FastQCFastQC Report
Fri 17 Jun 2016
SRR1527906_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527906_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5719
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC3976.941773037244274No Hit
CCTTTCCTTTATCAGCGGCAGACTTGCCACCAAGTCCAACCAAATCAAGC2123.7069417730372445No Hit
CCTTTCTTTTTGGGGTAATTATACTCATCGCGAATATCCTTAAGAGGGCG1863.2523168386081482No Hit
CCTTTCGGGTATACGAACCGAATGCTTCTATTATGAAAGCTGGCTGCTTT1723.007518796992481No Hit
CCTTTCGGGTCAAATAACACTTATAAGTCTTGACTGTTCAAAGGAATAAT1362.3780381185521944No Hit
CCTTTCGCCATCAACTAACGATTCTGTCAAAAACTGACGCGTTGGATGAG1312.290610246546599No Hit
CCTTTCCCATCTTGGCTTCCTTGCTGGTCAGATTGGTCGTCTTATTACCA1272.2206679489421224No Hit
CCTTTCGGGTAGAGGTTCCCAAGGTCCAAACTATTTTCACAATAATACTA1172.045812204930932No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCTTTCGGGA1131.9758699073264556No Hit
CCTTTCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAAC961.6786151425074316No Hit
CCTTTCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA951.6611295681063125No Hit
CCTTTCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGC791.381360377688407No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTT510.8917642944570728No Hit
CCTTTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCATTGTAGCGCC490.8567931456548347No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCAGCATTT490.8567931456548347No Hit
CCTTTCGGGGAAAAATAACTAATGGGTACTAGGCTTAATACCTGGGTGAT450.7868508480503584No Hit
CCTTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA420.7343941248470013No Hit
CCTTTCGGTGCACGTCGAAACTTGACGCGCTCGGATCATGTGCCGCTCGC420.7343941248470013No Hit
CCTTTCGGGGGGGAATTCCTTCGACTCTGTTTTGCTACCCGTTGTAGCGC410.7169085504458822No Hit
CCTTTCGGGGGATCTCAGTGGTGGTGGTGGTGGTGCTCGAGTGCGGCCGC400.699422976044763No Hit
CCTTTCGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCAGCATTTT340.5945095296380486No Hit
CCTTTCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGTGC330.5770239552369295No Hit
CCTTTCGGGGGGTCGAGGGTACCCCGGGAGGTGGCACGCTTGACGCTAAC280.4895960832313341No Hit
CCTTTCGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTT280.4895960832313341No Hit
CCTTTCGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA230.40216821122573876No Hit
CCTTTCGGGCAGATCCAGGGCCAGGCGTGATGGCTCATGCCGTAATCCCA220.3846826368246197No Hit
CCTTTCGGGGTGTAATATTAAAAATCCTACGATGTCGGCAAAATGCCTCA220.3846826368246197No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCG200.3497114880223815Illumina Single End Adapter 2 (100% over 23bp)
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCTTTCGGGG180.3147403392201434No Hit
CCTTTCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA180.3147403392201434Illumina Single End Adapter 2 (97% over 34bp)
CCTTTCGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.2972547648190243No Hit
CCTTTCGGGAAGCAGTGGTATCACGCAGTTCGGGAGAAAAAAAAAAAAAA170.2972547648190243No Hit
CCTTTCGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA160.2797691904179052Illumina Single End Adapter 2 (100% over 34bp)
CCTTTCGGGTGCTCCTTTCGGGGGGCGCGGAAGCTGTCTGGGGCGCCAAC160.2797691904179052No Hit
CCTTTCGGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA150.2622836160167862No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCTTTCGGGC140.24479804161566704No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCTTTCGGGT140.24479804161566704No Hit
CCTTTCAAATAAATGTTAATGTTGTCACCAAAAAAAAAAAAAAAAAAAAA130.227312467214548No Hit
CCTTTCGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA130.227312467214548Illumina Single End Adapter 2 (97% over 34bp)
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGATCGGAAGAGCTCGTATGC130.227312467214548Illumina Single End Adapter 2 (100% over 21bp)
CCTTTCGGGCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.20982689281342895No Hit
CCTTTCGGGGCAGTGCTCCTTTCGGGACTTTTTTTTTTTTTTTTTTTTTT110.19234131841230984No Hit
CCTTTCGGGAAGGCGTGAAAAAACCAAAGCGAAAAAAAGGACAACTCAAA100.17485574401119075No Hit
CCTTTCGGGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAAAA100.17485574401119075No Hit
CCTTTCGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.17485574401119075No Hit
CCTTTCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAG100.17485574401119075No Hit
CCTTTCGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA100.17485574401119075Illumina Single End Adapter 2 (97% over 34bp)
CCTTTCGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA90.1573701696100717Illumina Single End Adapter 2 (97% over 34bp)
CCTTTCTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC90.1573701696100717No Hit
CCTTTCGGGAAGCAGTGATACAACCCAGAGTACAGTGCTCCTTTCGGAAA90.1573701696100717No Hit
CCTTTCGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCCGCATTTT90.1573701696100717No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCCGCATTT80.1398845952089526No Hit
CCTTTCGGGAAGCAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAA80.1398845952089526Illumina Single End Adapter 2 (97% over 34bp)
CCTTTCGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.1398845952089526No Hit
CCTTTCGGGGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTC70.12239902080783352Illumina Single End Adapter 2 (100% over 28bp)
CCTTTCGGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCCGCAT70.12239902080783352No Hit
CCTTTCGGGTATACGAACCGAATGCTTCTATTATGAAAGCTGGCCGCTTT70.12239902080783352No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCAGCCTTT70.12239902080783352No Hit
CCTTTCGGGCAAAACCAATCAAAAAGGAGAAAACAAGATCACCCACGCCG70.12239902080783352No Hit
CCTTTCGGGAAACAGTGGTATCAAGAGTGCAGTGCTCTTTCGGGGAAAAA70.12239902080783352No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGGATCGGAAGAGCTCGTATG70.12239902080783352No Hit
CCTTTCGGGGTATCAACGCAGAGTACAGTACTCCTTTCGGGGAGGTTCCG70.12239902080783352No Hit
CCTTTCGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCCGCATTTT60.10491344640671448No Hit
CCTTTCGGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCAGCAT60.10491344640671448No Hit
CCTTTCGGGGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCT60.10491344640671448Illumina Single End Adapter 2 (96% over 29bp)
CCTTTCGGGCAGAGTGCAGTGCTCCTTTCGGGAAGCAGTGGTATCAACGC60.10491344640671448No Hit
CCTTTCGGGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCT60.10491344640671448Illumina Single End Adapter 2 (100% over 29bp)
CCTTTCGGGGGGAATTCGTGGAGAAAGAGATGGCTCGTCTGGCAGCATTT60.10491344640671448No Hit
CCTTTCGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG60.10491344640671448No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGTGTAGTGCTCCTTTCGAGC60.10491344640671448No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAT207.2149263E-444.00000429
TTATGAA302.1119959E-644.00000431
TTTTGGG207.2149263E-444.0000048
TCCTTAA207.2149263E-444.00000437
TGGCAGC207.2149263E-444.00000440
AACCGAA302.1119959E-644.00000416
TATACTC207.2149263E-444.00000420
TATACGA302.1119959E-644.00000410
TTTCTTT207.2149263E-444.0000043
GGGTATA302.1119959E-644.0000047
GGGTAAT207.2149263E-444.00000413
AGAGGGC207.2149263E-444.00000443
GCTGGCT302.1119959E-644.00000439
GCGAATA207.2149263E-444.00000430
AATTATA207.2149263E-444.00000417
TCGCGAA207.2149263E-444.00000428
TTTTTGG207.2149263E-444.0000047
ATGAAAG302.1119959E-644.00000433
TAAGAGG207.2149263E-444.00000441
GGCTGCT302.1119959E-644.00000442
Produced by FastQC (version 0.11.5)