FastQCFastQC Report
Fri 17 Jun 2016
SRR1527903_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527903_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences373883
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT37270.9968359085596297No Hit
CAACAAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC29380.785807324751326No Hit
CAACAAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA17450.46672354720594406No Hit
CAACAAGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC10700.2861857853927566No Hit
CAACAAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC10450.2794992016218978No Hit
CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10430.27896427492022907No Hit
CAACAAGGGGAACCTGGCGCTAAACCATTCGTAGACGACCTGCTTCTGGG10380.2776269581660573No Hit
CAACAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC9340.24981076967928467No Hit
CAACAAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC8460.22627399480586174No Hit
CAACAAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA8180.21878502098249988No Hit
CAACAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7840.20969126705413188No Hit
CAACAAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA7420.19845780631908913No Hit
CAACAAGGGATGTGTGTATGTATGTGTGTCTGTGTGTGTCTGTGTGTCCA7300.1952482461090769No Hit
CAACAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA7210.19284107595156774No Hit
CAACAAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG7170.1917712225482303No Hit
CAACAAGGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCG7000.18722434558404635No Hit
CAACAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6540.17492103144566618No Hit
CAACAAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCG6320.1690368377273104No Hit
CAACAAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC6260.1674320576223043No Hit
CAACAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC6100.16315264400895468No Hit
CAACAAGGGAGATTTCTACCACTCCAAAAAAAAAAAAAAAAAAAAAAAAA6080.16261771730728597No Hit
CAACAAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC6000.16047801050061117No Hit
CAACAAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGA5850.15646606023809587No Hit
CAACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG5760.1540588900805867No Hit
CAACAAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG5710.15272157332641495No Hit
CAACAAGGGGGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC5130.13720869897802254No Hit
CAACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGATTG5050.1350689921713477No Hit
CAACAAGGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGT5010.1339991387680103No Hit
CAACAAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG5000.13373167541717595No Hit
CAACAAGGGGACGGGGGTAGCGTGGCCGAGCGGTCTAAGGCGCTGGATTA4990.13346421206634163No Hit
CAACAAGGGGAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT4900.13105704190883244No Hit
CAACAAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAG4850.12971972515466068No Hit
CAACAAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT4810.12864987175132328No Hit
CAACAAGGGGAGCATCAGTTCCTGGGTCCTGTCCGAAGGCACGCTCAGGA4800.12838240840048892No Hit
CAACAAGGGGAGCACAGCTGGCCATCAGAGTCACCAATCCCAACGCCAGG4780.12784748169882024No Hit
CAACAAGGGGGAGCTGGTGAGATGGCTCCGCAGTTAAGAGCACTGACTAC4730.12651016494464848No Hit
CAACAAGGGAGTGAGCACACTGCTTCAAATAAGGTCATGTGTGAAAGAAC4710.12597523824297976No Hit
CAACAAGGGTAGCAAATCTACTCAAGAATGACCCCATGGTCTGTGGGGTG4630.12383553143630495No Hit
CAACAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG4520.12089343457712706No Hit
CAACAAGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAAC4510.12062597122629272No Hit
CAACAAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC4430.1184862644196179No Hit
CAACAAGGGGAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAG4340.11607909426210875No Hit
CAACAAGGGGGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTG4270.11420685080626826No Hit
CAACAAGGGGTCTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTA4210.11260207070126216No Hit
CAACAAGGGAAGGAAAAGGCAGAGAAGCTGAAGCAGTCCCTACCCCCGGG4180.11179968064875911No Hit
CAACAAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG4170.11153221729792474No Hit
CAACAAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT4140.1107298272454217No Hit
CAACAAGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCT4010.10725280368457511No Hit
CAACAAGGGGGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATC3910.1045781701762316No Hit
CAACAAGGGGACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGGA3890.1040432434745629No Hit
CAACAAGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCTGACTGC3880.10377578012372855No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGACTA650.044.00000443
TAGCACG650.044.00000422
CTAGCAC650.044.00000421
TAATCAC207.849659E-444.023
GCGAGAA207.849659E-444.020
TCGCCCT254.4370605E-544.042
TATTTCG207.849659E-444.037
AACGTTT207.849659E-444.021
ACTATTC551.8189894E-1244.042
AACCGTA207.849659E-444.013
CGGAACT408.287316E-944.033
GGTACTC254.4370605E-544.09
TCGTTGA207.849659E-444.014
TACCCGT351.4425859E-744.035
ACACGCA207.849659E-444.020
CATATTC254.4370605E-544.041
GATAATG207.849659E-444.010
CATATGA207.849659E-444.025
GTCGATT254.4370605E-544.044
GTCGATG254.4370605E-544.042
Produced by FastQC (version 0.11.5)