FastQCFastQC Report
Fri 17 Jun 2016
SRR1527905_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527905_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7795
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG5597.171263630532393No Hit
CCTTTCGGGTATACGAACCGAATGCTTCTATTATGAAAGCTGGCTGCTTTGC2773.5535599743425275No Hit
CCTTTCGGGTCAAATAACACTTATAAGTCTTGACTGTTCAAAGGAATAATCT2032.6042334830019245No Hit
CCTTTCGGGTAGAGGTTCCCAAGGTCCAAACTATTTTCACAATAATACTAAG1742.2322001282873636No Hit
CCTTTCCTTTATCAGCGGCAGACTTGCCACCAAGTCCAACCAAATCAAGCAA1531.962796664528544No Hit
CCTTTCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACAT1481.8986529826812057No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCTTTCGGGAAG1381.7703656189865298No Hit
CCTTTCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCG1331.7062219371391918No Hit
CCTTTCGCCATCAACTAACGATTCTGTCAAAAACTGACGCGTTGGATGAGGA871.1161000641436818No Hit
CCTTTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCATTGTAGCGCCGG841.077613855035279No Hit
CCTTTCTTTTTGGGGTAATTATACTCATCGCGAATATCCTTAAGAGGGCGTT841.077613855035279No Hit
CCTTTCGGGGAAAAATAACTAATGGGTACTAGGCTTAATACCTGGGTGATGA831.0647851186658115No Hit
CCTTTCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA801.0262989095574084No Hit
CCTTTCCCATCTTGGCTTCCTTGCTGGTCAGATTGGTCGTCTTATTACCATT660.846696600384862No Hit
CCTTTCGGTGCACGTCGAAACTTGACGCGCTCGGATCATGTGCCGCTCGCTC650.8338678640153944No Hit
CCTTTCGGGGGGGAATTCCTTCGACTCTGTTTTGCTACCCGTTGTAGCGCCG610.7825529185375241No Hit
CCTTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA510.654265554842848No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTTA470.6029506093649776No Hit
CCTTTCAAATAAATGTTAATGTTGTCACCAAAAAAAAAAAAAAAAAAAAAAA450.5772931366260423No Hit
CCTTTCGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTTAT440.5644644002565747No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTGA440.5644644002565747No Hit
CCTTTCGGGGGATCTCAGTGGTGGTGGTGGTGGTGCTCGAGTGCGGCCGCTT430.5516356638871072No Hit
CCTTTCGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTGAT420.5388069275176395No Hit
CCTTTCGGGGGGTCGAGGGTACCCCGGGAGGTGGCACGCTTGACGCTAACGT410.5259781911481719No Hit
CCTTTCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGTGCCG390.5003207184092368No Hit
CCTTTCGGGCAGATCCAGGGCCAGGCGTGATGGCTCATGCCGTAATCCCAGC310.3976908274534958No Hit
CCTTTCGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.3720333547145606No Hit
CCTTTCGGGGTGTAATATTAAAAATCCTACGATGTCGGCAAAATGCCTCAAA290.3720333547145606No Hit
CCTTTCGGTTATTTGGCTTGTCTTTTCCTGCACCCTCAACTGAGTGGCTAAT270.3463758819756254No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGA260.3335471456061578No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC220.28223220012828737Illumina Single End Adapter 2 (100% over 25bp)
CCTTTCGGGAAGCAGTGGTATCACGCAGTTCGGGAGAAAAAAAAAAAAAAAA220.28223220012828737No Hit
CCTTTCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA210.26940346375881974Illumina Single End Adapter 2 (97% over 34bp)
CCTTTCGGGGCAGTGCTCCTTTCGGGACTTTTTTTTTTTTTTTTTTTTTTTT210.26940346375881974No Hit
CCTTTCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG210.26940346375881974No Hit
CCTTTCGGGAAGGCGTGAAAAAACCAAAGCGAAAAAAAGGACAACTCAAAAA210.26940346375881974No Hit
CCTTTCGGGGTATCAACGCAGAGTACAGTACTCCTTTCGGGGAGGTTCCGCG190.24374599101988453No Hit
CCTTTCGGGTGCTCCTTTCGGGGGGCGCGGAAGCTGTCTGGGGCGCCAACCA190.24374599101988453No Hit
CCTTTCGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCCGCATTTTAT170.21808851828094933No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCTTTCGGGCAG160.20525978191148175No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCTTTCGGGGAG150.19243104554201412No Hit
CCTTTCGGGAAGCAGTGGTATCAACAGATCGGAAGAGCTCGTATGCCGTCTT150.19243104554201412Illumina Single End Adapter 2 (100% over 27bp)
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTA150.19243104554201412No Hit
CCTTTCGGGTATACGAACCGAATGCTTCTATTATGAAAGCTGGCCGCTTTGC150.19243104554201412No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGGATCGGAAGAGCTCGT140.1796023091725465No Hit
CCTTTCGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.1796023091725465No Hit
CCTTTCGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGAT140.1796023091725465No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCAGCATTTTA140.1796023091725465No Hit
CCTTTCGGGGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAAAAA130.1667735728030789No Hit
CCTTTCGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.1667735728030789No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCTTTTGGGAAG130.1667735728030789No Hit
CCTTTCGGGCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.1539448364336113No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGT120.1539448364336113Illumina Single End Adapter 2 (95% over 24bp)
CCTTTCGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.1539448364336113No Hit
CCTTTCGGGGAAGCAGTGATATCAACGCAGATTCGGGTAAAAAAAAAAAAAA120.1539448364336113No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTTA110.14111610006414368No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCT110.14111610006414368Illumina Single End Adapter 2 (96% over 26bp)
CCTTTCGGGCAAAACCAATCAAAAAGGAGAAAACAAGATCACCCACGCCGCC110.14111610006414368No Hit
CCTTTCGGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTT100.12828736369467605No Hit
CCTTTCGGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.12828736369467605No Hit
CCTTTCGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA100.12828736369467605Illumina Single End Adapter 2 (97% over 34bp)
CCTTTCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGA100.12828736369467605No Hit
CCTTTCGGGAAGTCAACGCAGAGTACAGTGCTCCTTTCGGGAAAAAAAAAAA90.11545862732520845No Hit
CCTTTCGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTTAT90.11545862732520845No Hit
CCTTTCGGGGGGTCGAGGGTACCCCGGGAGGGGGCACGCTTGACGCTAACGT90.11545862732520845No Hit
CCTTTCGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCAGCATTTTAT90.11545862732520845No Hit
CCTTTCGGGGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.11545862732520845No Hit
CCTTTCGGGAAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAA90.11545862732520845Illumina Single End Adapter 2 (100% over 34bp)
CCTTTCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG90.11545862732520845No Hit
CCTTTCGGGAAACAGTGGTATCAAGAGTGCAGTGCTCTTTCGGGGAAAAAAA90.11545862732520845No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCCGCATTTTA80.10262989095574088No Hit
CCTTTCGGGCAGAGTGCAGTGCTCCTTTCGGGAAGCAGTGGTATCAACGCAG80.10262989095574088No Hit
CCTTTCGGGAAGCAGTCATATCAACGCAGAGTGCATTACTCTTTCGGGAAAA80.10262989095574088No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGATCGGAAGAGCTCGTATGCCG80.10262989095574088Illumina Single End Adapter 2 (100% over 23bp)
CCTTTCGGGGAGAGTGCAGTGCTCCTTTCGGCGACTTTTTTTTTTTTTTTTT80.10262989095574088No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCTTTCGGGTAG80.10262989095574088No Hit
CCTTTCGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGGAGCATTTTAT80.10262989095574088No Hit
CCTTTCGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA80.10262989095574088Illumina Single End Adapter 2 (100% over 34bp)
CCTTTCGGGTAGTGCAGTGCTCCTTTCGGGTGGTATCAACGCAAAAAAAAAA80.10262989095574088No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTCCT205.929571E-446.0000042
ACTGTTC205.929571E-446.00000433
GGATGAG205.929571E-446.00000444
AAACTGA205.929571E-446.00000431
ACTAACG205.929571E-446.00000414
GGGTCAA205.929571E-446.0000047
ATAAGTC205.929571E-446.00000423
AACGATT205.929571E-446.00000417
TTCGCCA205.929571E-446.0000044
TGACTGT205.929571E-446.00000431
CGGGGTG404.476533E-946.0000046
ACTGACG205.929571E-446.00000433
CGGGGGT205.929571E-446.0000046
TTCAAAG205.929571E-446.00000437
AACTGAC205.929571E-446.00000432
CGGGGAG205.929571E-446.0000046
TAAGTCT205.929571E-446.00000424
CAACTAA205.929571E-446.00000412
GTCAAAT205.929571E-446.0000049
GTCAAAA205.929571E-446.00000426
Produced by FastQC (version 0.11.5)