FastQCFastQC Report
Fri 17 Jun 2016
SRR1527904_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527904_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7894
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG5026.359260197618443No Hit
CCTTTCGGGTATACGAACCGAATGCTTCTATTATGAAAGCTGGCTGCTTTGC3053.8636939447681784No Hit
CCTTTCGGGTCAAATAACACTTATAAGTCTTGACTGTTCAAAGGAATAATCT1702.153534329870788No Hit
CCTTTCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACAT1622.0521915378768685No Hit
CCTTTCCTTTATCAGCGGCAGACTTGCCACCAAGTCCAACCAAATCAAGCAA1451.8368381048897895No Hit
CCTTTCGGGTAGAGGTTCCCAAGGTCCAAACTATTTTCACAATAATACTAAG1411.78616670889283No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCTTTCGGGAAG1321.6721560678996705No Hit
CCTTTCTTTTTGGGGTAATTATACTCATCGCGAATATCCTTAAGAGGGCGTT1161.4694704839118318No Hit
CCTTTCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCG991.254117050924753No Hit
CCTTTCGCCATCAACTAACGATTCTGTCAAAAACTGACGCGTTGGATGAGGA911.1527742589308336No Hit
CCTTTCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA841.064099315936154No Hit
CCTTTCCCATCTTGGCTTCCTTGCTGGTCAGATTGGTCGTCTTATTACCATT811.0260957689384342No Hit
CCTTTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCATTGTAGCGCCGG801.0134279199391942No Hit
CCTTTCGGGGAAAAATAACTAATGGGTACTAGGCTTAATACCTGGGTGATGA760.9627565239422345No Hit
CCTTTCGGGGGGGAATTCCTTCGACTCTGTTTTGCTACCCGTTGTAGCGCCG610.7727387889536357No Hit
CCTTTCGGTGCACGTCGAAACTTGACGCGCTCGGATCATGTGCCGCTCGCTC580.7347352419559159No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTTA560.709399543957436No Hit
CCTTTCGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTTAT550.6967316949581961No Hit
CCTTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA540.6840638459589561No Hit
CCTTTCGGGGGGTCGAGGGTACCCCGGGAGGTGGCACGCTTGACGCTAACGT460.5827210539650367No Hit
CCTTTCAAATAAATGTTAATGTTGTCACCAAAAAAAAAAAAAAAAAAAAAAA450.5700532049657968No Hit
CCTTTCGGGGGATCTCAGTGGTGGTGGTGGTGGTGCTCGAGTGCGGCCGCTT450.5700532049657968No Hit
CCTTTCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGTGCCG430.5447175069673169No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTGA350.44337471497339753No Hit
CCTTTCGGGCAGATCCAGGGCCAGGCGTGATGGCTCATGCCGTAATCCCAGC290.36736762097795794No Hit
CCTTTCGGGTGCTCCTTTCGGGGGGCGCGGAAGCTGTCTGGGGCGCCAACCA290.36736762097795794No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCTTTCGGGGAG240.3040283759817583No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC220.2786926779832784Illumina Single End Adapter 2 (100% over 25bp)
CCTTTCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA220.2786926779832784Illumina Single End Adapter 2 (97% over 34bp)
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGA210.2660248289840385No Hit
CCTTTCGGGGTGTAATATTAAAAATCCTACGATGTCGGCAAAATGCCTCAAA200.25335697998479856No Hit
CCTTTCGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.2280212819863187No Hit
CCTTTCGGTTATTTGGCTTGTCTTTTCCTGCACCCTCAACTGAGTGGCTAAT180.2280212819863187No Hit
CCTTTCGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTGAT170.21535343298707882No Hit
CCTTTCGGGGCAGTGCTCCTTTCGGGACTTTTTTTTTTTTTTTTTTTTTTTT170.21535343298707882No Hit
CCTTTCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG160.20268558398783887No Hit
CCTTTCGGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTT150.19001773498859895No Hit
CCTTTCGGGAAGCAGTGGTATCACGCAGTTCGGGAGAAAAAAAAAAAAAAAA150.19001773498859895No Hit
CCTTTCGGGGAGAGTGCAGTGCTCCTTTCGGCGACTTTTTTTTTTTTTTTTT150.19001773498859895No Hit
CCTTTCGGGAAGCAGTGGTATCAACAGATCGGAAGAGCTCGTATGCCGTCTT150.19001773498859895Illumina Single End Adapter 2 (100% over 27bp)
CCTTTCGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.19001773498859895No Hit
CCTTTCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGA150.19001773498859895No Hit
CCTTTCGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCCGCATTTTAT140.177349885989359No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGT130.16468203699011907Illumina Single End Adapter 2 (95% over 24bp)
CCTTTCGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTTAT130.16468203699011907No Hit
CCTTTCGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCCTTTTAT130.16468203699011907No Hit
CCTTTCGGGAAGCAGTGATACAACCCAGAGTACAGTGCTCCTTTCGGAAAAA130.16468203699011907No Hit
CCTTTCGGGGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTG120.15201418799087915Illumina Single End Adapter 2 (100% over 30bp)
CCTTTCGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.15201418799087915No Hit
CCTTTCGGGGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAAAAA110.1393463389916392No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCTTTCGGGCAG110.1393463389916392No Hit
CCTTTCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG110.1393463389916392No Hit
CCTTTCGGGAAACAGTGGTATCAAGAGTGCAGTGCTCTTTCGGGGAAAAAAA110.1393463389916392No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTA110.1393463389916392No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCCTTTTA100.12667848999239928No Hit
CCTTTCGGGAAGGCGTGAAAAAACCAAAGCGAAAAAAAGGACAACTCAAAAA100.12667848999239928No Hit
CCTTTCGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAG90.11401064099315936No Hit
CCTTTCGGGAAGCAGTCATATCAACGCAGAGTGCATTACTCTTTCGGGAAAA90.11401064099315936No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTTA90.11401064099315936No Hit
CCTTTCGGAAGGTGGTATCAATGCAGAGTGTGCTCCTTTCGGGAAAAAAAAA90.11401064099315936No Hit
CCTTTCGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA90.11401064099315936Illumina Single End Adapter 2 (100% over 34bp)
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGGAGCATTTTA90.11401064099315936No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCAGCATTTGA90.11401064099315936No Hit
CCTTTCGGGTATACGAACCGAATGCTTCTATTATGAAAGCTGGCCGCTTTGC90.11401064099315936No Hit
CCTTTCGGGCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.10134279199391943No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGGATCGGAAGAGCTCGT80.10134279199391943No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCCGCATTTTA80.10134279199391943No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGATCGGAAGAGCTCGTATGCCG80.10134279199391943Illumina Single End Adapter 2 (100% over 23bp)
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGAGATCGGAAGAGCTCGTATGC80.10134279199391943Illumina Single End Adapter 2 (100% over 21bp)
CCTTTCGGGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAAAAAA80.10134279199391943No Hit
CCTTTCGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCAGCATTTTAT80.10134279199391943No Hit
CCTTTCGGGTAGCGGTTCCCAAGGTCCAAACTATTTTCACAATAATACTAAG80.10134279199391943No Hit
CCTTTCGGGGTATCAACGCAGAGTACAGTACTCCTTTCGGGGAGGTTCCGCG80.10134279199391943No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGGGCAGTGCTCCTTTCCGGCAG80.10134279199391943No Hit
CCTTTCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAGA80.10134279199391943No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCTTTCGGGTAG80.10134279199391943No Hit
CCTTTCAACCAATACTCTGTCAAAGAGCGGAAGAAACTTCCTAAAAGCTTGT80.10134279199391943No Hit
CCTTTCGGGCAAAACCAATCAAAAAGGAGAAAACAAGATCACCCACGCCGCC80.10134279199391943No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGGAGCATTTGA80.10134279199391943No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTCCT205.9343665E-446.0000042
TCCTTTA205.9343665E-446.0000045
CTTTATC205.9343665E-446.0000047
TCAAGCA205.9343665E-446.00000445
TTTCCTT205.9343665E-446.0000043
AAATCAA205.9343665E-446.00000442
CCAAATC205.9343665E-446.00000440
ACTTGCC205.9343665E-446.00000422
CGGGGGA800.046.0000046
GTCGTCT205.9343665E-446.00000436
CGGGGAA205.9343665E-446.0000046
AATCAAG205.9343665E-446.00000443
TTATCAG205.9343665E-446.0000049
GCGGCAG205.9343665E-446.00000415
TGCTGAA205.9343665E-446.00000440
ACCAAGT205.9343665E-446.00000429
ACCAAAT205.9343665E-446.00000439
TTCCTTT205.9343665E-446.0000044
ATCAGCG205.9343665E-446.00000411
TCAGCGG205.9343665E-446.00000412
Produced by FastQC (version 0.11.5)