137531	0.0	0.0	0.0	0.0
38	0.5579608874887534	0.0	0.0	0.0	0.0
39	0.5807511504939133	0.0	0.0	0.0	0.0
40	0.6086795818856913	0.0	0.0	0.0	0.0
41	0.6377958694098592	0.0	0.0	0.0	0.0
42	0.6620778587208116	0.0	0.0	0.0	0.0
43	0.6847852480423164	0.0	0.0	0.0	0.0
44	0.7076031356086948	0.0	0.0	0.0	0.0
45	0.7296475354609586	0.0	0.0	0.0	0.0
46	0.7545372651187152	0.0	0.0	0.0	0.0
47	0.7767474123382893	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAG	20	3.1391467E-4	53.000004	50
CCGTATA	20	3.1391467E-4	53.000004	24
CTTATCG	40	1.6025297E-9	53.000004	30
CATAACG	20	3.1391467E-4	53.000004	19
CGTAACG	20	3.1391467E-4	53.000004	18
ACCGTAC	20	3.1391467E-4	53.000004	36
CGGTATA	20	3.1391467E-4	53.000004	27
CGTATAA	45	7.6397555E-11	53.0	39
TCCGATA	15	0.0066974233	53.0	47
TACGATA	50	3.6379788E-12	53.0	28
CGTAAAT	60	0.0	53.0	51
ACGTAAC	35	3.3447577E-8	53.0	34
TCATACG	60	0.0	53.0	52
ATACGTA	70	0.0	53.0	17
TTACCGC	25	1.4793699E-5	53.0	19
GCGTATT	35	3.3447577E-8	53.0	15
ATCGAAC	45	7.6397555E-11	53.0	28
ACGCGTA	45	7.6397555E-11	53.0	26
CGCATGA	20920	0.0	51.16324	3
CACGCAT	1595	0.0	50.840126	1
>>END_MODULE